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2005 Fiscal Year Final Research Report Summary

Application of the CCN gene family to the regulation of osteogenic stem cells and restitutive medicine

Research Project

Project/Area Number 15390552
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KATSUBE Ken-ichi  Tokyo Medical and Dental University, Graduate School of Medical and Dental Research, assistant professor, 大学院・医歯学総合研究科, 講師 (20233760)

Co-Investigator(Kenkyū-buntansha) SAKAMOTO Kei  Tokyo Medical and Dental University, Graduate School of Medical and Dental Research, assistant, 大学院・医歯学総合研究科, 助手 (00302886)
NAKANISHI Toru  Shujitsu University, Dept.Pharmacology, Professor, 薬学部, 教授 (30243463)
UMEZAWA Akihiro  National Institute of Child Health and Development, Director, 部長 (70213486)
KUDO Akira  Tokyo Institute of Technology, Graduate School of Life Sciences, Professor, 大学院・生命理工学研, 教授 (70178002)
Project Period (FY) 2003 – 2005
Keywordsnotch / CCN / stem cells / restitutive medicine
Research Abstract

There will be more needs for the restitutive medicine of bone and dentine in near future and it is an important initial step to clarify their molecular basis. Particularly, establishment and development of the stem cells that are related to the bone or dentine formation is fundamental, but until now it was not easy to make the cell lines that have high potential of differentiation ability but without tumorigenesis. We focused on the CCN and Notch signal that are important for the stem cell regulation and applied them for a mesenchymal stem cell line that has osteogenic ability.
When the notch signal is constitutively activated in this cell line, the potential of osteogenesis is suppressed while neurogenic potential was upregulated. Global gene expression analysis using gene chip methods revealed that CCN3 (former name nephroblastoma overexpressed gene, Nov) dynamically changed its expression level corresponding to the notch signal. Then we constructed several truncated forms of the CCN3 to know its role in osteogenesis in this cell line. CCN3 was previously reported to express in a specific pattern during bone restitution and to associate with the exracellular domain of Notch protein to stimulate its signal. Several truncated forms greatly upregulated the osteogenesis, which indicates their dominant negative effects. We got several important progress in the osteogenic restitutive medicine indicating the possibility of the novel development of drugs.

  • Research Products

    (24 results)

All 2006 2005 2004 2003 Other

All Journal Article (23 results) Book (1 results)

  • [Journal Article] Stratum intermedium lineage diverges from ameloblast lineage via Notch signaling.2006

    • Author(s)
      Harada H, Ichimori Y, Yokohama-Tamaki T, Ohshima H, Kawano S, Katsube K, Wakisake S.
    • Journal Title

      Biochem Biophys Res Commun. 10;340:6

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stratum intermedium lineage diverges from ameloblast lineage via Notch signaling.2006

    • Author(s)
      Harada H, Ichimori Y, Yokohama-Tamaki T, Ohshima H, Kawano S, Katsube K, Wakisaka S.
    • Journal Title

      Biochem Biophys Res Commun. 340(2)

      Pages: 611-616

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tlle fetsl dernlal but not loose fascial mesenchymal cells possess regellerative activity of dermal structure.2005

    • Author(s)
      Kishi K, Katsube K, Satoh H, Imanishi N, Nakajima H, Nakajima T.
    • Journal Title

      Cell Transplantation 14(9)

      Pages: 709-14

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Notch in vertebrates--molecular aspects of the signal.2005

    • Author(s)
      Katsube K, Sakamoto K.
    • Journal Title

      Int J Dev Biol. 49(2-3)

      Pages: 369-74

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa.2005

    • Author(s)
      Kawashima N, Shindo K, Sakamoto K, Kondo H, Umezawa A, Kasugai S, Perbal B, Suda H, Takagi M, Katsube K.
    • Journal Title

      J Bone Miner Metab. 23(2)

      Pages: 123-33

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Distinct roles of EGF repeats for the Notch signaling system.2005

    • Author(s)
      Sakamoto K, Chao WS, Katsube K, Yamaguchi A.
    • Journal Title

      Exp Cell Res. 15;302

      Pages: 281-91.

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa.2005

    • Author(s)
      Kawashima N, <途中8名>, Katsube K
    • Journal Title

      J. Bone Min. Met. 23(2):

      Pages: 123-33

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The fetal dermal but not loose fascial mesenchymal cells possess regenerative activity of dermal structure. Cell2005

    • Author(s)
      Kishi K, Katsube K, Satoh H, Imanishi N, Nakajima H, Nakajima T.
    • Journal Title

      Transplant. 14(9)

      Pages: 709-714

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Distinct role of EGF repeats for the Notch signaling system.2005

    • Author(s)
      Sakamoto K, Wang SC, Katsube K, Yamaguchi A
    • Journal Title

      Exp.Cell Res. 23(2)

      Pages: 123-133

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells2005

    • Author(s)
      Kawashima N, Katsube K.
    • Journal Title

      J.Bone Min.Met. 23(2)

      Pages: 123-133

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Coexpression of heparanase, basic fibroblast growth factor and vascular endothelial growth factor in human esophageal carcinomas..2004

    • Author(s)
      Mikami S*, Ohashi K, Katsube K, <以下3名>
    • Journal Title

      Pathol Int. 54(8)

      Pages: 556-563.

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transient receptor potential vanilloid subfamily l expressed in pancreatic islet beta cells modulates insulin seretion in rats.2004

    • Author(s)
      Akiba Y, Kato S, Katsube K, <以下4名>
    • Journal Title

      Biochem. Biohpy. Res. Comm 321:

      Pages: 219-225.

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] R-spondin, a Novel gene with thrombospondin type l domain, expressed in the dorsal neural tube and affected in Wnts Mutants.2004

    • Author(s)
      Kamata T, Katsube K, <以下4名>
    • Journal Title

      Biochimica et Biophysica Acta. 1676(1)

      Pages: 51-62.

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Coexpression of heparanase, basic fibroblast growth factor and vascular endothelial growth factor in human esophageal carcinomas.2004

    • Author(s)
      Mikami S^*, Ohashi K, Katsube K, et al.
    • Journal Title

      Pathol Int. 54(8)

      Pages: 556-563

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transient receptor potential vanilloid subfamily 1 expressed in pancreatic islet beta cells modulates insulin secretion in rats.2004

    • Author(s)
      Akiba Y, Kato S, Katsube K, et al.
    • Journal Title

      Biochem.Biophy.Res.Comm. 321

      Pages: 219-225

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] R-spondin, a Novel gene with thrombospondin type 1 domain, expressed in the dorsal neural tube and affected in Wnts mutants2004

    • Author(s)
      Kamata T, Katsube K et al.
    • Journal Title

      Biochimica et Biophysica Acta 1676(1)

      Pages: 51-62

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Osteogenic differentiation of the mesenchymal progenitor cells, Kusa is suppressed by Notch signaling.2003

    • Author(s)
      Shindo K, <途中4名>, Katsube K, Suda H.
    • Journal Title

      Exp. Cell Res 94(3)

      Pages: 370-380

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Heparanase and tumor invasion patterns in human oral squamous cell carcinoma xenografts2003

    • Author(s)
      Kurokawa H, Katsube K,.
    • Journal Title

      Cancer Sci.. 94(3):

      Pages: 277-285.

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Heparanase and tumor invasion patterns in human oral squamous cell carcinoma xenografts2003

    • Author(s)
      Kurokawa H, Katsube K,.
    • Journal Title

      Mol. Pathol 56(2):

      Pages: 127-128.

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Osteogenic differentiation of the mesenchymal progenitor cells, Kusa is suppressed by Notch signaling.2003

    • Author(s)
      Shindo K, Katsube K, Suda H
    • Journal Title

      Exp.Cell Res. 290(2)

      Pages: 370-380

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Heparanase and tumor invasion patterns in human oral squamous cell carcinoma xenografts.2003

    • Author(s)
      Kurokawa H, Katsube K, et al.
    • Journal Title

      Cancer Sci.(formerly, Jpn.J.Cancer Res) 94(3)

      Pages: 277-285

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Proposal for a unified CCN nomenclature.2003

    • Author(s)
      Brigstock DR, Goldschmeding R, Katsube K, et al.
    • Journal Title

      Mol.Pathol. 56(2)

      Pages: 127-128

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human salivary gland mucoepidermoid carcinomas expressed the Hairy/Enhancer of Split homologue 1 (HES1), a downstream factor of Notch signal.

    • Author(s)
      Ohno F, Katsube K
    • Journal Title

      Oral Pathol.Med. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] CCN Proteins2005

    • Author(s)
      Katsube K<共著>
    • Total Pages
      311
    • Publisher
      Imperial College Press
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13   Modified: 2021-12-17  

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