2005 Fiscal Year Final Research Report Summary
Basic study on cartilage, bone and tendon tissue engineering by using physical factors
| Project/Area Number |
15390539
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Saitama Medical School |
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Principal Investigator |
NAKATSUKA Takashi Saitama Medical School, Plastic Surgery, Professor, 医学部, 教授 (80198134)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIOKA Shigeru Saitama Medical School, Plastic Surgery, Associate Professor, 医学部, 助教授 (60306272)
TOKIOKA Kazuyuki Saitama Medical School, Plastic Surgery, Lecturer, 医学部, 講師 (70332616)
TAKATO Tsuyoshi The University of Tokyo Hospital, Oral and Maxillofacial Surgery, Professor, 医学部, 教授 (90171454)
NAKAMURA Kozo The University of Tokyo Hospital, Orthopedic Surgery, Professor, 医学部, 教授 (60126133)
HOSHI Kazuto The University of Tokyo Hospital, Contribute lectures staff (associate professor), 医学部, 助教授 (30344451)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Regenerative Medicine / Physical factor / Scaffold / Differentiation / Proliferation |
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| Research Abstract |
A social concern has risen to the maintenance of quality of life (QOL). Aiming at the maintenance of QOL by making tissue engineered bone, cartilage and tendon, we tried to use the characteristics of such tissues [i.e. ; they sensitively react to physical factors such as a mechanical stress, a magnetic stimulation or etc]. First, the cell proliferation and the differentiation condition were optimized. To prepare a mesenchymal cell proliferation medium that does not contain fetal bovine serum (FBS), and that provides more than a 1000-fold increase in cell numbers within approximately a month, we investigated the combinational effects of 12 factors on the proliferation of human cells by analysis of variance in fractional factorial design. As a result, FGF-2, dexamethasone, insulin, and IGF-I possessed promotional effects on proliferation on chondrocytes, while the combination of FGF-2 with insulin or IGF-I synergistically enhanced the proliferation. Thus we concluded that such a combination of FGF-2 with insulin or IGF-I may be useful for promotion of auricular chondrocyte proliferation in a clinical application for cartilage regeneration. Next we discovered that the combination of BMP-2, insulin, and thyroid hormone (BIT) is suitable for resolving the problem of de-differentiation of the chondrocyte caused along with the long time cell culture. Then, we studied the characteristics of some hydrogels expected to be used in clinical application by investigating their effects on cell proliferation or differentiation. (228 words)
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Research Products
(62 results)
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[Journal Article] Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials.2006
Author(s)
Yamaoka, H., Asato, H., Ogasawara, T., Nishizawa, S., Takahashi, T., Nakatsuka, T., Koshima, I., Nakamura, K., Kawaguchi, H., Chung, U-I, Takato, T., Hoshi K.
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Journal Title
J Biomed Mater Res (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Synergistic effects of FGF-2 with insulin or IGF-I on the proliferation of human auricular chondrocytes.2005
Author(s)
Takahashi, T., Ogasawara, T., Kishimoto, J., Liu, G., Asato, H., Nakatsuka, T., Uchinuma, E., Nakamura, K., Kawaguchi, H., Takato, T., Hoshi, K.
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Journal Title
Cell Transplant 14
Pages: 683-693
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibition of Cdk6 expression through p38 MAP kinase is involved in differentiation of mouse prechondrocyte ATDC5.2005
Author(s)
Moro, T., Ogasawara, T., Chikura, H., Ikeda, T., Ogata, N., Maruyama, Z., Komori, T., Hoshi, K., Chung, U.I., Nakamura, K., Okayama, H., Kawaguchi, H.
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Journal Title
J Cell Physiol 204
Pages: 927-933
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Osteoarthritis development in novel experimental mouse models induced by knee joint instability.2005
Author(s)
Kamekura, S., Hoshi, K., Shimoaka, T., Chung, U., Chikuda, H., Yamada, T., Uchida, M., Ogata, N., Seichi, A., Nakamura, K., et al.
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Journal Title
Osteoarthritis Cartilage 13
Pages: 632-641
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner.2005
Author(s)
Yano, F., Kugimiya, F., Ohba, S., Ikeda, T., Chikuda, H., Ogasawara, T., Ogata, N., Takato, T., Nakamura, K., Kawaguchi, H., Chung, U.I.
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Journal Title
Biochem Biophys Res Commun 333
Pages: 1300-1308
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Deficiency of insulin receptor substrate-1 impairs skeletal growth through early closure of epiphyseal cartilage.2004
Author(s)
Hoshi, K., Ogata, N., Shimoaka, T., Terauchi, Y., Kadowaki, T., Kenmotsu, S., Chung, U.I., Ozawa, H., Nakamura, K., Kawaguchi, H.
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Journal Title
J Bone Miner Res 19
Pages: 214-223
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Osteoclast differentiation by RANKL requires NF-kappaB-mediated downregulation of cyclin-dependent kinase 6 (Cdk6).2004
Author(s)
Ogasawara, T., Katagiri, M., Yamamoto, A., Hoshi, K., Takato, T., Nakamura, K., Tanaka, S., Okayama, H., Kawaguchi, H.
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Journal Title
J Bone Miner Res 19
Pages: 1128-1136
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Bone morphogenetic protein 2-induced osteoblast differentiation requires Smad-mediated down-regulation of Cdk6.2004
Author(s)
Ogasawara, T., Kawaguchi, H., Jinno, S., Hoshi, K., Itaka, K., Takato, T., Nakamura, K., Okayama, H.
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Journal Title
Mol Cell Biol 24
Pages: 6560-6568
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes.2004
Author(s)
Chikuda, H., Kugimiya, F., Hoshi, K., Ikeda, T., Ogasawara, T., Shimoaka, T., Kawano, H., Kamekura, S., Tsuchida, A., Yokoi, N., Nakamura, K., Komeda, K., Chung, U.I., Kawaguchi, H.
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Journal Title
Genes Dev 18
Pages: 2418-2429
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The combination of SOX5, SOX6, and SOX9 (the SOX trio) provides signals sufficient for induction of permanent cartilage.2004
Author(s)
Ikeda T., Kamekura S., Mabuchi A., Kou I., Seki S., Takato T., Nakamura K., Kawaguchi H., Ikegawa S., Chung UI.
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Journal Title
Arthritis Rheum 50(11)
Pages: 3561-3573
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Global analysis of shear stress-responsive genes in vascular endothelial cells.2003
Author(s)
Ohura N., Yamamoto K., Ichioka S., Sokabe T., Nakatsuka H., Baba A., Shibata M., Nakatsuka T., Harii K., Wada Y., Kohro T., Kodama T., Ando J.
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Journal Title
J Atheroscler Thromb 10
Pages: 304-313
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cystatin 10, a novel chondrocyte-specific protein, may promote the last steps of the chondrocyte differentiation pathway.2003
Author(s)
Koshizuka, Y., Yamada, T., Hoshi, K., Ogasawara, T., Chung, U.I., Kawano, H., Nakamura, Y., Nakamura, K., Ikegawa, S., Kawaguchi, H.
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Journal Title
J Biol Chem 278
Pages: 48259-48266
Description
「研究成果報告書概要(欧文)」より
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[Book] 新家庭の医学2005
Author(s)
中塚貴志 ほか
Total Pages
1560
Publisher
時事通信社
Description
「研究成果報告書概要(和文)」より
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