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2006 Fiscal Year Final Research Report Summary

Eupyrene-apyrene dimorphic spermatogenesis in Bombyx (Lepidoptera): Significance and differentiation mechanisms.

Research Project

Project/Area Number 15380044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionRakuno Gakuen University

Principal Investigator

YAMASHIKI Naoko  Rakuno Gakuen University, Faculty of Environmental Systems, Professor, 環境システム学部, 教授 (40112578)

Co-Investigator(Kenkyū-buntansha) SAHARA Ken  Hokkaido University, Graduate School of Agriculture, Assistant, 大学院・農学研究科, 助手 (30241368)
Project Period (FY) 2003 – 2006
KeywordsSpermatogenesis / Cell Differentiation / Lepidopteran Insect
Research Abstract

In males of silkworm Bombyx mori, two types of spermatozoa, nucleate eupyrene and anucleate apyrene, are produced in the same testes, although only the eupyrene fertilize the eggs. We focused on that the transition from eupyrene line to the apyrene line occurred in 1) what stages of silkworm development, and 2) what stages of spermatogenesis. It has revealed that 3) not only both types of sperm but the cyst cells, somatic cells that enclose the sperm bundles, follow the different way of development in the testes and the seminal vesicle. 1. The dimorphic sperm production in vitro cultivation of spermatocysts has been almost established, and what stages of silkworm development the transition from eupyrene line to the apyrene line occurred will be cleared by using the cultivation system. 2. By visualizing the chromosome behavior, microtubule dynamics, distribution of y-tubulin (one of the components in microtubule organizing center), any indications of the eupyrene-apyrene line differentiation were not found in the spermatogonia. In the primary spermatocytes, the different fate to eupyrene or apyrene first became evident by the fact that the homologous chromosomes to form the bivalents in eupyrene line and not in apyrene line. Any of molecular candidates were not identified yet in Bombyx. 3. In a layer of cyst cells, differences of histochemical characteristics and submicroscopic structures were detected between the eupyrene and apyrene bundles. After the lysis of cyst cells before they left the testis, apyrene bundles were disrupted while the eupyrene maintained the bundle form. Our findings may indicate that the cyst cells secrete a certain substance to maintain the bundle form of the eupyrene. The invidualized apyrene is considered to play important role in assisting eupyrene bundle during their passage through the male and female genital tracts

  • Research Products

    (12 results)

All 2007 2006 2004 2003

All Journal Article (12 results)

  • [Journal Article] Detection of γ-tubulin in spermatogonial cells of Bobmyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera).2007

    • Author(s)
      Matsuda Y, Sahara K, Yasukochi Y, Yamashiki N
    • Journal Title

      Zool.Sci 24(in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Microtubule dynamics and distribution of γ-tubulin in male germ cells of Bombyx mori (Lepidoptera).2007

    • Author(s)
      Matsuda Y, Yamashiki N
    • Journal Title

      J Insect Biotech Seric. 76(in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression, cellular localization, and functional role of monocarboxylate transporter 4 (MCT4) in the gastrointestinal tract of ruminants.2007

    • Author(s)
      Kirat D, Matsuda Y, Yamashiki N, Hayashi H, Kato S
    • Journal Title

      Gene 391

      Pages: 140-149

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of y-tubulin in spermatogonial cells of Bobmyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera).2007

    • Author(s)
      Matsuda Y, Sahara K, Yasukochi Y, Yamashiki N.
    • Journal Title

      Zool. Sci. 24.(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microtubule dynamics and distribution of γ-tubulin in male germ cells of Bombyx mori(Lepidoptera).2007

    • Author(s)
      Matsuda Y, Yamashiki N.
    • Journal Title

      J Insect Biotech Seric. 76.(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression, cellular localization, and functional role of monocarboxylate transporter 4 (MCT4) in the gastrointestinal tract of ruminants.2007

    • Author(s)
      Kirat D, Matsuda Y, Yamashiki N, Hayashi H, Kato S.
    • Journal Title

      Gene 391

      Pages: 140-149

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori.2006

    • Author(s)
      Takemura Y, Sahara K, Mochida Y, Ohnuma A
    • Journal Title

      J. Insect Physiol. 52

      Pages: 1021-1026

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori.2006

    • Author(s)
      Takemura Y, Sahara K, Mochida Y, OhnumaA
    • Journal Title

      J. Insect Physiol. 52

      Pages: 1021-1026

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Roles of actin networks in peristaltic squeezing of sperm bundles in Bombyx mori2004

    • Author(s)
      Sakara K, Kawamura N
    • Journal Title

      J Morphol. 259(1)

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Roles of actin networks inperistaltic squeezing of sperm bundles in Bombyx mori.2004

    • Author(s)
      Sahara K, Kawamura N
    • Journal Title

      J. Morphol. 259

      Pages: 1-6

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Application of artificial insemination technique to eupyrene and/or apyrene sperm in Bombyx mori.2003

    • Author(s)
      Sahara K, Takemura Y
    • Journal Title

      J Exp Zool Part A Comp Exp Biol. 297(2)

      Pages: 196-200

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Application of artificial insemination technique to separated eupyrene and apyrene sperm in Bombyx mori.2003

    • Author(s)
      Sahara K, Takemura Y
    • Journal Title

      J. Exp. Zool. 297A(2)

      Pages: 196-200

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2008-05-27  

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