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2004 Fiscal Year Final Research Report Summary

MOLECULAR AND CELL-BIOLOGICAL ANALYSIS OF VACUOLAR FUNCTION IN FILAMENTOUS FUNGI

Research Project

Project/Area Number 15380058
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

KITAMOTO Katsuhiko  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (20272437)

Project Period (FY) 2003 – 2004
KeywordsA.oryzae / vacuole / EGFP / A.nidulans / Koji-mold / vps mutants / SNARE / vacuolar membrane
Research Abstract

Vacuoles play very important roles in pH and osmotic regulation, protein degradation and strage of amino acids, small ions as well as polyphosphate in yeast S.cerevisiae. Although it is known that vacuoles in filamentous fungi are highly pleiomorphic and some of them, e.g. tubular vauoles, are implicated in intra-and intercellular transport, little is known about vacuolar functions and morphology dynamics at the molecular level. In this research project I have carried out elucidation of vacuolar function at the molecular and cell-biological level. One of Koji mold A.oryzae vps (Vacular Protein Missorting) mutants, which had been isolated previously, was transformed with cosmid genome library. And the DNA fragment complement the vps mutation was successfully isolated and designated it Aodnf3 due to its homology with yeast DNF3 gene. Next I isolated two genes Aovps10 and Aovam3 from A.oryzae. Expression of EGFP fusion of Aovps10 gene in A.oryzae showed that fluorescence was observed in vacuoles and prevacuolar compartments. In a strain expressing EGFP-Aovam3 fusion, dynamics of vacuolar membranes was clearly observed by confocal laser microscopy. This system enabled to observe vacuole morphology including large spherical vacuoles, tubular vacuoles, and putative prevacuolar compartments at higher resolution level in living cells.

  • Research Products

    (10 results)

All 2005 2004 2003

All Journal Article (9 results) Book (1 results)

  • [Journal Article] Development of Aspergillus oryzae thiA promoter as a tool for molecularbiological studies2005

    • Author(s)
      J.Shoji, J.Maruyama, M.Arioka, K.Kitamoto
    • Journal Title

      FEMS Microbiology Letters 244

      Pages: 41-46

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation and characterization of vacuolar protein sorting mutants from Aspergillus oryzae2005

    • Author(s)
      M.Ohneda, M.Arioka, K.Kitamoto
    • Journal Title

      Appl Environ Microbiol. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation and characterization of vacuolar protein sorting mutants from Aspergillus oryzae2005

    • Author(s)
      M.Ohneda, M.Arioka, K.Kitamoto
    • Journal Title

      Appl.Environ.Microbiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular cloning and functional characterization of avaB, a gene encoding Vam6p/Vps39p-like protein in Aspergillus nidulans2004

    • Author(s)
      M.Oka, J.Maruyama, M.Arioka, H.Nakajima, K.Kitamoto
    • Journal Title

      FEMS Microbiol Letters 232

      Pages: 113-121

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Adenine auxotrophic mutants of Aspergillus oryzae : development of a novel transformation system with triple auxotrophic hosts2004

    • Author(s)
      FJ.Jin, J.Maruyama, PR Juvvadi, M.Arioka, K.Kitamoto
    • Journal Title

      Biosci Biotechnol Biochem 68

      Pages: 656-662

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae2004

    • Author(s)
      FJ.Jin, J.Maruyama, PR Juvvadi, M.Arioka, K.Kitamoto
    • Journal Title

      FEMS Microbiol Letters 239

      Pages: 79-85

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular cloning and functional characterization of avaB, a gene encoding Vam6p/Vps39p-like protein in Aspergillus nidulans2004

    • Author(s)
      M.Oka, J.Maruyama, M.Arioka, H.Nakajima, K.Kitamoto
    • Journal Title

      FEMS Microbial Letters 232

      Pages: 113-121

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae2004

    • Author(s)
      FJ.Jin, J.Maruyama, PR Juvvadi, M.Arioka, K.Kitamoto
    • Journal Title

      FEMS Microbial Letters 239

      Pages: 79-85

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of protein transport inhibitors on the distribution and secretion of the fusion protein the RntA-EGFP in Aspergillus oryzae2004

    • Author(s)
      K.Masai, J.Maruyama, H.Nakajima, K.Kitamoto
    • Journal Title

      Biosci.Biotech.Biochem 68

      Pages: 1569-1573

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 分子麹菌学2003

    • Author(s)
      北本勝ひこ(編著)
    • Total Pages
      144
    • Publisher
      日本醸造協会
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2006-07-11  

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