2004 Fiscal Year Final Research Report Summary
Identification of membrane proteins participating myogenesis
| Project/Area Number |
15390089
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SEHARA Atsuko Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (60209038)
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| Co-Investigator(Kenkyū-buntansha) |
KURISAKI Tomohiro Kyoto University, Institute for Frontier Medical Sciences, Assistant, 再生医科学研究所, 助手 (90311422)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Myogenesis / cellular differentiation / si RNA / GFP / membrane proteins |
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| Research Abstract |
Coordinated development of multi-cellular organisms depends on intercellular communications and adhesions. Our research has been focused on identification of membrane proteins participating such cell-cell interactions during myogenesis. Inititially, we made a cDNA library of mRNA prepared from fusing muscle cells, which was subtracted with mRNA of myoblasts. From the screening of this library, we could identify a couple of novel membrane proteins. We are now trying to know functions of these proteins in myogenesis. We also succeeded to isolate a number of monoclonal antibodies from fusing muscle cells. Finally we studied roles of ADAM proteases in myogenesis, which we had cloned as membrane proteins activated by myogenic transcription factors. Based on the analyses of Meltrin α -/- and Meltrin β -/- mice, we concluded that these ADAM proteins indeed participate in myogenesis in vivo.
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