2006 Fiscal Year Final Research Report Summary
Investigation of relationship between Osterix and Cbfal during BMP2-induced osteoblast differentiation
| Project/Area Number |
15390560
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
NISHIMURA Riko Osaka University, Graduate School of Dentistry, Associate Professor, 大学院歯学研究科, 助教授 (60294112)
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| Co-Investigator(Kenkyū-buntansha) |
YONEDA Toshiyuki Osaka University, Graduate School of Dentistry, Professor, 大学院歯学研究科, 教授 (80142313)
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| Project Period (FY) |
2003 – 2006
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| Keywords | osteoblast / adipocyte / Osterix / Cbfa1 / Runx2 |
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| Research Abstract |
1.Role of Osterix in osteoblast differentiation
We found that overexpression of Osterix promoted differentiation of mesenchymal cells into osteoblasts. Microarray analysis indicated that Osterix has common and distinct target genes from Cbfa1. Therefore, our findings suggest that Osterix not only functions as a downstream of Cbfa1 but also regulates osteoblastogenesis independently of Cbfa1.
2.Cbfa1-indepenednt regulation of Osterix expression
We demonstrated that BMP2 induced Osterix expression in Cbfa1 deficient mesenchymal cells. Induction of Osterix in the cells was mediated by Smad signaling and homeobox gene.
3.Target genes of Osterix
To identify the target genes of Osterix, we performed microarray analysis and found that a transcriptional regulatory protein containing RRM motif is involved in osteoblast differentiation.
4.Role of Osterix and Cbfa1 in Ihh-mediated osteoblast differentiation
To understand the molecular basis by which Ihh stimulates osteoblast differentiation, we investigate the role of Osterix and Cbfa1 in this process. We found that Gli2, activated by Ihh, regulated expression and function of Runx2 but not those of Osterix. These results indicate that Gli2 promotes osteoblast differentiation by controlling Cbfa1.
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Research Products
(36 results)
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[Book] 先端歯科医学の創生2005
Author(s)
西村 理行 ら
Total Pages
273(11)
Publisher
大阪大学出版会
Description
「研究成果報告書概要(和文)」より
