2004 Fiscal Year Final Research Report Summary
DEVELOPMENT OF CAPILLARY AND MICROCHIP SEPARATION-DETECTION SYSTEMS TOWARD PROTEOMICS
| Project/Area Number |
15350041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
OTSUKA Koji KYOTO UNIVERSITY, GRADUATE SCHOOL OF ENGINEERING, PROFESSOR, 工学研究科, 教授 (70183762)
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| Co-Investigator(Kenkyū-buntansha) |
HAGINAKA Jun MUKOGAWA WOMEN'S UNIVERSITY, SCHOOL OF PHARMACEUTICAL SCIENCES, PROFESSOR, 薬学部, 教授 (20164759)
KITAGAWA Fumihiko KYOTO UNIVERSITY, GRADUATE SCHOOL OF ENGINEERING, ASSISTANT PROFESSOR, 工学研究科, 助手 (20362452)
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| Project Period (FY) |
2003 – 2004
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| Keywords | protein / capillary electrophoresis(CE) / microchip electrophoresis / mass spectrometry(MS) / isoelectric focusing / chiral separation / CE-MS / thermal lens microscopy |
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| Research Abstract |
Toward the high performance separation and detection of proteins by capillary electrophoresis(CE) and microchip electrophoresis(MCE), following five subjects were mainly investigated.
1)Development of a high performance analysis method by microchip isoelectric focusing(MCIEF). Four model proteins were successfully separated within 210 s by MCIEF, where good linear relationship between a pI value of the protein and its focused position.
2)Development of a highly efficient chiral separation technique by capillary electrochromatography and microchip electrochromatography using immobilized proteins. Avidin was used as a chiral selector and four pairs of enantiomers were successfully resolved.
3)Improvement of detection sensitivity in MCE using on-line sample preconcentration. Under a micellar electrokinetic chromatography (MEKC) mode in MCE, successful on-line sample preconcentration by SRW was achieved by using a newly designed and fabricated T -cross type channel quartz chip.
4)Improvement of detectability in CE and MCE by using thermal lens microscopy (TLM). In a microchip MEKC-TLM system with sweeping as an on-line sample preconcentration technique, the limit of detection of 4 nM or 8 amol in absolute amount injected for Sunset Yellow was obtained.
5)Development of a high performance MCE system with mass spectrometric (MS) detection. An MCE-MS system with an electrospray ionization interface was developed, where some peptides and proteins were successfully separated and detected.
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[Journal Article] Application of thermal lens microscopy and on-line sample preconcentration for high sensitive detection in microchip electrophoresis2004
Author(s)
F.Kitagawa, K.Sueyoshi, J.Mizuno, Y.Wada, S.Shoji, K.Otsuka
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Journal Title
Micro Total Analysis Systems 2004(Vol.1.1)(Laurell, T. ; Nilsson, J. ; Jensen, K. ; Harrison, D.J. ; Kutter, J.P.,Eds.)(Royal Society of Chemistry (Cambridge, UK)) Vol.1
Pages: 67-69
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Designing multifunctional microchips for electrophoretic analysis2004
Author(s)
K.Sueyoshi, H.Nagai, S.Wakida, J.Nishii, F.Kitagawa, K.Otsuka
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Journal Title
Micro Total Systems 2004(Vol.1.1)(Laurell.T. ; Nilsson, J. ; Jensen, K. ; Harrison, D.J. ; Kutter, J.P., Eds.)(Royal Society of Chemistry (Cambridge, UK)) Vol.1
Pages: 86-88
Description
「研究成果報告書概要(欧文)」より
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[Book] Micro Total Analysis Systems 2004 (Vol.1 ; pp.67-69)(Laurell, T.; Nilsson, J.; Jensen, K.; Harrison, D.J.; Kutter, J.P., Eds.)2004
Author(s)
F.Kitagawa, K.Sueyoshi, J.Mizuno, Y.Wada, S.Shoji, K.Otsuka
Total Pages
684
Publisher
Royal Society of Chemistry (Cambridge, UK)
Description
「研究成果報告書概要(和文)」より
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[Book] Micro Total Analysis Systems 2004 (Vol.1 ; pp.86-88)(Laurell, T.; Nilsson, J.; Jensen, K.; Harrison, D.J.; Kutter, J.P., Eds.)2004
Author(s)
K.Sueyoshi, H.Nagai, S.Wakida, J.Nishii, F.Kitagawa, K.Otsuka
Total Pages
684
Publisher
Royal Society of Chemistry (Cambridge, UK)
Description
「研究成果報告書概要(和文)」より
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