2004 Fiscal Year Final Research Report Summary
Supplemental lactoferrin improves health of Holstein neonate calves
| Project/Area Number |
15380197
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | National Agriculture and Bio-oriented Research Organization |
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Principal Investigator |
KUSHIBIKI Shiro National Institute of Livestock and Glassland Science, Department of Animal Physiology and Nutrition, 畜産草地研究所・家畜生理栄養部, 室長 (30355218)
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| Co-Investigator(Kenkyū-buntansha) |
SHINGU Hiroyuki National Agricultural Research Center for Tohoku Region, Department of Animal Production and Glasslands Farming, 東北農業研究センター・畜産草地部, 主任研究官 (40355219)
OBARA Yoshiaki Tohoku University, Faculty of Agriculture, Graduate School of Agricultural Science, 大学院・農学研究科, 教授 (50302196)
ITOH Fumiaki National Institute of Livestock and Glassland Science, Department of Animal Physiology and Nutrition, 畜産草地研究所・家畜生理栄養部, 主任研究官 (60391380)
KOMATSU Tokushi National Institute of Livestock and Glassland Science, Department of Animal Physiology and Nutrition, 畜産草地研究所・家畜生理栄養部, 研究員 (90360453)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Lactoferrin / Cattle / Acute phase response / metabolites / cytokine |
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| Research Abstract |
Lactoferrin (Lf) is an iron-binding protein present in colostrums, saliva, and intestinal mucos. The protein has a role in iron absorption and is an important antimicrobial component of the host defence. We hypothesized that supplemental Lf improves acute inflammation in endotoxin shock in neonatal calves. Newborn calves (10 d of age) were assigned to receive saline, Lf (200 mg/kg), or Lf (400 mg/kg). The calves were fed saline or Lf at 0900 and 2100 h. At 0900 h on next day, the calves received an i.v. injection lipopolysaccharide (50 ng/kg, E.coli 055 ; Shigma Chemical Co., St.Louis, MO) as endotoxin. In the calves treated with saline, plasma concentrations of ferrum, retinol, and lipoprotein fraction (VLDL and LDL) were decreased between 24 and 96 h after endotoxin administration. In the calves treated with Lf, these metabolites were not decreased by endotoxin injection. In addition, Lf feeding inhibited fever and production of haptpglobin, tumor necrosis factor-alpha (TNF), interleukin-6 (IL-6) following endotoxin treatment in calves. In summary, we have demonstrated that oral treatment of calves with Lf diminished the intensity of endotoxin-induced acute inflammation which was associated with significantly decreased acute phase protein and pro-inflammatory cytokines such as TNF and IL-6. These findings are in accordance with other results on anti-inflammatory properties of Lf in various in vitro models.
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