• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2004 Fiscal Year Final Research Report Summary

Principle design of reaction complex based on functions and structure of newly descovered photosynthesis.

Research Project

Project/Area Number 15370067
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionNagoya University

Principal Investigator

ITOH Shigeru  Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (40108634)

Co-Investigator(Kenkyū-buntansha) MINO Hiroyuki  Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (70300902)
SHIBATA Yutaka  Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (20300832)
NAKAMURA Yoko  Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (20217912)
Project Period (FY) 2003 – 2004
KeywordsPhotosynthesis / cyanobacteria / silica mesoscopic compounds / World Exposition '06 / arctic plants / photosynthetic bacteria / Zn-chlorophyll / artificial photosynthesis
Research Abstract

Structure and function of a wide variety of the photosynthetic reaction center pigment protein complexes, including those of newly discovered ones, are studied by ultra last laser spectroscopy up to 0.1 ps, fluorescence life time measurements at cryogenic temperature, electron spin resonance and by the single molecular confocal microscope spectroscopy. The biomaterials used were (1)Acaryochloris marina that can evolve oxygen even with near far-red light, (2)Zn-bacteriochlorophyll containing Acidiphillium rubrum, (3)Zooxanthellae that lives in corals, (4)photosynthetic green sulfur bacterium Chlorobium tepidum ant its site-directed mutants, (5)Chloroflexus aurantiacus, (6)cyanobacteria that are modified to be depleted of a phospholipid phosphatydil glycerol. Acaryochloris marina is shown to have a special photosystems I and II reaction centers in which chlorophyll d but not chlorophyll a functions as the special pair primary electron donor chlorophylls. This organism, thus, is an only one exceptional case in the oxygen evolving photosynthesis that can use 700-750 nm near far-red light. This makes a clear contrast to all the other oxygenic organisms that absorb only 650-690 nm red ight with chlorophyll a. A bacterium Acidiphilium rubrum uses Zn-bacteriochlorophyll a and was shown to undergo highly efficient photo-energy conversion even in the pico-to femto-second time ranges. These new photosynthesis indicated that pigment can be replaced rather easily in photosynthesis although now it is rather rare. On the other hand, the modifications of reaction center proteins yielded drastic decrease of efficiency indicating that the structure of the reaction center is highly optimized for the reaction. Reacion centers are shown to be functional even inside silica mesoscopic nanopores. A part of research to hunt for new organisms was exhibited with arctic plants from Alaska in Aichi World Exposition '06.

  • Research Products

    (15 results)

All 2005 2004

All Journal Article (13 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Primary intermediate in the photocycle of a blue-light sensory BLUF FAD-protein, Tll0078, of Th2005

    • Author(s)
      Fukushima, Y., Okajima, K., Shibata, Y., IK
    • Journal Title

      Biochemistr 44

      Pages: 5149-5158

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of2005

    • Author(s)
      Okajima, K., Yoshihara, S., Fukushima, Y., Geng, X., Katayama, M., Higashi, S., Watanabe, M., Sato, S.
    • Journal Title

      J.Biochem 137(in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Primary intermediate in the photocycle of a blue-light sensory BLUF FAD-protein, T110078,of Thermosynechococcus elongatus BP-1.2005

    • Author(s)
      Fukushima, Y., Okajima, K., Shibata, Y., Ikeuchi, M., Itoh, S.
    • Journal Title

      Biochemistry 44

      Pages: 5149-5158

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria.2005

    • Author(s)
      Okajima, K., Yoshihara, S., Fukushima, Y., Geng, X., Katayama, M., Higashi, S., Watanabe, M., Sato, S., Tabata, S., Shibata, Y., Itoh, S., Ikeuchi, M.
    • Journal Title

      J.Biochem. 137(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nanoscale Organization of Chlorophyll a in Mesoporous Silica : Efficient Energy Transfer and Stabilized Charge Separation as in Natural Photosynthesis2004

    • Author(s)
      Itoh, T., Yano, K., Kajino, T., Itoh.S., Shibata, Y., Mino, H., Inada, Y.
    • Journal Title

      J.Phys.Chem.B 108

      Pages: 13683-13687

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation : Regulation of energy transfer2004

    • Author(s)
      Tsukatani, Y., Miyamoto, R., Itoh.S., Oh-oka, H.
    • Journal Title

      J.Biol.Chem 279

      Pages: 51122-511300

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Phosphatidylglycerol is essential for oligomerization of photosystem I reaction center.2004

    • Author(s)
      Domonkos, I., Malec, P., Sallai, A., Kovacs, L., I
    • Journal Title

      Plant Physiol. 134

      Pages: 1471-1478

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Roles of two ATPase-motif-containing domains in cyanobacerial circadian clock protein KaiC. ansfer and ferredoxin-mediated NADP+reduction on the cytoplasmic side.2004

    • Author(s)
      Hayashi, F., Itoh, N., Uzumaki, T., Iwase, R., Tsuchiya, Y., Yamakawa, H., Morishita, M., Onai
    • Journal Title

      J.Biol.Chem 279

      Pages: 52331-52337

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nanoscale Organization of Chlorophyll a in Mesoporous Silica : Efficient Energy Transfer and Stabilized Charge Separation as in Natural Photosynthesis.2004

    • Author(s)
      Itoh, T., Yano, K., Kajino, T., Itoh, S., Shibata, Y., Mino, H., Inada, Y, Iwai, S., Fukushima, Y.
    • Journal Title

      J.Phys.Chem.B 108

      Pages: 13683-13687

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation ; Regulation of energy transfer and ferredoxin-mediated NADP+ reduction on the cytoplasmic side.2004

    • Author(s)
      Tsukatani, Y., Miyamoto, R., Itoh, S., Oh-oka, H.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 51122-51130

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Phosphatidylglycerol is essential for oligomerization of photosystem I reaction center.2004

    • Author(s)
      Domonkos, I., Malec, P., Sallai, A., Kovacs, L., Itoh, K., Shen, G., Ughy, B., Bogos, B., Sakurai, I., Kis, M., Strzalka, K., Wada, H., Itoh, S., Farkas, T., Gombos, Z.
    • Journal Title

      Plant Physiol. 134

      Pages: 1471-1478

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Roles of two ATPase-motif-containing domains in cyanobacerial circadian clock protein KaiC.2004

    • Author(s)
      Hayashi, F., Itoh, N., Uzumaki, T., Iwase, R., Tsuchiya, Y, Yamakawa, H., Morishita, M., Onai, K., Itoh, S., Ishiura, M.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 52331-52337

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fluorescence of photosysntem I2004

    • Author(s)
      Itoh, S., Sugiura, K.
    • Journal Title

      Chlorophyll fluorescence(ed.by C.Papapgeorgeiou and Govindjee)(Kluwer Academic Publishers, The Netherland) 750

      Pages: 231-250

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Chlorophyll fluorescence : A signature of photosynthesis (2004) (ed. by C.Papapgeorgeiou and Govindiee)2004

    • Author(s)
      Itoh, S., Sugiura.K (共著)
    • Total Pages
      750
    • Publisher
      Kluwer Academic Publishers, The Netherland
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 膜タンパク質複合材料及びその製造方法2004

    • Inventor(s)
      梶野 勉他7名
    • Industrial Property Rights Holder
      名大, トヨタ自, 豊田中研
    • Industrial Property Number
      特願2004-358900
    • Filing Date
      2004-12-10
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2006-07-11  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi