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2005 Fiscal Year Final Research Report Summary

Isolation and identification of the genes inducing bulb formation in bulbous plants

Research Project

Project/Area Number 15380027
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

OKUBO Hiroshi  Kyushu University, Faculty of Agriculture, Professor, 大学院・農学研究院, 教授 (80150506)

Co-Investigator(Kenkyū-buntansha) HIRAMATSU Michikazu  Kyushu University, Faculty of Agriculture, Assistant Professor, 大学院・農学研究院, 助手 (30264104)
OZAKI Yukio  Kyushu University, Faculty of Agriculture, Assistant Professor, 大学院・農学研究院, 助手 (60253514)
INADA Ikuko  Iwate University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90110650)
URESHINO Kenji  Iwate University, Faculty of Agriculture, Lecturer, 農学部, 講師 (10333759)
Project Period (FY) 2003 – 2005
Keywordsbulbous plants / dormancy / genes
Research Abstract

Conditions for controlling bulb formation and non-bulb formation in hyacinth (Hyacinthus orientalis) and lily (Lilium speciosum) were established. Under the bulb forming conditions, eight specific fragments were expressed and nine were suppressed in both species. The DNA sequence of the two fragments, one fragment of 1,000 bp amplified with a primer OPA-08 found in 5C treated and in 25C+ABA treated hyacinth, and another fragment of 500bp amplified with a primer OPA-19 found in 25C+ABA treated lily, showed 98% homology These are considered to be the possible genes that control bulb formation.
Isolation of CIPK3 gene in lily was conducted by degenerative PCR. A fragment of CIPK3 cDNA of 675bp was obtained and it shouwed 65-71% homology with those of six species used for the preparation of degenerate primers.
Two types of Allium scjoenoprasum, one is genetically bulb forming type and the other is genetically non-bulb forming type were used for crossing to obtain F_1 and BC_1 plants. In vitro culture system was established for early identification of bulb formation of the plants. RAPD analysis using 100 random primers was applied to the bulked plants, one for bulb forming group and the other for non bulb forming group that were separated in BC_1 progenies. No specific markers were obtained.

  • Research Products

    (4 results)

All 2005

All Journal Article (4 results)

  • [Journal Article] Characterization of genes related to hyacinth bulb formation induced by low temperature or ABA.2005

    • Author(s)
      Ii-Nagasuga, K., Okubo, H.
    • Journal Title

      Acta Horticulturae 673

      Pages: 575-582

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Search for the genes regulating bulb formation in Lilium speciosum Thunb.2005

    • Author(s)
      Maehara, K., Ii-Nagasuga, K., Okubo, H.
    • Journal Title

      Acta Horticulturae 673

      Pages: 583-589

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In-vitroにおけるAllium schoenoprasum L.実生の球根形成について2005

    • Author(s)
      細矢美穂, 肖靖, 嬉野健次, 稲田委久子, 大久保敬
    • Journal Title

      園芸学会雑誌 74(別2)

      Pages: 195

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In-vitro bulb formation of seedlings in Allium scjoenoprasum L.2005

    • Author(s)
      Hosoya, M., Xiao, J., Ureshino, K., Inada, I., Okubo, H.
    • Journal Title

      Jour.Japan.Soc.Hort.Sci. 74(suppl.2)

      Pages: 195

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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