2005 Fiscal Year Final Research Report Summary
New support system of vascular surgery using novel bio-materials
| Project/Area Number |
15390373
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOYAMA Hiroyuki The University of Tokyo, Faculty of Medicine, Visiting Associate Professor, 医学部附属病院, 寄附講座教員 (10241994)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Tetsuro The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (70190791)
NISHIYAMA Nobuhiro The University of Tokyo, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (10372385)
KATAOKA Kazunori The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00130245)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Acidic gelatin hydrogel / Polymer nano-micellsb / Reverse transfection / Intimal Hyperplasia / Rabbit carotid artery / Therapeutic angiogenesis / bFGF / RGD |
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| Research Abstract |
(1) Development of new therapeutic angiogenesis using novel bio-materials
The present study utilized acidic gelatin hydrogel microspheres (AGHM) and polymer nano-micelle particle system (NP) as novel bio-material. In AGHM, recombinant bFGF protein was bound to AGHM, and the bFGF-impregnated AGHM was administrated through targeted artery in rabbit model of chronic limb ischemia. Twenty eight days after the injection, several assessments showed significant improvement of blood perfusion and neovascularization in the ischemic limb. Meanwhile in NP, the complex with marker gene DNA was first injected to muscle tissue, and gene transfer efficiency was evaluated. However, no significant expression of marker gene was detected. To improve the efficacy of gene transfer, we added ligand peptides (RGD peptide, ring-formed RGD peptide or artery wall binding peptide) to the NP (RGD-NP, rRGD-NP and AWBP-NP). Though, these ligand-added NPs showed no significant improvement of gene transfer efficiency to muscle tissue. Therefore, we studied new gene transfer approach of NP by using reverse transfection method, and obtained favorable result under in vitro condition.
(2) Gene transfer to intimal hyperplasia using novel bio-materials
To develop gene transfer method to intimal hyperplasia of vessels, we applied NP complexed with marker gene DNA to neointimal layer of rabbit carotid artery. Although non-ligand NP, RGD-NP, rRGD-NP and AWBP-NP were examined in this study, no significant increase of gene transfer was detected as compared with control. Then, we also carried out the evaluation of new poly-ion micelle, PEG-DET, and observed favorable gene delivery to neointima of rabbit carotid artery.
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Research Products
(10 results)
