Principle design of reaction complex based on functions and structure of newly descovered photosynthesis.

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 15370067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: ITOH Shigeru Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (40108634)
• Co-Investigator(Kenkyū-buntansha): MINO Hiroyuki Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (70300902) ; SHIBATA Yutaka Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (20300832) ; NAKAMURA Yoko Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (20217912)
• Project Period (FY): 2003 – 2004
• Keywords: Photosynthesis / cyanobacteria / silica mesoscopic compounds / World Exposition '06 / arctic plants / photosynthetic bacteria / Zn-chlorophyll / artificial photosynthesis

Research Abstract

Structure and function of a wide variety of the photosynthetic reaction center pigment protein complexes, including those of newly discovered ones, are studied by ultra last laser spectroscopy up to 0.1 ps, fluorescence life time measurements at cryogenic temperature, electron spin resonance and by the single molecular confocal microscope spectroscopy. The biomaterials used were (1)Acaryochloris marina that can evolve oxygen even with near far-red light, (2)Zn-bacteriochlorophyll containing Acidiphillium rubrum, (3)Zooxanthellae that lives in corals, (4)photosynthetic green sulfur bacterium Chlorobium tepidum ant its site-directed mutants, (5)Chloroflexus aurantiacus, (6)cyanobacteria that are modified to be depleted of a phospholipid phosphatydil glycerol. Acaryochloris marina is shown to have a special photosystems I and II reaction centers in which chlorophyll d but not chlorophyll a functions as the special pair primary electron donor chlorophylls. This organism, thus, is an only one exceptional case in the oxygen evolving photosynthesis that can use 700-750 nm near far-red light. This makes a clear contrast to all the other oxygenic organisms that absorb only 650-690 nm red ight with chlorophyll a. A bacterium Acidiphilium rubrum uses Zn-bacteriochlorophyll a and was shown to undergo highly efficient photo-energy conversion even in the pico-to femto-second time ranges. These new photosynthesis indicated that pigment can be replaced rather easily in photosynthesis although now it is rather rare. On the other hand, the modifications of reaction center proteins yielded drastic decrease of efficiency indicating that the structure of the reaction center is highly optimized for the reaction. Reacion centers are shown to be functional even inside silica mesoscopic nanopores. A part of research to hunt for new organisms was exhibited with arctic plants from Alaska in Aichi World Exposition '06.

Research Products

• [Journal Article] Primary intermediate in the photocycle of a blue-light sensory BLUF FAD-protein, Tll0078, of Th (2005)
  • Author(s): Fukushima, Y., Okajima, K., Shibata, Y., IK
  • Journal Title: Biochemistr 44 Pages: 5149-5158
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of (2005)
  • Author(s): Okajima, K., Yoshihara, S., Fukushima, Y., Geng, X., Katayama, M., Higashi, S., Watanabe, M., Sato, S.
  • Journal Title: J.Biochem 137(in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Primary intermediate in the photocycle of a blue-light sensory BLUF FAD-protein, T110078,of Thermosynechococcus elongatus BP-1. (2005)
  • Author(s): Fukushima, Y., Okajima, K., Shibata, Y., Ikeuchi, M., Itoh, S.
  • Journal Title: Biochemistry 44 Pages: 5149-5158
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria. (2005)
  • Author(s): Okajima, K., Yoshihara, S., Fukushima, Y., Geng, X., Katayama, M., Higashi, S., Watanabe, M., Sato, S., Tabata, S., Shibata, Y., Itoh, S., Ikeuchi, M.
  • Journal Title: J.Biochem. 137(in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nanoscale Organization of Chlorophyll a in Mesoporous Silica : Efficient Energy Transfer and Stabilized Charge Separation as in Natural Photosynthesis (2004)
  • Author(s): Itoh, T., Yano, K., Kajino, T., Itoh.S., Shibata, Y., Mino, H., Inada, Y.
  • Journal Title: J.Phys.Chem.B 108 Pages: 13683-13687
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation : Regulation of energy transfer (2004)
  • Author(s): Tsukatani, Y., Miyamoto, R., Itoh.S., Oh-oka, H.
  • Journal Title: J.Biol.Chem 279 Pages: 51122-511300
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phosphatidylglycerol is essential for oligomerization of photosystem I reaction center. (2004)
  • Author(s): Domonkos, I., Malec, P., Sallai, A., Kovacs, L., I
  • Journal Title: Plant Physiol. 134 Pages: 1471-1478
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Roles of two ATPase-motif-containing domains in cyanobacerial circadian clock protein KaiC. ansfer and ferredoxin-mediated NADP+reduction on the cytoplasmic side. (2004)
  • Author(s): Hayashi, F., Itoh, N., Uzumaki, T., Iwase, R., Tsuchiya, Y., Yamakawa, H., Morishita, M., Onai
  • Journal Title: J.Biol.Chem 279 Pages: 52331-52337
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Nanoscale Organization of Chlorophyll a in Mesoporous Silica : Efficient Energy Transfer and Stabilized Charge Separation as in Natural Photosynthesis. (2004)
  • Author(s): Itoh, T., Yano, K., Kajino, T., Itoh, S., Shibata, Y., Mino, H., Inada, Y, Iwai, S., Fukushima, Y.
  • Journal Title: J.Phys.Chem.B 108 Pages: 13683-13687
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation ; Regulation of energy transfer and ferredoxin-mediated NADP+ reduction on the cytoplasmic side. (2004)
  • Author(s): Tsukatani, Y., Miyamoto, R., Itoh, S., Oh-oka, H.
  • Journal Title: J.Biol.Chem. 279 Pages: 51122-51130
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phosphatidylglycerol is essential for oligomerization of photosystem I reaction center. (2004)
  • Author(s): Domonkos, I., Malec, P., Sallai, A., Kovacs, L., Itoh, K., Shen, G., Ughy, B., Bogos, B., Sakurai, I., Kis, M., Strzalka, K., Wada, H., Itoh, S., Farkas, T., Gombos, Z.
  • Journal Title: Plant Physiol. 134 Pages: 1471-1478
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Roles of two ATPase-motif-containing domains in cyanobacerial circadian clock protein KaiC. (2004)
  • Author(s): Hayashi, F., Itoh, N., Uzumaki, T., Iwase, R., Tsuchiya, Y, Yamakawa, H., Morishita, M., Onai, K., Itoh, S., Ishiura, M.
  • Journal Title: J.Biol.Chem. 279 Pages: 52331-52337
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fluorescence of photosysntem I (2004)
  • Author(s): Itoh, S., Sugiura, K.
  • Journal Title: Chlorophyll fluorescence(ed.by C.Papapgeorgeiou and Govindjee)(Kluwer Academic Publishers, The Netherland) 750 Pages: 231-250
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Chlorophyll fluorescence : A signature of photosynthesis (2004) (ed. by C.Papapgeorgeiou and Govindiee) (2004)
  • Author(s): Itoh, S., Sugiura.K (共著)
  • Total Pages: 750
  • Publisher: Kluwer Academic Publishers, The Netherland
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 膜タンパク質複合材料及びその製造方法 (2004)
  • Inventor(s): 梶野 勉他7名
  • Industrial Property Rights Holder: 名大, トヨタ自, 豊田中研
  • Industrial Property Number: 特願2004-358900
  • Filing Date: 2004-12-10
  • Description: 「研究成果報告書概要(和文)」より