2005 Fiscal Year Final Research Report Summary
Immune-neuro Cross-talk with Confocal Laser Scanning Microscopy
| Project/Area Number |
15390017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
NAKANISHI Mamoru Nagoya City University, Graduate School of Pharmaceutical Sciences, Professor, 大学院・薬学研究科, 教授 (90090472)
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| Co-Investigator(Kenkyū-buntansha) |
HIRASHIMA Naohide Nagoya City University, Graduate School of Pharmaceutical Sciences, Associate Professor, 大学院・薬学研究科, 助教授 (10192296)
FURUNO Tadahide Aichi Gakuin University, School of Pharmacy, Associate Professor, 薬学部, 助教授 (80254308)
TESHIMA Reiko National Institute of Health Sciences of Japan, Principal Investigator, 機能生化学部, 室長 (50132882)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Mast cell / SCG / Laser microscopy / Substance P / N-cadherin / SynCAM / ATP / RBL |
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| Research Abstract |
Communication between nerves and mast cells is a prototypic demonstration of neuro-immune interaction. Previously, we showed clearly that nerve-mast cell cross-talk can occur bidirectionally in the absence of an intermediary transducing cell using an in vitro co-culture approach and calcium imaging. In terms of bidirectional communication, we demonstrated that the neuropeptide substance P is an important mediator in efferent communication (i.e. neuron-to-mast cell), but the mediators in afferent communication (i.e. mast cell-to-neuron) have been still unknown. Then, we have studied the mediators to activate neurites (superior cervical ganglia ; SCG) cocultured with mast cells (rat basophilic leukemia cells ; RBLs). Addition of antigen to the cocultures of SCG and RBLs elicited the increase in the [Ca^<2+>]_i in RBLs and after a lag period induced the increase in the [Ca^<2+>]_i in SCG neuretes extending at a long distance (>150μm) from the site where the RBLs attached to the neurites. The pretreatment of pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS) or apyrase, which is a purinergic receptor 2 antagonist or an ATP hydrolyzing enzyme, respectively, prevented the calcium signals in SCG neuritis which were resulted as a consequence of RBL activation, but the pretreatment of chlorophenilamine or ketanserin, which is a histamine or serotonin receptor antagonist, respectively, did not. These results indicated that the ATP released from activated mast cells functioned as an important mediator in afferent communication between neurites and mast cells, but not histamine and serotonin. In addition, we found the roles of SynCAM and N-cadherin in the synaps-like structures between mast cells and neuritis.
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[Journal Article] The spermatogenic Ig superfamily/synaptic cell adhesion molecule mast-cell adhesion moleculepromotes interaction with nerves.2005
Author(s)
Furuno, T., Ito, A., Bienenstock, J., Nakanishi, M., Kitamura, Y.
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Journal Title
J. Immunol., 174
Pages: 6934-6942
Description
「研究成果報告書概要(和文)」より
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[Journal Article] The spermatogenic Ig superfamily/synaptic cell adhesion molecule mast-cell adhesion molecule promotes interaction with nerves.2005
Author(s)
Furuno, T., Ito, A., Koma, Y., Watabe, K., Yokozaki, H., Bienenstock, J., Nakanishi, M., Kitamura, Y.
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Journal Title
J.Immunol. 174
Pages: 6934-6942
Description
「研究成果報告書概要(欧文)」より
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[Book] 物理系薬学III2006
Author(s)
中西 守 他
Total Pages
1-213
Publisher
東京化学同人(共著)
Description
「研究成果報告書概要(和文)」より
