2004 Fiscal Year Final Research Report Summary
Molecular mechanism for circadian clock molecular machinery at the stomic level
| Project/Area Number |
15370004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Nagoya University |
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Principal Investigator |
ISHIURA Masahiro Nagoya University, Center for Gene research, Professor, 遺伝子実験施設, 教授 (20132730)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Yasuo Nagoya University, Center for Gene research, Associate Professor, 遺伝子実験施設, 助教授 (70154507)
IHARA Kunio Nagoya University, Center for Gene research, Research Associate, 遺伝子実験施設, 助手 (90223297)
九町 健一 名古屋大学, 大学院・生命農学研究科, 学術振興会研究員
NAMBA Keiichi Osaka. University, Graduate School of Frontier Bioscience, Professor, 大学院・生命機能研究科, 教授 (30346142)
KATO Hiroaki Kyoto University, Graduate School of Pharmacology, Professor, 大学院・薬学研究科, 教授 (90204487)
KUCHO Kenich Nagoya University, Graduate School of Agricultural Science, Postdoctoral fellow
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| Project Period (FY) |
2003 – 2004
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| Keywords | Biological clock / Molecular machinery / Cyanobacteria / Clock proteins / Clock genes / Arabidopsis / Bioluminescence / Real-time monitoring |
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| Research Abstract |
The cyanobacteria clock machinery is composed of three Kai proteins, KaiA, KaiC, and KaiC. We found that KaiC forms hexmers in the presence of ATP and clarified the three-dimensional structure of KaiC hexamer by electron microscopic observation followed by single particle analysis. We determined the stoichiomertic interactions between KaiA and KaiC. We determined the crystal structure of the C-terminal clock oscillator domain of KaiC and identified His270 as a functional residue. Based on the structure, we demonstrated the structure-function relationships of KaiA. We determined the different roles of the two domains, N-terminal and C-terminal domains, of KaiC.
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[Journal Article] Global analysis of circadian expression in the cyanobacterium Synechocystis sp.strain PCC 6803.2005
Author(s)
Kucho, K., Okamoto, K., Tsuchiya, Y., Nomura, S., Nango, M., Kanehisa, M., Ishiura, M.
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Journal Title
J.Bacteriol. 187
Pages: 2190-2199
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Construction of unmodified oligonucleotide-based microarray in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1.2004
Author(s)
Kucho, K., Tsuchiya, Y., Okumoto, Y., Harada, M., Yamada, M., Ishiura.M.
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Journal Title
Genes Genet.Syst. 79
Pages: 319-329
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Roles of two ATPase-motif-containing domains in cyanobacterial circadian clock protein KaiC.2004
Author(s)
Hayashi, F., Ito, N., Uzumaki, T., Iwase, R., Tsuchiya, Y., Yamakawa, H., Morishita, M., Onai, K., Itoh, S., Ishiura, M.
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Journal Title
J.Biol.Chem. 279
Pages: 52331-52337
Description
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[Journal Article] Large-scale screening of Arabidopsis circadian clock mutants by a high-throughput real-time bioluminescence monitoring system.2004
Author(s)
Onai, K., Okamoto, K., Nishimoto, H., Morioka, C., Hirano, M., Kami-ike, N., Ishiura, M.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein.2004
Author(s)
Uzumaki, T., Fujita, M., Nakatsu, T., Hayashi, F., Shibata, H., Itoh, N., Kato, H., Ishiura, M.
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Journal Title
Nature Struct.Mol.Biol. 11
Pages: 623-631
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1.2004
Author(s)
Iwase, R., Imada, K., Hayashi, F., Uzumaki, T., Namba, K., Ishiura.M.
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Journal Title
Acta Cryst.D 60
Pages: 727-729
Description
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[Journal Article] Stoichiometric interactions between cyanobacterial clock proteins KaiA and KaiC.2004
Author(s)
Hayashi, F., Ito, H., Fujita, M., Iwase, R., Uzumaki, T., Ishiura.M.
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Journal Title
Biochem.Biophys.Res.Commun. 316
Pages: 195-202
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] ATP-induced hexameric ring structure of the cyanobacterial circadian clock protein KaiC.2003
Author(s)
Hayashi, F., Suzuki, H., Iwase, R., Uzumaki, T., Miyake, A., Shen, J.-R., Imada, K., Furukawa, Y., Yonekura, K., Namba, K., Ishiura, M.
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Journal Title
Genes Cells 8
Pages: 287-296
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of a new cryptochrome class : structure, function, and evolution.2003
Author(s)
Brudler, R., Hitomi, K., Daiyasu, H., Toh, H., Kucho, K., Ishiura, M., Kanehisa, M., Roberts, V.A., Todo, T., Tainer, J.A., Getzoff, E.D.
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Description
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