Study of prenatal exposure to diesel exhaust on male reproductive and central nerve systems of mice

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 15390042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Tokyo University of Science
• Principal Investigator: TAKEDA Ken Tokyo University of Science, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (80054013)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Seiichi Oita University of Nursing and Health Sciences, Assistant Professor, 講師 (40360060)
• Project Period (FY): 2003 – 2005
• Keywords: diesel exhaust / in utero exposure / central nerve system / male reproductive system / accessory reproductive gland / testosterone / multi-nuclear giant cells / spontaneous motor activity

Research Abstract

The effects of in utero exposure to diesel exhaust (DE) on the mouse male reproductive system and central nerve system were examined. Various markers that appear in pups were assessed. Pregnant ICR mice inhaled DE at DEP concentrations of 0.3, 1.0, or 3.0 mg DEP/m3 or clean air, as the control, on days 2-16 days post coitum. On postnatal day (PND) 28, the weight of the testes and accessory glands, and testosterone concentration in serum were significantly higher in the DE-exposed male pups. Testosterone concentration correlated significantly (P<0.01) with the expression of steroidogenic enzyme mRNAs, weight of the testes and male reproductive accessory glands, and daily sperm production. A spontaneous motor activity was changed intentionally, and the change of the dopamine, noradrenalin and the serotonin systems was admitted in the brain.
These findings indicate that early stage mouse embryo exposure to DE affects male genital system and the cranial nerve system after birth.

Research Products

• [Journal Article] Prenatal exposure to diesel exhaust impairs mouse spermatogenesis. (2007)
  • Author(s): Naoka Ono et al.
  • Journal Title: Inhalation Toxicology 19 Pages: 275-281.
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] (2006) Prenatal exposure to diesel exhaust impairs mouse spermatogenesis. (2007)
  • Author(s): Ono N, Oshio S, Niwata Y, Yoshida S, Tsukue N, Sugawara I, Takano H, Takeda K
  • Journal Title: Inhalat Toxicol 19 Pages: 275-281
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In utero exposure to diesel exhaust increased accessory reproductive gland weight and serum testosterone concentration in male mice. (2006)
  • Author(s): Seiichi Yoshida et al.
  • Journal Title: Environmental Science 13 Pages: 139-147.
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mice Strain Differences in effects of fetal exposure to diesel exhaust gas on male gonadal differentiation. (2006)
  • Author(s): Seiichi Yoshida et al.
  • Journal Title: Environmental Science 13 Pages: 117-123
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In utero exposure to diesel exhaust increased accessory reproductive gland weight and serum testosterone concentration in male mice. (2006)
  • Author(s): Yoshida S, Ono N, Tsukue N, Oshio S, Umeda T, Takano H, Takeda, K
  • Journal Title: Environ Sci 13 Pages: 139-147
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mice Strain Differences in effects of fetal exposure to diesel exhaust gas on male gonadal differentiation. (2006)
  • Author(s): Yoshida S, Yoshida M, Sugawara I, Takeda, K
  • Journal Title: Environ Sci 13 Pages: 117-123
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Diesel exhaust affects immunological action in the placenta of mice. (2005)
  • Author(s): Ayaha Fujimoto et al.
  • Journal Title: Environ Toxicol 20 Pages: 431-440
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Diesel exhaust affects immunological action in the placenta of mice (2005)
  • Author(s): Fujimoto A, Tsukue N, Watanabe M, Sugawara I, Yanagisawa R, Takano H, Yoshida S, Takeda, K
  • Journal Title: Environ Toxicol 20 Pages: 431-440
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Endocrine-disrupting activity of chemicals in diesel exhaust and diesel exhaust particles (2004)
  • Author(s): Ken Takeda et al.
  • Journal Title: Environmental Science 11(1) Pages: 33-45
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of diesel exhaust on development of fuel reproductive function in ICR female mice (2004)
  • Author(s): Naomi Tsukue et al.
  • Journal Title: Journal of Health Science 50(2) Pages: 174-180
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Endocrine-disrupting activity of chemicals in diesel exhaust and diesel exhaust particles. (2004)
  • Author(s): Takeda K, Tsukue N, Yoshida S
  • Journal Title: Environ Sci 11 Pages: 33-45
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of diesel exhaust on development of fetal reproductive function in ICR female mice. (2004)
  • Author(s): Tsukue N, Yoshida S, Sugawara I, Takeda K
  • Journal Title: J Health Sci. 50 Pages: 174-180
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 科学 (2006)
  • Author(s): 武田 健 他
  • Total Pages: 6
  • Publisher: 岩波書店
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 化学と教育 (2003)
  • Author(s): 机 直美, 他
  • Total Pages: 2
  • Publisher: 日本化学会
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 科学 (2003)
  • Author(s): 吉田 成一
  • Total Pages: 2
  • Publisher: 岩波書店
  • Description: 「研究成果報告書概要(和文)」より