2004 Fiscal Year Final Research Report Summary
Importance of copper chaperone Cox17p in early embryogenesis.
| Project/Area Number |
15370050
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KAKO Koichiro University of Tsukuba, Graduate School of Life and Environmental Sciences, Assistant Professor (60311594)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Hiroko Teikyo University of School of Medicine, Department of Pediatrics, Associate Professor (00093386)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Cox17p / mitochondria / cytochrome c oxidase / Sco1 / 2p / central nervous system / in situ hybridization |
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| Research Abstract |
It is well known that the deficiencies in cytochrome c oxidase (CCO) are relatively rare but most often lethal. CCO, one of the mitochodrial respiratory enzymes, requires Cu (I) to express its enzymatic activity. Cox17p, Scolp, and its homologue Sco2p are thought to be involved in mitochondrial transport of copper to CCO. By in vitro GST-pull down assay, both Sco1p and Sco2p interact with Cox17p. Furthermore, Cox17p, Scolp and Sco2p also interact with Cox2p, a subunit of the binuclear-copper centre Cu_A of CCO. Northern blot analysis demonstrated that Cox17p, Sco1p and Sco2p are widely expressed at varying levels in a variety of mouse tissues including brain. Using in situ hybridization histochemistry, we characterized the expression profiles for these molecules in different regions of the normal adult mouse brain. The highest levels of these messages were associated with distinct neuronal subtypes, including pyramidal cell layers of hippocampus and ventral cochlear nucleus of pons, that were also contained high levels of CCO activity. Although CCO activities and expression levels of Cox17p and Scolp were relatively high, the expression of Sco2p was quite low in some subsets of neurons such as cerebellum Purkinje cells. It was thought that the difference of expression profile between Sco1p and Sco2p is originated in that of genomic structures, especially the promoter regions.
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Research Products
(14 results)
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[Journal Article] Transcriptional down-regulation through nuclear exclusion of EWS methylated by PRMT12005
Author(s)
Araya, N., Hiraga, H., Kako, K., Arao, Y., Kato, S., Fukamizu, A.
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Journal Title
Biochem.Biophys.Res.Commun. 329
Pages: 653-660
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A selective requirement for copper-dependent activation of cytochrome coxidase by Cox17p2004
Author(s)
Kako, K., Takehara, A., Arai, H., Onodera, T., Takahashi, Y., Hanagata, H., Ogra, Y., Takagi, H., Kodama, H., Suzuki, K.T., Munekata, E., Fukamizu, A.
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Journal Title
Biochem.Biophys.Res.Commun. 324
Pages: 1379-1385
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cox17p plays a key role for maintenance of cellular copper homeostasis2003
Author(s)
Kako, K., Onodera, T., Arai, H., Takehara, A., Hanagata, H., Takahashi, Y., Takagi, H., Kodama, H., Munekata, E.
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Journal Title
Peptide science 2002(Yamada, T., Ed.)(Protein Research Foundation, Osaka)
Pages: 119-122
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Highly conserved Drosophila ananassae timeless gene functions as a clock component in Drosophila melanogaster2003
Author(s)
Nishinokubi, I., Shimoda, M., Kako, K., Sakai, T., Fukamizu, A., Ishida, N.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Histochemical analysis of copper chaperone proteins-Cox17p, Sco1p and Sco2p-in different regions of the mouse brain2003
Author(s)
Kako, K., Takahashi, Y., Inada, Y., Hisano, S., Munekata, E., Fukamizu, A.
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Journal Title
Biomed.Res.Trace Elements 14
Pages: 284-286
Description
「研究成果報告書概要(欧文)」より
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