2005 Fiscal Year Final Research Report Summary
Integration and visualization of genome information in Lepidoptera
| Project/Area Number |
15380227
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | HOKKAIDO UNIVERISTY |
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Principal Investigator |
SAHARA Ken Hokkaido Univ., Grad.School of Agr., Assistant Prof., 大学院・農学研究科, 助手 (30241368)
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| Co-Investigator(Kenkyū-buntansha) |
YASUKOCHI Yuji National Inst. Agrobiological Sciences, Senior Researcher, ゲノム研究グループ, 主任研究官 (50355723)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Bombyx mori / BAC-FISH / GISH / contig / linkage map / chromosome identification / karyotyping / Lepidoptera |
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| Research Abstract |
We have developed Bombyx mori sex chromosome identification method and first BAC-FISH system in Lepidoptera by means of W-BAC selected by female specific STSs in 2003. The former method has been applied to resolve multiple sex chromosome systems in 2005. Modified BAC-FISH procedure using genetically mapped B.mori BACs as probes allow us to identify each 28 bivalents. Furthermore, the combination of two to four either green or red fluorescence-labeled probes per chromosome enables us to recognize unequivocally each of the 28 bivalents of the B.mori karyotype by its labeling pattern, which have been impossible for more than 80 years. Through the research we have accomplished 2nd generation molecular linkage map including 512 BAC contigs, which correspond to most unique genes published on the web. These may feasibly facilitate the map-based cloning the genes corresponding to mutants.
The BAC-FISH signal localities coincide with their mapping loci detected by genetic analysis with a few exceptions. Since the discrepancy may be caused by mistakes of three-point analysis, we had better re-calculate the results.
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Research Products
(30 results)
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[Journal Article] Simple sequence repeat-based consensus linkage map of Bombyx mori.2005
Author(s)
Miao XX, Xub SJ, Li MH, Li MW, Huang JH, Dai FY, Marino SW, Mills DR, Zeng P, Mita K, Jia SH, Zhang Y, Liu WB, Xiang H, Guo QH, Xu AY, Kong XY, Lin HX, Shi YZ, Lu G, Zhang X, Huang W, Yasukochi Y, Sugasaki T, Shimada T, Nagaraju J, Xiang ZH, Wang SY, Goldsmith MR, Lu C, Zhao GP, Huang YP
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Journal Title
Proc.Natl.Acad.Sci.USA 102
Pages: 16303-16308
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Partial deletions of the W chromosome due to reciprocal translocation in the silkworm Bombyx mori.2005
Author(s)
Abe H, Seki M, Ohbayashi F, Tanaka N, Yamashita J, Fujii T, Yokoyama T, Takahashi M, Banno Y, Sahara K, Yoshido A, Ihara J, Yasukochi Y, Mita K, Ajimura M, Suzuki MG, Oshiki T, Shimada T
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Journal Title
Insect Mol.Biol. 14
Pages: 339-352
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The Genome Sequence of Silkworm, Bombyx mori.2004
Author(s)
Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki N, Kitagawa M, Yamashita H, Yasukochi Y, Kadono-Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin-I T, Abe H, Shimoda T, Morishita, Sasaki S
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori.2003
Author(s)
Sahara K, Yoshido A, Kawamura N, Ohnuma A, Abe H, Mita K, Oshiki T, Shimoda T, Asano S, Bando H, Yasukochi Y
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Journal Title
Chromosoma 112
Pages: 48-55
Description
「研究成果報告書概要(欧文)」より
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