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2006 Fiscal Year Final Research Report Summary

Identification of a candidate gene associated with paclitaxel-resistance and application to a gene therapy in ovarian cancer.

Research Project

Project/Area Number 15390509
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKYUSHU UNIVERCITY

Principal Investigator

OGAWA Shinji  Kyushu University, Hospital, Research Associate, 大学病院, 助手 (60380391)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Hiraoaki  Kyushu University, Hospital, Research Associate, 大学病院, 助手 (70260700)
SONODA Kenzo  Kyushu University, Hospital, Research Associate, 大学病院, 助手 (30294929)
UEOKA Yousuke  Kyushu University, Hospital, Research Associate, 大学病院, 助手 (50372743)
矢幡 秀昭  九州大学, 大学病院, 助手 (30404065)
WAKE Norio  Kyushu University, Faculty of Medical Sciences, Professor, 医学研究院, 教授 (50158606)
Project Period (FY) 2003 – 2006
Keywordspaclitaxel resistance / ovarian cancer / cDNA microarray / β-tubulin
Research Abstract

We established an in vivo paclitaxel-resistant cell line from the parental human ovarian cancer cell lines by repeated paclitaxel administration into tumor-bearing mice. We performed cDNA microarrays between in vivo established paclitaxel-resistant cell line and in vivo established control group to identify the target genes associated with paclitaxel-resistancy. Total RNAs were extracted (1) from in vivo established paclitaxel-resistant cell lines and control group, and (2) from tumor blocks (OM1TX6T and OM1P6T)which were made by subcutaneous transplantion of paclitaxel-resistant cells or control cells.
cDNA were purified and cancer-related gene manifestation profiles which were specifically increased or decreased between two groups were identified. Genes which we used for analysis (1) were 9,063, and the number of the genes which expressed in a resistant cell lines more than double of control cell lines. In addition, genes which we used for analysis (2) were 8,660,and similarly the number of the genes which expressed OM1TX6T more than double of OM1P6T was 298. Now we really push forward extraction of genes related to paclitaxel-resistance from these genes.
On the other hand, overexpression of class III B-tubulin was attracted attention as mechanism of taxane-resistance. We evaluated protein expression level of class I, II, II, IV (IVa+IVb) isotypes of B-tubulin in an ovarian cancer specimens by immunohistochemical stainig, and examined the association with responce to taxane-containing chemotherapy. As a result, in the goup which had chemotherapy with taxane, high expression of class III isotypes of B-tubulin tend to correlate with short prograssion free survival (p=0.081), while this tendency was not found in the group which had chemotherapy without taxane.

  • Research Products

    (4 results)

All 2007 2004

All Journal Article (4 results)

  • [Journal Article] Expression of beta-tubulin isotypes in human primary ovarian carcinoma2007

    • Author(s)
      Ohishi Y
    • Journal Title

      Gynecol Oncol Mar 5

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of beta-tubulin isotypes in human primary ovarian carcinoma2007

    • Author(s)
      Ohishi Y
    • Journal Title

      Gynecol Oncol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vivo-establishment and characterization of a paclitaxel- resistant human ovarian cancer cell line showing enhanced growth properties and drug-resistance only in vivo.2004

    • Author(s)
      Okugawa K
    • Journal Title

      J Cancer Res Clin Oncol 130

      Pages: 178-186

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vivo-establishment and characterization of a paclitaxel-resistant human ovarian cancer cell line showing enhance growth properties and drug-resistance only in vivo.2004

    • Author(s)
      Okugawa K
    • Journal Title

      J Cancer Res Clin Oncol. 130

      Pages: 178-186

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2008-05-27  

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