2004 Fiscal Year Final Research Report Summary
Identification of human mitochondrial metabolome proteins
| Project/Area Number |
15390106
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KANG Dongchon Kyushu University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究院, 助教授 (80214716)
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| Project Period (FY) |
2003 – 2004
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| Keywords | mitochondria / mitochondrial DNA / nucleoid / mass spectrometery / mitochondrial transcription factor A / TFAM / RNAi |
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| Research Abstract |
(Aim) Mitochondrial DNA (mtDNA) encodes genes essential for the mitochondrial electron transfer system. Recently we proposed that mtDNA takes on a large protein-DNA complex called nucleoid and the nucleoid structure is critical for maintenance of mtDNA. Mitochondrial transcription factor A (TFAM) is a main component of the nucleoid. In this study, I aim to identify protein components of the nucleoid by LC/MS/MS.
(Methods) Isolated human mitochondria were treated with a c ross-linker. After solubilization, the nucleoid was immunoprecipitated with anti-TEAM antibody. The precipitated proteins were separated on one dimensional SDS-PAGE, extracted, tripsinized, and and analyzed by LC/MS/MS.
(Results)
1)Potential DNA- or RNA binding proteins
2)Heat shock proteins
3)Ribosomal proteins
4)TCA cycle related proteins
5)Enzymes for β oxidation of fatty acids
All proteins identified were classified into only 5 groups as above. These results suggest that the mitochondrial nucleoid provides a scaffold for efficient metabolism of translation, TCA cycles, and β oxidation of fatty acids.
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[Journal Article] Architectural role of TFAM in maintenance of human mitochondrial DNA.2004
Author(s)
Kanki, T., Ohgaki, K., Gaspari, M., Gustafsson, C.M., Fukuoh, A., Sasaki, N., Hamasaki, N., Kang, D.
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Journal Title
Mol.Cell.Biol. 24
Pages: 9823-9834
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mitochondrial nucleoid and transcription factor A.2004
Author(s)
Kanki, T., Nakayama, H., Sasaki, N., Takio, K., Alam, T.I., Hamasaki, N., Kang, D.
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Journal Title
Ann N Y Acad Sci 1011
Pages: 61-68
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32.2004
Author(s)
Sunayama, J., Ando, Y, Itoh, N., Tomiyama, A, Sakurada, K., Sugiyama, A., Kang, D., Tashiro, F., Gotoh, Y, Kuchino, Y, Kitanaka, C.
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Journal Title
Cell Death Differ 11
Pages: 771-781
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] High-Sensitivity Detection of the A3243G Mutation of Mitochondrial DNA by a Combination of Allele-Specific PCR and Peptide Nucleic Acid-Directed PCR Clamping.2004
Author(s)
Urata, M., Wada, Y., Kim, S., Chumpia, W., Kayamori, Y., Hamasaki, N., Kang, D.
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Journal Title
Clin Chem 50
Pages: 2045-2051
Description
「研究成果報告書概要(欧文)」より
