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2005 Fiscal Year Final Research Report Summary

Analysis of height-determining gene.

Research Project

Project/Area Number 15390324
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionOsaka University

Principal Investigator

OZONO Keiichi  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (20270770)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Shigeo  Osaka University, Graduate School of Medicine, Associate professor, 医学系研究科, 助教授 (30270771)
ETANI Yuri  Osaka University, Graduate School of Medicine, Instructor, 医学系研究科, 助手 (80346220)
Project Period (FY) 2003 – 2005
Keywordsshort stature / overgrowth / gene trap / PTPN11 / skeletal dysplasia / progeria / glucocorticoid / SHOX
Research Abstract

We attempted to identify genes which are involved in proliferation and differentiation of chondrocytes because it is likely that such genes affect height. In addition, we examined phenotype and genotype correlation in diseases characterized with short stature. First, we applied a gene-trap approach in the murine mesenchymal cell line ATDC5, and obtained many trap clones with impaired differentiation. Among them, trapped genes were identified to encode p85alpha, subunit of phosphatidylinositide 3-kinase (PI3K) and nuclear factor I-B (NFIB), a transcriptional factor. Trapped clones in which functions of PI3K or NFIB were impaired showed abnormal differentiation of chondrocytes. These result suggest that these genes are candidates of height-determining genes. Hypochondroplasia is caused by a mutation of the fibroblast growth factor receptor 3 (FGFR3) gene and show phenotype milder than achondroplasia. A mutation of the gene, N540K, was found in 3 patients with hypochondroplasia. Gene analysis of FGFR3 is useful to identify hypochondroplasia, leading to the treatment of patients with growth hormone (GH). Compound heterozygous mutation was found in the ZMPSTHE24 gene in sibling cases of mandibuloacral dysplasia. Eight mutations were found in the PTPN11 gene in patients with Noonan syndrome. We analyzed the function of SHP-2 encoded by PTPN11 and obtained results which suggest the correlation between the activity of SHP-2 and the degree of short stature. ESCO2 was firstly identified as a gene responsible for Roberts syndrome which showed very short extremities. The polymorphism of the cAMP-response element binding protein-binding protein (CBP) gene was correlated to susceptibility of glucocorticoid in patients with osteonecrosis.

  • Research Products

    (12 results)

All 2005 2004 2003

All Journal Article (12 results)

  • [Journal Article] Roberts syndrome is caused by mutations in ESCO2, a human homolog of yeast ECO1 that is essential for the establishment of sister chromatid cohesion.2005

    • Author(s)
      Vega H, et al.
    • Journal Title

      Nature Genet. 37・5

      Pages: 468-470

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel mutaion in the PTPN11 gene in a patient with Noonan syndrome and rapidly progressive hypertrophic cardiomyopathy.2005

    • Author(s)
      Takahashi K, et al.
    • Journal Title

      Eur J Pediatr. 164・8

      Pages: 497-500

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Longitudinal observation of a patients with Leri-Weill dyschondrosteosis and SHOX haploinsufficiency.2005

    • Author(s)
      Miyoshi Y, et al.
    • Journal Title

      Clin Pediatr Endocrinol. 14・1

      Pages: 11-16

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Robert syndrome is caused by mutations in ESCO2, a human homolog of yeast ECO1 that is essential for the establishment of sister chromatid cohesion.2005

    • Author(s)
      Vega H, et al.
    • Journal Title

      Nature Genet 37(5)

      Pages: 468-470

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel mutation in the PTPN11 gene in a patients with Noonan syndrome and rapidly progressive hypertrophic cardiomyopathy.2005

    • Author(s)
      Takahashi K, et al.
    • Journal Title

      Eur J Pediatr 164(8)

      Pages: 497-500

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Longitudinal observation of a patients with Leri-Weill dyschondrosteosis and SHOX haploinsufficiency.2005

    • Author(s)
      Miyoshi Y, et al.
    • Journal Title

      Clin Pediatr Endocrinol 14(1)

      Pages: 11-16

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involvement of phosphoinositide 3-kinase signaling pathway in chondrocytic differentiation of ATDC5 cells : application of a gene-trap mutagenesis.2004

    • Author(s)
      Ihara-Watanabe M, et al.
    • Journal Title

      J Cell Biochem. 93・2

      Pages: 418-426

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hormonal and genetical assessment of a Japanese girl with Weaver syndrome.2004

    • Author(s)
      Miyoshi Y, et al.
    • Journal Title

      Clin Pediatr Endocrinol. 13・1

      Pages: 17-23

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Involvement of phosphoinositide 3-kinase signaling pathway in chondrocytic differentiation of ATDC5 cells : application of a gene-trap mutagenesis.2004

    • Author(s)
      Ihara-Watanabe M, et al.
    • Journal Title

      J Cell Biochem 93(2)

      Pages: 418-426

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hormonal and genetical assessment of a Japanese girl with Weaver syndrome.2004

    • Author(s)
      Miyoshi Y, et al.
    • Journal Title

      Clin Pediatr Endocrinol 13(1)

      Pages: 17-23

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A patient with Sotos syndrome who reached final height.2003

    • Author(s)
      Miyoshi Y, et al.
    • Journal Title

      Clin Pediatr Endocrinol. 12・20

      Pages: 37-40

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A patient with Sotos syndrome who reached final height.2003

    • Author(s)
      Miyoshi Y, et al.
    • Journal Title

      Clin Pediatr Endocrinol 12(20)

      Pages: 37-40

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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