2005 Fiscal Year Final Research Report Summary
Development of Undifferentiated Embryonic Stem Cell- incorporated Small Caliber Vascular Prostheses
| Project/Area Number |
15390421
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | KYOTO PREFECTURAL UNIVERSITY OF MEDICINE |
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Principal Investigator |
KANDA Keiichi Kyoto Prefectural University of Medicine, Cardiovascular Surgery, Lecturer, 医学研究科, 講師 (60295649)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Yasuhide National Cardiovascular Center Research Institute, Department of Bioengineering, Director, 生体工学部, 室長 (50250262)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Embryonic Stem Cells / Hybrid Vascular Prostheses / Mouse / Autologous Connective Tissue Tube / Mechanical Stress |
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| Research Abstract |
Embryonic stem cells (ES cells) are expected as alternative cell resources for autologous cell-incorporated hybrid artificial organs. However, there have been very little information about ES cell behaviors in vivo. Vascular wall cells in vivo are continuously exposed to biomechanical stimulations, such as hydrodynamic sheer stress, periodical stretching and pulsatile pressure. Therefore it is very important to know the behaviors of ES cells under such conditions when application of the cells to circulatory systems is considered.
To investigate the behavior of the ES cells under hemodynamic conditions in vivo, we developed the systematic animal experimental model of ES cell-incorporated hybrid vascular prostheses in this study.
ES cells derived from a fetal mouse were randomly differentiated on the Type IV collagen-coated culture plates in vitro to induce Flk-1+ cells. Next, the cells before and after cell sorting were seeded on the luminal surface of polyurethane vascular prostheses wit
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h a diameter of 1.5mm. After incubation with VEGF for predetermined days, the grafts were implanted to the aortas of nude rats for up to 7 days with the use of specially designed connecting devices. Implanted grafts were examined morphologically and immunologically.
After implantation, incorporated ES cells retained on the surface of the grafts under the exposure to pulsatile blood flow. Since ES cells were raveled with Lac-Z gene, it was possible to determine that the observed cells were derived from incorporated ES cells. Observed cells were polygonal shaped and did not yet exhibit the typical aspects of the vascular wall cells in this series (=<7 days). However, some of the incorporated ES cells, exhibited PECAM-1, which indicated that further in vivo differentiation of the ES cells was induced under bio-mechanical and bio-chemical stimulations.
This ES cells-incorporated hybrid vascular prosthesis is the first model for investigation of the behavior of ES cells under hemodynamic conditions in vivo, and might provide important information for clinical application of ES cell-incorporated cardiovascular artificial organs. Less
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[Journal Article] In vitro pulsatile flow loading can induce the differentiation from embryonic stem (ES) cells to vascular wall cells2005
Author(s)
Haiying Huang, Yasuhide Nakayama, Kairong Qin, Kimiko Yamamoto, Joji Ando, Jun Yamashita, Itoh Hiroshi, Keiichi Kanda, Hitoshi Yaku, Yoshihiro Okamoto, Yasushi Nemoto
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Journal Title
J.Artificial Organs 8(2)
Pages: 110-118
Description
「研究成果報告書概要(和文)」より
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[Journal Article] バイオチューブ人工血管におけるES細胞の生体内分化誘導2005
Author(s)
坂井修, 神田圭一, 中山泰秀, 黄海瑛, 岡本吉弘, 根本泰, 山下潤, 伊藤裕, 安藤穣二, 渡辺太治, 夜久均
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Journal Title
日本血管外科学会雑誌 14(3)
Pages: 502
Description
「研究成果報告書概要(和文)」より
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[Journal Article] In vitro pulsatile flow loading can induce the differentiation from embryonic stem (ES) cells to vascular wall cells.2005
Author(s)
Huang H, Nakayama Y, Qin K, Yamamoto K, Ando J, Yamashita J, Itoh H, Kanda K, Yaku H, Okamoto Y, Nemoto Y.
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Journal Title
Artificial Organs Sep;29(9)
Pages: 733.3
Description
「研究成果報告書概要(和文)」より
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[Journal Article] ES Cell-Differentiation to the Vascular Wall Cells Induced by Mechanical Stimulations in vitro.2005
Author(s)
Koh K, Nishida M, Nakayama Y, Yamamoto K, Ando J, Nakayama Y, Yamashita J, Itoh H, Kanda K, Okamoto Y, Nemoto Y, Yaku H, Nakayama Y.
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Journal Title
Jpn.J.Regenerative Medicine. Vol.4 Supp.
Pages: 184
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vitro pulsatile flow loading can induce the differentiation from embryonic stem (ES) cells to vascular wall cells.2005
Author(s)
Haiying Huang, Yasuhide Nakayama, Kairong Qin, Kimiko Yamamoto, Joji Ando, Jun Yamashita, Itoh Hiroshi, Keiichi Kanda, Hitoshi Yaku, Yoshihiro Okamoto, Yasushi Nemoto
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Journal Title
J.Artificial Organs. 8(2)
Pages: 110-118
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Differentiation of ES Cells Incorporated in Biotube Vascular Grafts in vivo.2005
Author(s)
Sakai O, Kanda K, Nakayama Y, Koh K, Okamoto Y, Nemoto Y, Yamashita J, Itoh H, Ando J, Watanabe T, Yaku H.
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Journal Title
Jpn.J.Vasc.Surg. Vol.14(3)
Pages: 502
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vitro pulsatile flow loading can differentiate embryonic stem (ES) cells to vascular wall cells.2005
Author(s)
H Huang, Y Nakayama, K Qin, K Yamamoto, J Ando, J Yamashita, H Itoh, K Kanda, H Yaku, Y Okamoto, Y Nemoto.
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Journal Title
Artificial Organs 2005 27(9)
Pages: 779.1
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] ハイブリッド型人工血管の開発をめざす、in vitro拍動流負荷によるES細胞の血管壁構成細胞への力学的分化誘導2004
Author(s)
黄海瑛, 西田真美, 中山泰亮, 山本希美子, 安藤穣二, 山下潤, 伊藤裕, 神田圭一, 岡本吉弘, 根本泰, 夜久均
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Journal Title
日本人工臓器学会雑誌 Vol.32-2
Pages: S171
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Aim for Development of Hybrid Vascular Prosthesis - ES Cell-Differentiation to the Vascular Wall Cells under Pulsatile Flow in vitro.2004
Author(s)
Koh K, Nishida M, Nakayama Y, Yamamoto K, Ando J, Nakayama Y, Yamashita J, Itoh H, Kanda K, Okamoto Y, Nemoto Y, Yaku H.
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Journal Title
Jpn.J Artif.Organ. Vol.32(2)
Pages: S171
Description
「研究成果報告書概要(欧文)」より
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[Patent(Industrial Property Rights)] 細胞分化誘導法2005
Inventor(s)
中山泰秀, 黄海瑛, 安藤穣二, 山下潤, 伊藤裕, 神田圭一, 岡本吉弘, 根本泰
Industrial Property Rights Holder
国立循環器病センター総長, 株式会社ブリヂストン
Industrial Property Number
特願2005-066683
Filing Date
2005-03-10
Description
「研究成果報告書概要(和文)」より
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[Patent(Industrial Property Rights)] 人工弁2005
Inventor(s)
中山泰秀, 神田圭一, 根本 泰
Industrial Property Rights Holder
国立循環器病センター総長, 株式会社ブリヂストン
Industrial Property Number
特願2005-225099
Filing Date
2005-08-03
Description
「研究成果報告書概要(和文)」より
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