2005 Fiscal Year Final Research Report Summary
Delivery of foreign proteins into insects
| Project/Area Number |
15380043
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
SUGIMURA Yukio Kyoto Institute of Technology, Graduate School of Science and Technology, Professor, 工芸科学研究科, 教授 (20273542)
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| Co-Investigator(Kenkyū-buntansha) |
FURSAWA Toshiharu Kyoto Institute of Technology, Faculty of Textile Science, Professor, 繊維学部, 教授 (70127166)
NAGAOKA Sumiharu Kyoto Institute of Technology, Faculty of Textile Science, Research Associate, 繊維学部, 助手 (00303933)
KITAJIMA Sakihito Kyoto Institute of Technology, Graduate School of Science and Technology, Research Associate, 工芸科学研究科, 助手 (70283653)
HIRAYAMA Chikara National Institute of Agrobiological Sciences, Chief Researcher, 主任研究官 (90370676)
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| Project Period (FY) |
2003 – 2005
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| Keywords | mulberry plant / silkworm / urease gene / uptake of host protein / transgenic mulberry |
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| Research Abstract |
1. Selective absorption of mulberry urease to the hemolymph of the silkworm : When urease extracted from mulberry roots was applied to the 6^<th> -the 8^<th> day larvae of the 5^<th> instar stage, more than 8% of total injection amount were uptaked into larvae The uptake efficiency was very low in other ureases derived from different organisms. Immunocytological observation shown that urease entry take place via microvilli surface of midgut cells.
2. Identification of mulberry urease gene : Full-length urease cDNA was isolated and the complete nucleotide sequence was determined, and partially defective urease as well. Deducted amino acid sequence (837 residues) from full-length cDNA had a high similarity with those of other plant ureases. The ure G gene coding urease accessory protein G was also isolated and sequenced partially.
3. Gene expression of urease in mulberry seedlings : High urease activity was detected in roots, whereas cotyledons and hypocotyls had no or little activity of urease. In contract, the expression of urease gene was detected in all of the organs. This suggests that additional gene products including ure G protein are involved in maturation of urease protein.
4. Comprehensive detection of protein species uptaked into silkworms : Biotinylated mulberry leaf proteins were applied to the silkworm larvae. The profile by two dimensional electrophoresis shown that 15 biotinylated proteins were uptaked to the larval hemolymph.
5. Experimental system for generating transgenic mulberry plants : A vector plasmid encoding GUS and HTP genes was constructed to transform mulberry plants by a chimeric gene composed of urease gene and foreign gene.
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Research Products
(27 results)
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[Journal Article] Gene expression and localization of trehalase during embryonic development of the silkworm, Bombyx mori.2005
Author(s)
Huang, J., Arimatsu, Y., Sadakane, K., Endo, Y., Furusawa, T.
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Journal Title
Int.J.Wild Silkmoth & Silk 10
Pages: 17-26
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Chemically retted kenaf fibers.2005
Author(s)
Kawahara, Y., Tadokoro, K., Endo, R., Shioya, M., Sugimura, Y., Furusawa, T.
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Journal Title
Sen'i Gakkaishi 61
Pages: 115-117
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Absorption of mulberry root urease to the hemolymph of the silkworm, Bombyx mori.2005
Author(s)
Kurahashi, H., Atiwetin, P., Nagaoka, S., Miyata, S., Kitajima, S., Sugimura, Y.
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Journal Title
J.Insect Physiol. 51
Pages: 1055-1061
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sequence variation in the rbcL-accD region in the chloroplast genome of Moraceae2005
Author(s)
Matsuda, Y., Yoshimura, H., Kanamoto, H., Ujihara, T., Tomizawa, K-I., Sugimura, Y., Kitajima, S.
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Journal Title
Plant Biotech. 22
Pages: 231-233
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transgenic patchouli plants produced by Agrobacterium-mediated transformation.2005
Author(s)
Sugimura, Y., Kadotani, N., Ueda, Y., Shima, K., Kitajima, S., Furusawa, T., Ikegami, M.
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Journal Title
Plant Cell, Tissue and Organ Culture 82
Pages: 251-257
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Induction of genomic DNA fragmentation by introduction of the BmTRN-1 RNA- binding domain into cultured cells of the silkworm, Bombyx mori.2004
Author(s)
Ohba, T., Saito, H., Sugimura, Y., Furusawa, T., Hayashi, Y., Kotani, E.
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Journal Title
J.Insect Biotech.Sericology 73
Pages: 15-22
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] New and highly efficient method for silkworm transgenesis using Autographa californica nucleopolyhedrovirus and piggyBac transposable elements.2004
Author(s)
Yamamoto, M., Yamao, M., Nishiyama, H., Sugihara, S., Nagaoka, S., et al.
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Journal Title
Biotechnol Bioeng. 30
Pages: 849-853
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Metabolic shifts in carbohydrate metabolism during embryonic development of non-diapause eggs of the silkworm, Bombyx mori.2004
Author(s)
Sakano, D., Furusawa, T., Sugimura, Y., Storey, J.M., Storey, K.B.
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Journal Title
J.Insect Biotech.Sericology 73
Pages: 15-22
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] De novo gene expression and antisense inhibition in cultured cells of RmTRN-1, cloned from the midgut of the silkworm, Bombyx mori, which is homologous with mammalian TIA-1/R.2003
Author(s)
Kotani, E., Ohba, T., Niwa, T., Storey, K.B., Storey, J.S., Hara, S., Saito, H., Sugimura, Y., Furusawa, T.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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