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2005 Fiscal Year Final Research Report Summary

The systematic approach for the understanding of dynamic aspects of chromosome VI of S.cerevisiae

Research Project

Project/Area Number 15310136
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 基礎ゲノム科学
Research InstitutionTokyo Institute of Technology (2004-2005)
The Institute of Physical and Chemical Research (2003)

Principal Investigator

SHIRAHIGE Katsuhiko  Tokyo Institute of Technology, Division of Gene Research Center for Biological, Assistant Professor, バイオ研究基盤支援総合センター, 助教授 (90273854)

Project Period (FY) 2003 – 2005
KeywordsChIP-chip / DNA array / cohesin / Smc5 / 6 / cell cycle / chromosome / DNA chip / chromatin immuno-precipitation
Research Abstract

The goal of this proposal is to build up the system that enables us to study the dynamic aspects of chromosome behavior through the analyses of protein binding profile data (ChIP-chip data) of various DNA binding proteins on S.cerevisiae chromosome VI (280kb).
We have developed the software for calculation and visual presentation of the protein binding profile on chromosome VI. All of these software are available through our web site (http://shirahigelab.bio.titech.ac.jp). Now we are trying to expand the system so that analyses at whole genome level is possible for S.cerevisiae, S.pombe, and H.sapiens. Our analytical method is 100% compatible with MIAME checklist and other research groups utilized our system for the publication of their own data. All these suggest that the system we have built is now going to be a standard of ChIP-chip technique world wide (Methods in Enzymology, 2006). Proto-type system for the comparison and classification of protein binding profile data is also launched, and we have made the new achievements as follows using this new system. 1) Discovery of regulator of cohesin complex (nature, 2006), 2) Identification of Scc2, a cohesin loader, as a repair protein, 3) Dynamics of cohesin during S-phase (coupling of cohesin binding to chromosome with chromosomal DNA replication), 4) Identification of specific binding profile of SMC5/6 (Molecular Cell, 2006), and so on. All of these new findings identified cohesin as a key molecule that links various chromosome functions.

  • Research Products

    (4 results)

All 2005

All Journal Article (4 results)

  • [Journal Article] Identification and characterization of rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae : Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles.2005

    • Author(s)
      K.Unno, P.R.Juvvadi, H.Nakajima, K.Shirahige, K.Kitamoto
    • Journal Title

      FEMS Yeast Res 5

      Pages: 801-812

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae2005

    • Author(s)
      H.Tamaki, C.W.Yun, T.Mizutani, T.Tsuzuki, Y.Takagi, M.Shinozaki, Y.Kodama, K.Shirahige, H.Kumagai.
    • Journal Title

      Genes Cells. 10

      Pages: 193-206

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification and characterization of rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae : Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles.2005

    • Author(s)
      K.Unno, P.R.Juvvadi, H.Nakajima, K.Shirahige, K.Kitamoto.
    • Journal Title

      FEMS Yeast Res 5

      Pages: 801-812

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae2005

    • Author(s)
      H.Tamaki, C.W.Yun, T.Mizutani, T.Tsuzuki, Y.Takagi, M.Shinozaki, Y.Kodama, K.Shirahige, H.Kumagai
    • Journal Title

      Genes Cells. 10

      Pages: 193-206

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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