2005 Fiscal Year Final Research Report Summary
The systematic approach for the understanding of dynamic aspects of chromosome VI of S.cerevisiae
| Project/Area Number |
15310136
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
基礎ゲノム科学
|
|---|
| Research Institution | Tokyo Institute of Technology (2004-2005)
The Institute of Physical and Chemical Research (2003) |
|---|
Principal Investigator |
SHIRAHIGE Katsuhiko Tokyo Institute of Technology, Division of Gene Research Center for Biological, Assistant Professor, バイオ研究基盤支援総合センター, 助教授 (90273854)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Keywords | ChIP-chip / DNA array / cohesin / Smc5 / 6 / cell cycle / chromosome / DNA chip / chromatin immuno-precipitation |
|---|
| Research Abstract |
The goal of this proposal is to build up the system that enables us to study the dynamic aspects of chromosome behavior through the analyses of protein binding profile data (ChIP-chip data) of various DNA binding proteins on S.cerevisiae chromosome VI (280kb).
We have developed the software for calculation and visual presentation of the protein binding profile on chromosome VI. All of these software are available through our web site (http://shirahigelab.bio.titech.ac.jp). Now we are trying to expand the system so that analyses at whole genome level is possible for S.cerevisiae, S.pombe, and H.sapiens. Our analytical method is 100% compatible with MIAME checklist and other research groups utilized our system for the publication of their own data. All these suggest that the system we have built is now going to be a standard of ChIP-chip technique world wide (Methods in Enzymology, 2006). Proto-type system for the comparison and classification of protein binding profile data is also launched, and we have made the new achievements as follows using this new system. 1) Discovery of regulator of cohesin complex (nature, 2006), 2) Identification of Scc2, a cohesin loader, as a repair protein, 3) Dynamics of cohesin during S-phase (coupling of cohesin binding to chromosome with chromosomal DNA replication), 4) Identification of specific binding profile of SMC5/6 (Molecular Cell, 2006), and so on. All of these new findings identified cohesin as a key molecule that links various chromosome functions.
|
