2005 Fiscal Year Final Research Report Summary
Functional analysis of Escherichia coli RNase G and its application for construction of protein expression system
| Project/Area Number |
15380059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
WACHI Masaaki Tokyo Institute of Technology, Department of Bioscience and Biotechnology, Associate Professor, 大学院・生命理工学研究科, 助教授 (90192822)
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| Project Period (FY) |
2003 – 2005
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| Keywords | RNase G / RNase E / Hfq / FtsZ / stress protection / RNA metabolism / adhE |
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| Research Abstract |
In order to elucidate the regulatory mechanism of mRNA stability by the Escherichia coli endoribonuclease RNase G, action of RNase G on adhE mRNA was investigated. As a result, it was revealed that adhE mRNA cleaved by RNase G at -17 was rapidly degraded while one cleaved by RNase III at -31 was translated. It was found that RNase G mutant strains overproduced pyruvate because of increased stability of mRNAs encoding glycolysis enzymes. According to this finding, a new method for fermentative production of pyruvate and valine was established. It was found that processing of the ftsZ mRNA encoding the essential cell division protein FtsZ was an essential reaction of RNase E required for cell viability. In the RNase E mutant cells, translation of the unprocessed ftsZ mRNA was inhibited by the RNA-binding protein Hfq. For these results, it is suggested that mRNA stability control by endoribonucleases is widely involved in the regulatory mechanism of gene expression in E.coli.
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[Journal Article] Actin homology MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli.2006
Author(s)
Kruse, T., Blagoev, B, Lobner-Olesen, A., Wachi, M., Sasaki, K., Iwai, N., Mann, M., Gerdes, K.
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Journal Title
Genes & Dev. 20
Pages: 113-124
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] FtsZ-dependent localization of GroEL protein at possible division sites.2004
Author(s)
Ogino, H., Wachi, M., Ishii, A., Iwai, N., Nishida, T., Yamasa, S., Nagai, K., Sugai, M.
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Journal Title
Genes Cells 9
Pages: 765-771
Description
「研究成果報告書概要(欧文)」より
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