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2005 Fiscal Year Final Research Report Summary

Clarification of mechanism for antibiotic production regulated by Streptomyces signaling molecules.

Research Project

Project/Area Number 15380063
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionOsaka University

Principal Investigator

NIHIRA Takuya  Osaka University, International Center for Biotechnology, Professor, 生物工学国際交流センター, 教授 (70144441)

Co-Investigator(Kenkyū-buntansha) SEKI Tatsuji  Osaka University, International Center for Biotechnology, Professor, 生物工学国際交流センター, 教授 (50029245)
KINOSHITA Hiroshi  Osaka University, International Center for Biotechnology, Assistant professor, 生物工学国際交流センター, 助手 (20294035)
KAWASAKI Hiroko  Osaka University, International Center for Biotechnology, Assistant professor, 生物工学国際交流センター, 助手 (30251482)
Project Period (FY) 2003 – 2005
KeywordsStreptomyces / Secondary metabolism / Signaling molecules
Research Abstract

Streptomyces species are gram-positive soil-dwelling filamentous bacteria that produce a wide variety of antibiotic and other secondary metabolites with important applications in human medicine and agriculture. These secondary metabolites are products of complex biosynthetic pathways including regulatory genes as well as their biosynthetic gene cluster. The activation of antibiotic production has been known to be controlled by the γ-butyrolactone autoregulator, a low-molecular-weight signaling molecule. Although the autoregulator-mediated mechanism has been partially clarified in many Streptomyces species, common trait has still remained unclear. In this research, we focused on framework for autoregulator-mediated mechanism by assembling information from each analysis. In Streptomyces virginiae, the autoregulator (VB) controls virginiamycin production by means of VB binding to VB receptor (BarA). Our analysis on BarB and BarZ, which possess moderate similarity to BarA, showed that virginiamycin production requires function of BarB and BarZ at initial or stationary phase, respectively. barB transcription is directly controlled by BarA binding to barB promoter region. In S.coelicolor A3(2), the model strain of Streptomyces species, autoregulator receptor was found to regulate directly transcriptional regulator, and thus controls expression of biosynthetic genes concerned with secondary metabolism. Moreover, we obtained 4 more autoregulator receptors from industrially important Streptomyces and non-Streptomyces strains. Taken together with these results, we conclude that useful information was successfully accumulated for industrial application.

  • Research Products

    (15 results)

All 2006 2005 2004 2003 2002

All Journal Article (15 results)

  • [Journal Article] Cloning and characterization of a gene encoding the γ-butyrolactone autoregulator receptor from Saccharopolyspora erythrae.2006

    • Author(s)
      Lee YJ.et al.
    • Journal Title

      J.Microbiol.Biotechnol. 16

      Pages: 77-83

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A bacterial hormone (the SCB1) directly controls the expression of a pathway-specific regulatory gene in the cryptic type I polyketide biosynthetic gene cluster of Streptomyces coelicolor.2005

    • Author(s)
      Takano E.et al.
    • Journal Title

      Mol.Microbiol. 56

      Pages: 465-479

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning and in vivo functional analysis by disruption of a gene encoding the γ-butyrolactone autoregulator receptor from Streptomyces natalensis.2005

    • Author(s)
      Lee KM.et al.
    • Journal Title

      Arch.Microbiol. 184

      Pages: 249-257

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A bacterial hormone (the SCB1) directly controls the expression of a pathway-specific regulatory gene in the cryptic type I polyketide biosynthetic gene cluster of Streptomyces coelicolor.2005

    • Author(s)
      Takano E. et al.
    • Journal Title

      Mol.Microbiol. 56

      Pages: 465-479

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification by gene deletion analysis of barB as a negative regulator controlling an early process of virginiamycin biosynthesis in Streptomyces virginiae.2004

    • Author(s)
      Matsuno K.et al.
    • Journal Title

      Arch.Microbiol. 181

      Pages: 52-59

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin B1 producer.2004

    • Author(s)
      Choi SU.et al.
    • Journal Title

      Arch.Microbiol. 181

      Pages: 294-298

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning and functional analysis by gene disruption of a gene encoding a γ-butyrolactone autoregulator receptor from Kitasatospora setae.2004

    • Author(s)
      Choi SU.et al.
    • Journal Title

      J.Bacteriol. 186

      Pages: 3423-3430

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning and characterization of a gene encoding the γ-butyrolactone autoregulator receptor from Streptomyces clavuligerus.2004

    • Author(s)
      Kim HS.et al.
    • Journal Title

      Arch.Microbiol. 182

      Pages: 44-50

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification by gene deletion analysis of barB as a negative regulator controlling an early process of virginiamycin biosynthesis in Streptomyces virginiae.2004

    • Author(s)
      Matsuno K. et al.
    • Journal Title

      Arch.Microbiol. 181

      Pages: 52-59

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin B1 producer.2004

    • Author(s)
      Choi SU. et al.
    • Journal Title

      Arch.Microbiol. 181

      Pages: 294-298

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning and functional analysis by gene disruption of a gene encoding a γ-butyrolactone autoregulator receptor from Kitasatospora setae.2004

    • Author(s)
      Choi SU. et al.
    • Journal Title

      J.Bacteriol. 186

      Pages: 3423-3430

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] γ-butyrolactone autoregulators and receptor proteins in non-Streptomyces actinomycetes producing commercially important secondary metabolites.2003

    • Author(s)
      Choi SU.et al.
    • Journal Title

      Arch.Microbiol. 180

      Pages: 203-307

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] γ-butyrolactone autoregulators and receptor proteins in non-Streptomyces actinomycetes producing commercially important secondary metabolites.2003

    • Author(s)
      Choi SU. et al.
    • Journal Title

      Arch.Microbiol. 180

      Pages: 303-307

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] barS1, a gene for biosynthesis of a γ-butyrolactone autoregulator, a microbial signaling molecule eliciting antibiotic production in Streptomyces species.2002

    • Author(s)
      Shikura N.et al.
    • Journal Title

      J.Bacteriol. 184

      Pages: 5151-5157

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] barS1, a gene for biosynthesis of a γ-butyrolactone autoregulator, a microbial signaling molecule eliciting antibiotic production in Streptomyces species.2002

    • Author(s)
      Shikura N. et al.
    • Journal Title

      J.Bacteriol. 184

      Pages: 5151-5157

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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