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2005 Fiscal Year Final Research Report Summary

Production of clinically applicable tissue-engineered cornea

Research Project

Project/Area Number 15390525
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionThe University of Tokyo

Principal Investigator

AMANO Shiro  The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (80193027)

Co-Investigator(Kenkyū-buntansha) YAMAGAMI Satoru  The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (10220245)
USUI Tomohiko  The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (80282557)
TANABE Tatsuro  The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (00359605)
Project Period (FY) 2003 – 2005
KeywordsCornea / Regenerative medicine / Corneal epithelium / Corneal endothelium
Research Abstract

To produce clinically applicable tissue-engineered cornea, it is required to obtain tissue stem cells from each of three layers of the cornea. First, we showed that tissue stem cells can be obtained from each of three layers of the cornea by neuro-sphere assay. Then, we investigated the method of applying these tissue stem cells in animal models. We injected spheres from human corneal endothelium into the anterior chamber of rabbits and the spheres attached to the back surface of rabbit cornea, maintaining the transparency of the cornea. To evaluate the feasibility of a method of human corneal endothelial cells (HCEC) sheet transplantation using collagen membrane of 40 μm as a substitute carrier of Descemet's membrane. These results provide evidence for the feasibility of cultured HCEC sheet transplantation using collagen membrane for corneal endothelial decompensation. We seeded spheres from human corneal stroma onto the porous gelatin sheets of 40-50μm and transplanted the sheet into the corneal stromal pockets of rabbits. Then, the spheres from human corneal stroma produced abundant of extracellular matrix such as laminin and fibronectin, indicative of the clinical feasibility of spheres from human corneal stroma. We compared the distribution and self-renewal capacity of rabbit corneal endothelial cell precursors in the central and peripheral regions of the cornea. Our findings demonstrate that both peripheral and central rabbit corneal endothelium contain a significant number of precursors, but the peripheral endothelium contains more precursors with a stronger self-renewal capacity relative to the central region.

  • Research Products

    (12 results)

All 2005

All Journal Article (12 results)

  • [Journal Article] Long-term outcome of iron-endocytosing cultured corneal endothelial cell transplantation with magnetic attraction.2005

    • Author(s)
      Mimura T(Amano S)
    • Journal Title

      Experimental Eye Research 80

      Pages: 149-157

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sphere formation and expression of neural proteins by human corneal stromal cell in vitro.2005

    • Author(s)
      Uchida S(Amano S)
    • Journal Title

      Investigative Ophtalmology and Visual Science 46

      Pages: 1620-1625

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Human corneal endothelial cell precursors isolated by sphere-forming assay2005

    • Author(s)
      Yooko S(Amano S)
    • Journal Title

      Investigative Ophtalmology and Visual Science 46

      Pages: 1626-1631

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sphere therapy for corneal endothelium deficiency in a rabbit model2005

    • Author(s)
      Mimura T(Amano S)
    • Journal Title

      Investigative Ophthalmology and Visual Science 462

      Pages: 3128-3135

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Treatment of rabbit bullous keratopathy with precursors derived from cultured human corneal endothelium.2005

    • Author(s)
      Mimura T(Amano S)
    • Journal Title

      Investigative Ophthalmology and Visual Science 46

      Pages: 3637-3644

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Comparison of rabbit corneal endothelial cell precursors in the central and peripheral cornea2005

    • Author(s)
      Mimura T(Amano S)
    • Journal Title

      Investigative Ophthalmology and Visual Science 46

      Pages: 3645-3648

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Long-term outcome of iron-endocytosing cultured corneal endothelial cell transplantation with magnetic attraction.2005

    • Author(s)
      Mimura T, (Amano S).
    • Journal Title

      Experimental Eye Research 80

      Pages: 149-157

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sphere formation and expression of neural proteins by human corneal stromal cell in vitro2005

    • Author(s)
      Uchida S, (Amano S).
    • Journal Title

      Invest Ophthalmol Vis Sci 46

      Pages: 1620-1625

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human corneal endothelial cell precursors isolated by sphere-forming assay2005

    • Author(s)
      Yokoo S, (Amano S).
    • Journal Title

      Invest Ophthalmol Vis Sci 46

      Pages: 1626-1631

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sphere therapy for corneal endothelium deficiency in a rabbit model2005

    • Author(s)
      Mimura T, (Amano S).
    • Journal Title

      Invest Ophthalmol Vis Sci 46

      Pages: 3128-3135

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Treatment of rabbit bullous keratopathy with precursors derived from cultured human corneal endothelium.2005

    • Author(s)
      Mimura T, (Amano S).
    • Journal Title

      Invest Ophthalmol Vis Sci 46

      Pages: 3637-3644

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Comparison of rabbit corneal endothelial cell precursors in the central and peripheral cornea2005

    • Author(s)
      Mimura T, (Amano S).
    • Journal Title

      Invest Ophthalmol Vis Sci 46

      Pages: 3645-3648

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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