• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2004 Fiscal Year Final Research Report Summary

Establishment of melecular-based diagnositics for esophageal cancer

Research Project

Project/Area Number 15390398
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MORI Masaki  Kyushu University, Medical Institute of Bioregulation, Professor, 生体防御医学研究所, 教授 (70190999)

Co-Investigator(Kenkyū-buntansha) INOUE Hiroshi  Kyushu University, Medical institute of Bioregulation, Associate Professor, 生体防御医学研究所, 助教授 (90203249)
UTSUNOMIYA Tohru  Kyushu University, Kyushu Univ. Hospital, Assistant Professor, 大学病院, 講師 (30304801)
MIMORI Koshi  Kyushu University, Medical institute of Bioregulation, Research Associate, 生体防御医学研究所, 助手 (50322748)
Project Period (FY) 2003 – 2004
Keywordsmicrometastasis / esohageal cancer / chemical carcinogenesis / radiation sensitivity / MAL / MMP2 / FHIT / CKS2
Research Abstract

Esophageal cancer is considered to be one of the most aggressive malignacy in the Eastern countries, including Japan. Early detection of the tumor development as well as tumor recurences has been a key subject of the disease. The purpose of our current study is to establish molecular-based diagnositics for esophageal cancer and we have conveyed 4 major researches for this purpose : 1) the detection of the tumor cells (micrometastasis) in either blood or bone marrow of the patient for the postoperative disease surveillance, 2) the establishment of rat esophageal chemical-induced carcinoma model for the elucidation of the genes associated with esophageal carcinogenesis, 3) the detection of the genes responsible for radiation sensitivities, 4) the detection of tumor associated genes using DNA microarray methodology applied for the human esophageal tumor specimens. Results : 1) The blood and bone marrow samples were obtained from 150 patients with esophageal cancer. No significant differences were currently observed between the cases with early recurrence or metastasis of the tumor. More detailed-studies are needed for longer period of the postoperative observation. 2) Genes such as rat gelatinase A (MMP2), keratin 6a, Lyn B tyrosine kinase were found to be highly expressed in development of carcinoma. Genes such as ALDH. SLP were repressed along the tumor development. 3) Genes such as SPP1, CKS2 were found to be associated with tumor radio sensitivity. 4) Transfection of MAL and FHIT genes induce dramatic changes in tumor cell biology. For the gene FHIT, the mechanism of apoptotic activity by Ad-FHIT is to identify the candidate cancer associated targets affected by Ad-FHIT treatment. Those molecules may consider to be both tumor markers for the early detection of tumor development, and also a fine molecular target for the treatments of esophagel cancer.

  • Research Products

    (12 results)

All 2005 2004

All Journal Article (12 results)

  • [Journal Article] Global Analysis of Altered Gene Expressions during the Process of Esophageal Squamous Cell Carcinogenesis in the Rat : A Study Combined with a Laser Microdi ssection and a cDNA Microarray. Cancer2005

    • Author(s)
      Nishida K. et al.
    • Journal Title

      Cancer Res 65

      Pages: 401-409

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Familial cancer associated with a polymorphism in ARLTS12005

    • Author(s)
      Calim, G.A., et al.
    • Journal Title

      N Engl J Med 26

      Pages: 41-47

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Somatic mutations of epidermal growth factor receptor in colorectal carcinoma.2005

    • Author(s)
      Nagahara H et al.
    • Journal Title

      Clin Cancer Res 11

      Pages: 1368-1371

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of occult cancer cells in peripheral blood and bone marrow by quantitative RT-PCR assay for cytokeratin-7 in breast cancer patients.2005

    • Author(s)
      Masuda, T.A., et al.
    • Journal Title

      Int J Oncol 26

      Pages: 721-730

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Global analysis of altered gene expressions during the process of esophageal squamous cell carcinogenesis in the rat : a study combined with a laser microdissection and a cDNA microarray.2005

    • Author(s)
      Nishida, K., Mine, S., Utsunomiya, T., Inoue, H., Okamoto, M., Udagawa, H., Hanai, T., Mori M.
    • Journal Title

      Cancer Res 65

      Pages: 401-409

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Familial cancer associated with a polymorphism in ARLTS1.2005

    • Author(s)
      Calin, G.A., Trapasso, F., Shimizu, M., Dumitru, C.D., Yendamuri, S., Godwin, A.K., Ferracin, M., Bernardi, G., Chatterjee, D., Baldassarre, G., Rattan, S., Alder, H., Mabuchi, H., Shiraishi, T., Hansen, L.L., Overgaard, J., Herlea, V., Mauro, F.R., Dighiero, G., Movsas, B., Rassenti, L., Kipps, T., Baffa, R., Fusco, A., Mori, M., Russo, G., Liu, C.G., Neuberg, D., Bullrich, F., Negrini, M., Croce, C.M.
    • Journal Title

      N Eng1 J Med 26

      Pages: 41-47

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Somatic mutations of epidermal growth factor receptor in colorectal carcinoma.2005

    • Author(s)
      Nagahara, H., Mimori, K., Ohta, M., Utsunomiya, T., Inoue, H., BARNARD, G.F., Ohira, M., Hirakawa K., Mori, M.
    • Journal Title

      Clin Cancer Res 11

      Pages: 1368-1371

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection of occult cancer cells in peripheral blood and bone marrow by quantitative RT-PCR assay for cytokeratin-7 in breast cancer patients.2005

    • Author(s)
      Masuda, T.A., Kataoka, A., Ohno, S., Murakami, S., Mimori, K., Utsunomiya, T., Inoue, H., Tsutsui, S., Kinoshita, J., Masuda, N., Moriyama, N., Mori, M.
    • Journal Title

      Int J Oncol 26

      Pages: 721-730

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma.2004

    • Author(s)
      Yoshinaga, K., et al.
    • Journal Title

      Clin Cancer Res 10

      Pages: 5702-5707

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma.2004

    • Author(s)
      Ogawa, K et al.
    • Journal Title

      Br J Cancer 91

      Pages: 282-286

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma.2004

    • Author(s)
      Yoshinaga, K., Inoue, H., Utsunomiya, T., Sonoda, H., Masuda, T., Mimori, K., Tanaka, Y., Mori, M.
    • Journal Title

      Clin Cancer Res 10

      Pages: 5702-5707

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma.2004

    • Author(s)
      Ogawa, K., Utsunomiya, T., Mimori, K., Tanaka, Y., Tanaka, F., Inoue, H., Murayama, S., Mori, M.
    • Journal Title

      Br J Cancer 91

      Pages: 282-286

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2006-07-11  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi