2004 Fiscal Year Final Research Report Summary
DEVELOPMENT OF HIGHLY ACCURATE SENSORS USING MOLECULAR IMPRINTING TECHNIQUE AND THEIR APPLICATIONS TO BIO-RELATED COMPOUNDS
| Project/Area Number |
15350044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | OSAKA PREFECTURE UNIVERSITY |
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Principal Investigator |
NAGAOKA Tsutomu OSAKA PREFECTURE UNIVERSITY, RESEARCH INSTITUTE OF ADVANCED SCIENCE AND TECHNOLOGY, PROFESSOR, 先端科学研究所, 教授 (00172510)
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| Co-Investigator(Kenkyū-buntansha) |
HORII Toyokazu OSAKA PREFECTURE UNIVERSITY, RESEARCH INSTITUTE OF ADVANCED SCIENCE AND TECHNOLOGY, LECTURER, 先端科学研究所, 講師 (00100214)
MORI Nobuko OSAKA PREFECTURE UNIVERSITY, RESEARCH INSTITUTE OF ADVANCED SCIENCE AND TECHNOLOGY, LECTURER, 先端科学研究所, 講師 (90100221)
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| Project Period (FY) |
2003 – 2004
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| Keywords | SAM / MOLECULAR IMPRINT / SENSOR / OVEROXIDIZED POLYPYRROLE / CHOLESTEROL / BILE ACIDS / SKIN / EXTRACTION |
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| Research Abstract |
Molecular imprinting techniques, which have developed during the last decade, are becoming a powerful tool for the preparation of artificial polymers that recognize target molecules. However, this approach has still important problems in selectivity, which has been discouraging its use in sensor technology. We have been applying the overoxidation technique for the preparation of polypyrrole (PPy) films in order to solve such a problem. In this project, we have developed highly selective sensors using the above and self-assembly techniques for applications to bio-related compounds.
Alkanethiol adsorbed on a Au micro-electrode was used as the base element for this type of sensors. In this case, cholesterol was imprinted in the alkanethiol monolayer, and this electrode was applied to measure the skin cholesterol level of human. An overoxidized polypyrrole film imprinted with bile acid was prepared on a 9-MHz Au-EQCM electrode. The sensor could pick up signals arising from bile acid as low as 25 nM levels.
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