2004 Fiscal Year Final Research Report Summary
Vascularization of Regenerated Tissue by Therapeutic Angiogenesis based on the Bio-Regulation System
| Project/Area Number |
15390540
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Saitama Medical School |
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Principal Investigator |
ICHIOKA Shigeru Saitama Medical School, Assistant Professor, 医学部, 助教授 (60306272)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATSUKA Takashi Saitama Medical School, Professor, 医学部, 教授 (80198134)
SHIBATA Masahiro University of Tokyo, Associate Professor, 大学院・医学系研究科, 講師 (60158954)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Angiogenesis / Regenerative Medicine / Bone Marrow |
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| Research Abstract |
This study attempted to clarify the effects of therapeutic neovascularization by bone marrow cells for salvaging flaps after ischemia-reperfusion injury. Bone marrow mononuclear cell layer (endothelial progenitor cell-enriched fraction) was isolated from the mouse femur and tibia. Symmetrical double flaps were elevated in mice. Each flap topically received phosphate buffered saline (PBS) or bone marrow cells in PBS. Flaps were subjected to 6-hour ischemia and subsequent reperfusion. On the 7^<th> postoperative day, the flap survival area was measured (n=27). The mean survival area of bone marrow cells -transplanted flaps was 66.3 ± 18.0 % whereas control flaps showed a survival area of 49.7± 22.2 %. The difference was highly significant (p=0.000209). Histological examination revealed the average vascular density of bone marrow cells-transplanted flaps had significantly increased. The present study proved bone marrow cells acted with significant efficacy in promoting the survival of ischemia-reperfusion-mediated damaged tissue
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Research Products
(6 results)
