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2005 Fiscal Year Final Research Report Summary

Structure and Electron Transfer of Quinoproteins and application to Bio-electrode Devices

Research Project

Project/Area Number 15380082
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionKyoto University

Principal Investigator

KANO Kenji  Kyoto Univ., Grad.Sch.Agric., Prof., 農学研究科, 教授 (10152828)

Project Period (FY) 2003 – 2005
KeywordsHistamine dehydrogenase / 6-S-cyteinyl-FMN / Expression system / Quinohemoproteins / Amine dehydrogenase / Alcohol dehydrogenase / Biosensors / Biofuel cells
Research Abstract

1)Structural analysis and Electron Transfer Characteristics of Histamine Dehydrogenase (HmDH)
It has been revealed that HmDH has 6-S-cyteinyl-FMN and 4Fe-4S cluster as redox prosthetic groups. Under neutral conditions, 2-electron reductions occurs per monomer by the substrate, while the number of the electron is switched to unit over pH 9.5. The properties can be explained in terms of the change in the redox potentials. The kinetics of half-reduction has been analyzed with stopped flow method. The holo form of HmDH has been expressed in E.coli in the purity of 100%. Site-directed mutation improve the specificity of the enzyme.
2)Electron Transfer of Quinohemoproteins
Positive charge in the vicinity of the active site of quinohemoprotein amine dehydrogenase (QH-AmDH) plays an important role in the electron transfer from to electron acceptor. The direct electron transfer from QH-AmDH to electrodes was not observed. The redox potentials of quinohemoproteins alcohol dehydrogenase depend on pH. This would be due to the structural change of the enzyme.
3)Application to Biosensor and Biofuel Cells
It has been found that mutant HmDH works as a good catalysis of mediated biosensor for histamine with high specificity. Selectivity of mediator reduced the interference. The dehydrogenases invested here were utilized also as catalysts in bioanode of biofuel cells, where bilirubin oxidase (BOD) was used as cathode enzyme. Direct electron transfer of BOD is affected by the electrode materials. The current density were increased up few ten mA cm^<-2>.

  • Research Products

    (14 results)

All 2006 2005

All Journal Article (13 results) Book (1 results)

  • [Journal Article] Novel FAD-dependent Glucose Dehydrogenase to Construct Dioxygen-insensitive Glucose Biosensor2006

    • Author(s)
      Seiya Tsujimura
    • Journal Title

      Biosci. Biotech. Biochem. 70(3)

      Pages: 2459-2462

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Novel FAD-dependent Glucose Dehydrogenase to Construct Dioxygen-insensitive Glucose Biosensor2006

    • Author(s)
      Seiya Tsujimura et al.
    • Journal Title

      Biosci.Biotech.Biochem. 70(3)

      Pages: 2459-2462

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Production of Completely Flavinylated Histamine Dehydrogenase, Unique Covalently Bound Flavin and Iron-sulfur Cluster Containing Enzyme of Nocardioides simplex in Escherichia coli, and its Properties2005

    • Author(s)
      Nobutaka Fujieda
    • Journal Title

      Biosci. Biotech. Biochem. 69(12)

      Pages: 2459-2462

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation Process of 〔NiFe〕 Hydrogenase Elucidated by High Resolution X-ray Analyses : Conversion of the Ready to Unready State2005

    • Author(s)
      Hideaki Ogata
    • Journal Title

      Structure 13(11)

      Pages: 1635-1642

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mediated Spectroelectrochemical Titration of Proteins for Redox Potential Measurements by a Separator-less One-compartment Bulk Electrolysis Method2005

    • Author(s)
      Seiya Tsujimura
    • Journal Title

      Anal. Biochem. 337(2)

      Pages: 325-331

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Electron Transfer Reactions of Glucose Oxidase at Au(111) Electrodes Modified with Phenothiazine Derivatives2005

    • Author(s)
      Sayaka Nanjo
    • Journal Title

      Anal. Chem. 77(13)

      Pages: 4142-4147

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Bilirubin Oxidase in Multiple Layer Catalyzes Four-electron Reduction of Dioxygen to Water without Redox Mediators2005

    • Author(s)
      Seiya Tsujimura
    • Journal Title

      J. Electroanal. Chem. 576(1)

      Pages: 113-125

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Production of Completely Flavinylated Histamine Dehydrogenase, Unique Covalently Bound Flavin and Iron-sulfur Cluster Containing Enzyme of Nocardioides simplex in Escherichia coli, and its Properties2005

    • Author(s)
      Nobutaka Fujieda, et al.
    • Journal Title

      Biosci.Biotech.Biochem. 69(12)

      Pages: 2459-2462

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Activation Process of [NiFe] Hydrogenase Elucidated by High Resolution X-ray Analyses : Conversion of the Ready to Unready State2005

    • Author(s)
      Hideaki Ogata et al.
    • Journal Title

      Structure 13(11)

      Pages: 1635-1642

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mediated Spectroelectrochemical Titration of Proteins for Redox Potential Measurements by a Separator-less One-compartment Bulk Electrolysis Method2005

    • Author(s)
      Seiya Tsujimura et al.
    • Journal Title

      Anal.Biochem. 337(2)

      Pages: 325-331

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Electron Transfer Reactions of Glucose Oxidase at Au(111) Electrodes Modified with Phenothiazine Derivatives2005

    • Author(s)
      Sayaka Nanjo et al.
    • Journal Title

      Anal.Chem. 77(13)

      Pages: 4142-4147

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bilirubin Oxidase in Multiple Layer Catalyzes Four-electron Reduction of Dioxygen to Water without Redox Mediators2005

    • Author(s)
      Seiya Tsujimura et al.
    • Journal Title

      J.Electroanal.Chem. 576(1)

      Pages: 113-125

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Current Status of Batteries in Next Generation2005

    • Author(s)
      Seiya Tsujimura et al.
    • Journal Title

      E.T.S.

      Pages: 620

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 電池革新が拓く次世代電源事情2005

    • Author(s)
      辻村清也
    • Total Pages
      620
    • Publisher
      エス・ティー・エス
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

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