2005 Fiscal Year Final Research Report Summary
Molecular diet analysis of larvae of important marine animals
| Project/Area Number |
15380137
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Incorporated Administrative Agency Fisheries Research Agency |
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Principal Investigator |
ICHII Taro National Research Institute of Far Seas Fisheries, Oceanic Resources Division, Section Chief, 遠洋水産研究所・外洋資源部, 研究室長 (50360871)
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| Co-Investigator(Kenkyū-buntansha) |
CHOW Seinen National Research Institute of Fisheries Science, Coastal Fisheries and Aquaculture Division, Section Chief, 中欧水産研究所・浅海増殖部, 研究室長 (70360766)
MOCHIOKA Noritaka Kyushu University, Faculty of Agriculture, Assistant Professor, 大学院・農学研究院, 助手 (40212261)
SAKAI Mitsuo National Research Institute of Far Seas Fisheries, Oceanic Resources Division, Senior Researcher, 遠洋水産研究所・外洋資源部, 主任研究員 (70371937)
YOSHIMURA Taku Seikai National Fisheries Research Institute, Coastal Fisheries and Aquachulture Division, Section Chief, 西海区水産研究所・海区水産業研究部, 研究室長 (90371997)
YAMADA Harumi National Research Institute of Far Seas Fisheries, Western Pacific Tuna and Skipjack Resources Division, Section Chief, 遠洋水産研究所・温帯性まぐろ資源部, 研究室長 (10371915)
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| Project Period (FY) |
2003 – 2005
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| Keywords | spiny lobster / eel / squid / tuna / larvae / stomach content / genome typing |
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| Research Abstract |
Since species identification of the host larvae is important to investigate the stomach content, we have developed molecular markers to determine species of spiny lobsters, cephalopods, lantern fishes and eels. Especially, identification of squid larvae significantly contributed to stock assessment and dynamics of squid and fishes utilizing squids. Phylogenetic analysis of tuna species was performed using novel nuclear DNA. Larvae of neon squid, eel, conger eel, scyllarid lobsters and palinurid lobsters were used for molecular diet analysis. In all larvae, fungi and host variant 18S rDNA were detected, and no candidate organism as prey was found in the squid and eels. On the other hand, 18S rDNA of cnidaria and urochordata were found in two (Panulirus longipes bispinosus and P.echinatus) of three palinurid species and one (Scyllarides sp.) of two scyllarid lobster species. Fish DNA was found in the Japanese spiny lobster (P.japonicus).
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Research Products
(27 results)
