2005 Fiscal Year Final Research Report Summary
Development of tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes
| Project/Area Number |
15390530
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
NISHIDA Koji Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (40244610)
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| Co-Investigator(Kenkyū-buntansha) |
OKANO Teruo Tokyo Women's Medical University, Professor, 先端生命医科学研究所, 教授 (00130237)
TANO Yasuo Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (80093433)
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| Project Period (FY) |
2003 – 2005
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| Keywords | corneal epithelium / corneal endothelium / regenerative medicine / temperature-responsive culture dish / SP cell / gene transfer / tissue engineering / stem cell |
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| Research Abstract |
We investigated a tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes. In this project, we obtained the following results. Regarding the project for development of corneal epithelial cell sheet transplantation, (1) we have developed a technique to culture corneal epithelial stem cells on temperature-responsive culture dishes, fabricate multilayered corneal epithelial cell sheets and harvest them, (2) we have purified SP cells from limbal epithelium and enriched corneal epithelial progenitor cells by cell sorting using cell surface markers, (3) we have developed surgical techniques for transplantation of the cell sheets, (4) we have developed an effective method for gene transfer to the corneal epithelial cell sheets. Regarding the project for development of corneal endothelial cell sheet transplantation, (5) we have developed culture methods of human corneal endothelial cells, (6) we have developed a technique to culture corneal endothelial cells on temperature-responsive culture dishes, fabricate corneal endothelial cell sheets and harvest them. These results allow us to use our developed technologies for clinical use.
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[Journal Article] Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces.2006
Author(s)
Sumide T, Nishida K, Yamato M, Ide T, Hayashida Y, Watanabe K, Yang J, Kohno C, Kikuchi A, Maeda N, Watanabe H, Okano T, Tano Y
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Journal Title
FASEB J 20
Pages: 392-394
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface.2004
Author(s)
Nishida K, Yamato M, Hayashida Y, Watanabe K, Maeda N, Watanabe H, Yamamoto K, Nagai S, Kikuchi A, Tano Y, Okano T
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Journal Title
Transplantation 77
Pages: 379-385
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Corneal Reconstruction Using Tissue-Engineered Cell Sheets Comprising Autologous Oral Mucosal Epithelium.2004
Author(s)
Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, Adachi E, Nagai S, Kikuchi A, Maeda N, Watanabe H, Yamamoto K, Okano T, Tano Y
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Journal Title
N Engl J Med 351
Pages: 1187-1196
Description
「研究成果報告書概要(欧文)」より
