2005 Fiscal Year Final Research Report Summary
Roles of Glial Cells for Neurodegeneration in Prion Diseases
| Project/Area Number |
15380202
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
NAKAMURA Yoichi Osaka Prefecture University, Grad.School of Life and Environmental Sciences, Lab. of Integrative Physiology in Veterinary Science, Professor, 生命環境学研究科, 教授 (90180413)
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| Co-Investigator(Kenkyū-buntansha) |
MORIYAMA Mitsuaki Osaka Prefecture University, Grad.School of Agriculture and Biological Sciences, Lab. of Integrative Physiology in Vet.Science, Associate Professor, 生命環境学研究科, 助教授 (20275283)
FUJIMOTO Yuka Osaka Prefecture University, Grad.School of Agriculture and Biological Sciences, Lab. of Integrative Physiology in Vet.Science, Assistant Professor, 生命環境学研究科, 助手 (40405361)
KANNAN Yukiko Osaka Prefecture University, Grad.School of Agriculture and Biological Sciences, Lab. of Integrative Physiology in Vet.Science, Guest Investigator, 生命環境学研究科, 客員研究員 (80264810)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Microglia / Astrocyte / EC-SOD / Amphotericin B / NOS / NO production |
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| Research Abstract |
It is unknown how affects Amphotericin B (AmB), reported to be a therapeutic drug against prion diseases, on microglial function. Cultured microglia prepared from rat new-born brain were stimulated to produce nitric oxide (NO) by prion peptide (106-126) (PrP), and also by AmB. The level of the NO production stimulated by AmB was enhanced by the presence of the PrP. These results suggest a certain interaction between AmB and PrP and the interaction modulates the microglial cell function. The elucidation of this interaction might be a key for the therapeutic mechanism of AmB against prion diseases.
The physiological functions of prion protein is speculative; however, it is suggested that prion protein is involves in the, mechanism of Cu transport, which is an essential metal for superoxide dismutase (SOD), through cell membrane. We have established the method for the measurement of extracellular SOD (EC-SOD) activity in the living cells. The activities of EC-SOD of cultured astrocytes from rat embryo's brain decreased to a half after stimulation by lipopolysaccharide (LPS) for 24 hr, whereas the intracellular SODs (cytoplasmic and mitochondrial) increased. On the other hand, the activity of SOD in the culture medium increased by LPS dose-dependently. These results suggest that EC-SOD is released from cell surface into the medium. Astrocytes may play an important role for the regulation of brain parenchymal level of active oxygen species by changing localization of EC-SOD ; protecting surrounding neurons from oxidative stress.
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Research Products
(19 results)
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[Journal Article] Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes.2006
Author(s)
Nakamura, Y., Kitagawa, T., Ihara, H., Kozaki, S., Moriyama, M., Kannan.Y
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Journal Title
Neurochem Int. 48(1)
Pages: 43-49
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes.2006
Author(s)
Nakamura, Y., Kitagawa, T., Ihara, H., Kozaki, S., Moriyama, M., Kannan, Y
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Journal Title
Neurochem Int. 48(1)
Pages: 43-49
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Apparent presence of Serl33-phosphorylated cyclic AMP response element binding protein (pCREB) in brain mitochondria is due to cross-reactivity of pCREB antibodies with pyruvate dehydrogenase.2005
Author(s)
Platenik, Jan., Balcar V.J., Yoneda, Y., Kuramoto, N.Wilczynski, G., Ogita, K., Nakamura, Y., Kaczmarek, L
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Journal Title
Neurochem. 95(5)
Pages: 1446-1460
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Apparent presence of Ser133-phosphorylated cyclic AMP response element binding protein (pCREB) in brain mitochondria is due to cross-reactivity of pCREB antibodies with pyruvate dehydrogenase.2005
Author(s)
Platenik, Jan., Balcar V.J., Yoneda, Y.,...Kuramoto, N., Wilczynski, G., Ogita, K., Nakamura, Y., Kaczmarek, L.
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Journal Title
J Neurochem. 95(5)
Pages: 1446-1460
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neuroprotective effects of ONO-1924H, an inhibitor of poly ADP-ribose polymerase (PARP), on cytotoxicity of PC12 cell and ischemic cerebral damage.2004
Author(s)
Kamanaka, Y., Kondo, K., Suzuki, Y., Nakamura, Y., Umemura, K.
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Journal Title
Life Sciences 76
Pages: 151-162
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Neuroprotective effects of ONO-1924H, an inhibitor of poly ADP-ribose polymerase (PARP), on cytotoxicity of PC12 cell s and ischemic cerebral damage.2004
Author(s)
Kamanaka, Y., Kondo, K., Suzuki, Y., Nakamura, Y., Umemura, K.
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Journal Title
Life Sciences 76
Pages: 151-162
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Adenosine triphosphate inhibits cytokine release freom lipopolysaccharide-activated microglia via P2y receptors.2003
Author(s)
Ogata, T., Chuai, M., Marina, T., Yamamoto, H., Nakamura, Y., Schubert, P.
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Journal Title
Brain Research 981
Pages: 174-183
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Adenosine triphosphate inhibits cytokine release from lipopolysaccharide-activated microglia via P2y receptors.2003
Author(s)
Ogata, T., Chuai, M., Morino, T., Yamamoto, H., Nakamura, Y., Schubert, P.
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Journal Title
Brain Research 981
Pages: 174-183
Description
「研究成果報告書概要(欧文)」より
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