2005 Fiscal Year Final Research Report Summary
Generation of bioactive peptides from meat proteins during aging and intestinal digestion
| Project/Area Number |
15380187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Kitasato University |
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Principal Investigator |
ARIHARA Keizo Kitasato Univ., Dept.of Anim.Sci., Associate Prof., 獣医畜産学部, 助教授 (00175994)
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| Project Period (FY) |
2003 – 2005
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| Keywords | meat / protein / aging / protease / bioactive peptide / antioxidative activity / functional property / prebiotics |
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| Research Abstract |
This research project has been conducted to investigate the generation of bioactive peptides from meat proteins by proteases during meat aging and intestinal digestion. Since our studies showed an increase in bioactivities (e.g., ACE inhibitory activity) of beef and pork products during aging or strage, the generation of bioactive peptides was onfirmed to occur by aging or storage. Many bioacitive peptides (e.g., antihypertensive peptides, antioxidative peptides, prebiotic peptides, opioide peptides) derived from meat proteins have been identified in this study. For example, several novel antioxidative peptides generated from pork proteins were discovered. Of these antioxidative peptides, the peptide, which showed the highest activity, was Asp-Leu-Tyr-Ala. This peptide also showed some remarkable activities (e.g., anti-fatigue activity) in vivo. Since the mixture of four individual amino acids did not show the biological activity, the tetra peptide-structure of Asp-Leu-Tyr-Ala is critical for its activity. By the treatment of digestive tract enzymes, Asp-Leu-Tyr-Ala generated tripeptide (Leu-Tr-Ala). Leu-Try-Ala showed similar activities with Asp-Leu-Tyr-Ala both in vitro and in vivo experiments. In another experiment, a novel bioactive peptide, Glu-Leu-Met was identified in the pork protein digest. Glu-Leu-Met is the first prebiotic (Bifidobacterium growth-promoting) peptide generated from food proteins by enzymatic digestion. Since this peptide is highly resistant to digestive enzymes, it would be a promising prebiotic component. As bioactive peptides would be liberated from food proteins during gastrointestinal proteolysis, further studies are now in progress to identify the generation of such peptides from meat proteins in the intestinal tracts.
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[Journal Article] Functional foods.2004
Author(s)
Arihara, K.
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Journal Title
Encyclopedia of Meat Sciences.(W.K.Jensen, C.Devine, M.Dikeman (Eds.))(Oxford : Elsevier)
Pages: 245-274
Description
「研究成果報告書概要(欧文)」より
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