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2005 Fiscal Year Final Research Report Summary

Functional analysis of Escherichia coli RNase G and its application for construction of protein expression system

Research Project

Project/Area Number 15380059
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

WACHI Masaaki  Tokyo Institute of Technology, Department of Bioscience and Biotechnology, Associate Professor, 大学院・生命理工学研究科, 助教授 (90192822)

Project Period (FY) 2003 – 2005
KeywordsRNase G / RNase E / Hfq / FtsZ / stress protection / RNA metabolism / adhE
Research Abstract

In order to elucidate the regulatory mechanism of mRNA stability by the Escherichia coli endoribonuclease RNase G, action of RNase G on adhE mRNA was investigated. As a result, it was revealed that adhE mRNA cleaved by RNase G at -17 was rapidly degraded while one cleaved by RNase III at -31 was translated. It was found that RNase G mutant strains overproduced pyruvate because of increased stability of mRNAs encoding glycolysis enzymes. According to this finding, a new method for fermentative production of pyruvate and valine was established. It was found that processing of the ftsZ mRNA encoding the essential cell division protein FtsZ was an essential reaction of RNase E required for cell viability. In the RNase E mutant cells, translation of the unprocessed ftsZ mRNA was inhibited by the RNA-binding protein Hfq. For these results, it is suggested that mRNA stability control by endoribonucleases is widely involved in the regulatory mechanism of gene expression in E.coli.

  • Research Products

    (12 results)

All 2006 2005 2004 2003

All Journal Article (12 results)

  • [Journal Article] Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli2006

    • Author(s)
      Kruse, T.
    • Journal Title

      Genes & Dev. 20

      Pages: 113-124

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Actin homology MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli.2006

    • Author(s)
      Kruse, T., Blagoev, B, Lobner-Olesen, A., Wachi, M., Sasaki, K., Iwai, N., Mann, M., Gerdes, K.
    • Journal Title

      Genes & Dev. 20

      Pages: 113-124

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] MreB actin^mediated segregation of a specific region of a bacterial chromosome.2005

    • Author(s)
      Gitai, Z.
    • Journal Title

      Cell 120

      Pages: 329-341

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A decreased level of FtsZ is responsible for inviability of RNase E-deficient cells.2005

    • Author(s)
      Takada, A.
    • Journal Title

      Genes Cells 10

      Pages: 733-741

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] MreB actin-mediated segregation of a specific region of a bacterial chromosome.2005

    • Author(s)
      Gitai, Z., Dye, N.A., Reisenauer, A., Wachi, M., Shapiro, L.
    • Journal Title

      Cell 120

      Pages: 329-341

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A decreased level of FtsZ is responsible for inviability of RNase E-deficient cells.2005

    • Author(s)
      Takada, A., Nagai, K., Wachi, M.
    • Journal Title

      Genes Cells 10

      Pages: 733-741

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] FtsZ-dependent localization of GroEL protein at possible division sites.2004

    • Author(s)
      Ogino, H.
    • Journal Title

      Genes Cells 9

      Pages: 765-771

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] FtsZ-dependent localization of GroEL protein at possible division sites.2004

    • Author(s)
      Ogino, H., Wachi, M., Ishii, A., Iwai, N., Nishida, T., Yamasa, S., Nagai, K., Sugai, M.
    • Journal Title

      Genes Cells 9

      Pages: 765-771

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] RNase ES of Streptomyces coelicolor A3(2) can complement the rne and rng mutations in Escherichia coli.2003

    • Author(s)
      Inagawa, T.
    • Journal Title

      Biosci. Biotechnol. Biochem. 67・11

      Pages: 1767-1771

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Corynebacterium glutamicum rnhA recG double mutant showing lysozyme-sensitivity, temperature-sensitivity growth, and UV-sensitivity.2003

    • Author(s)
      Hirasawa, T.
    • Journal Title

      Biosci. Biotechnol. Biochem. 67・11

      Pages: 2416-2424

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] RNase ES of Streptomyces coelicolor A3(2) can complement the rne and rng mutations in Escherichia coli.2003

    • Author(s)
      Inagawa, T., Okamoto, S., Wachi, M., Ochi, K.
    • Journal Title

      Biosci.Biotechnol.Biochem. 67

      Pages: 1767-1771

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Corynebacterium glutamicum rnhA recG double mutant showing lysozyme-sensitivity, temperature-sensitivity growth, and UV-sensitivity.2003

    • Author(s)
      Hiwasawa, T., Kumagai, Y., Nagai, K., Wachi, M.
    • Journal Title

      Biosci.Biotechnol.Biochem. 67

      Pages: 2416-2424

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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