2005 Fiscal Year Final Research Report Summary
Gene expression analysis related to regulation of life cycle using Porphyra genomic information
| Project/Area Number |
15380126
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAGA Naotsune Hokkaido Univ., Fac.of Fis., Prof., 大学院・水産科学研究院, 教授 (10231333)
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| Co-Investigator(Kenkyū-buntansha) |
YASUI Hajime Hokkaido Univ., Fac.of Fis., Asso.Prof., 大学院・水産科学研究院, 助教授 (00200494)
MIZUTA Hiroyuki Hokkaido Univ., Fac.of Fis., Asso.Prof., 大学院・水産科学研究院, 助教授 (00250499)
KITADE Yukihiro Hokkaido Univ., Fac.of Fis., Contributing Lecturer, 大学院・水産科学研究院, 寄附講座教員 (90399999)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Porphyra yezoensis / Porphyra genome / EST / life cycle / monospore / allantoin |
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| Research Abstract |
New findings obtained by this study are as follows.
1. Screening of highly expressed genes during asexual sporulation through monospores
We found eight genes encoding enzymes related to metabolism and unknown genes with gametophytic generation-phase-specific expression pattern by cDNA macroarray and RT-PCR or Northern analysis. The eight nucleotide sequences will appear in the GenBank/EMBL/DDBJ databases with accession numbers AB127044 through AB127051.
2. Development of a new technique for gene expression analysis and internal standard in Porphyra yezoensis
(1) We developed a method for whole-mount in situ hybridization (WISH) by using elongation factor-1α (EF-1α) riboprobes and gametophytic germlings.
(2) We developed an internal standard (actin-related protein 4 homologue) in gene expression analyses of differentially expressed genes. A putative bipartite nuclear localization signal (NLS) and an actin motif were found within the PyARP4 amino acid sequences. The expression level of PyARP4 did not change significantly among four developmental stages of the life cycle and was lower than that of a conventional actin.
3. Induction of monospore production by chemical treatment
We found that monospore-like cells with germinability can be obtained when vegetative gametophytic thalli of P.yezoensis were grown in enriched seawater medium that contained 10 mM allantoin. We also found that this chemical treatment is effective to even gametophytic thalli of P.pseudolinearis, which does not have asexual sporulation through monospores in the life cycle.
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