• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

Molecular analyses and identifications of new genes to infectious diseases relating with oral biofilm

Research Project

Project/Area Number 15390571
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

SENPUKU Hidenobu  National Institute of Infectious Diseases, Department of Bacteriology, Laboratory Head, 細菌第一部, 室長 (20250186)

Co-Investigator(Kenkyū-buntansha) NAKAO Ryoma  National Institute of Infectious Diseases, Department of Bacteriology, Researcher, 細菌第一部, 研究員 (10370959)
WATANABE Haruo  National Institute of Infectious Diseases, Department of Bacteriology, Director, 細菌第一部, 部長 (70142130)
HANADA Nobuhiro  National Institute of Infectious Diseases, Department of Oral Health, Director, 口腔保健部, 部長 (70180916)
Project Period (FY) 2003 – 2005
Keywordsmutans streptococci / oral biofilm / quorum sensing (QS) system / DNA micro array / Flow cell system / bacitracin transport ATP-binding protein bcrA / RT-PCR / CSP
Research Abstract

A biofilm forming is associated with bacterial pathogenicity and escape from immunological effects in bacterial infectious diseases such as dental caries. Many investigators studied indirectly mechanism of oral biofilm in vitro assay, which is not similar to oral condition. In the present study, we attempted to identify susceptible genes for biofilm formation of Streptococcus mutans in similar condition to oral cavity. Further, we analyzed relationship between the gene expression and host gene (e2f-1) that response to byposalivation in mouse. Comparison of the intensity of biofilm formation by S.mutans clinical strains in flow cell system with the gene expression was firstly investigated. Seventeen different patterns of genomic DNA were identified in S.mutans organisms isolated from biofilm samples taken from 3-year-old children and their mothers by pulsed-field gel electrophoresis. The S.mutans isolates showed differing abilities to form biofilms on flow cell system and the FSC-3 and FSC-4 strains showed the greatest and least, respectively, levels of biofilm formation when examined with confocal laser scanning microscopy (CLSM). Further, in a comparison of FSC-3 and -4 biofilms, DNA microarray results showed 74 genes were differentially expressed more than 4-fold. We identified more than 60% CSP-induced genes of 74 in quorum sensing (QS) system, including bacitracin transport ATP-binding protein bcrA and PTS system IIA, B, C and D. Analyses of the bcrA deficient strains showed significant deceasing of biofilm formation and attachment on the tooth surface in E2F-1^<-/-> mouse in comparison with wild strain. Our results showed that these genes may play biofilm formation and interact with E2F-1 gene in host.

  • Research Products

    (29 results)

All 2006 2005 2004 2003 Other

All Journal Article (24 results) Patent(Industrial Property Rights) (5 results)

  • [Journal Article] Post-operative infection by pathogenic microorganisms in the oral cavity of patients with prostatic carcinoma2006

    • Author(s)
      Senpuku H, Tada A, Uehara S, Kariyama R, Kumon H
    • Journal Title

      Journal International Medical Research 34・1

      Pages: 95-102

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Human T cell responses to oral streptococci in human PBMC-NOD/SCID mice2006

    • Author(s)
      Salam MA, Nakao R, Yonezawa H, Watanabe H, Senpuku H
    • Journal Title

      Oral Microbiology and Immunology 21・3

      Pages: 169-176

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Post-operative infection by pathogenic microorganisms in the oral cavity of patients with prostatic carcinoma2006

    • Author(s)
      Senpuku H, Tada A, Uehara S, Kariyama R, Kumon H
    • Journal Title

      Journal International Medical Research 34

      Pages: 95-102

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human T cell responses to oral streptococci in human PBMC-NOD/SCID mice2006

    • Author(s)
      Salam MA, Nakao R, Yonezawa H, Watanabe H, Senpuku H
    • Journal Title

      Oral Microbiology and Immunology 21

      Pages: 169-176

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Telomerase size and teloinerase activity in Epstein-Barr virus (EBV)-positive and EBV-negative Burkitt's lymphoma cell lines. Archives of Virology.2005

    • Author(s)
      Mochida A, Gotoh E, Senpuku H, Harada S, Kitaimura R, Takahashi T, K. Yanagi K
    • Journal Title

      Archives of Virology 50・10

      Pages: 2139-2150

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Telomerase size and tlomerase activity in Epstein-Barr virus (EBV)-positive and EBV negative Burkitt's lymphoma cell lines. Archives of Virology.2005

    • Author(s)
      Mochida A, Gotoh E, Senpuku H, Harada S, Kitamura R, Takahaslu T, K.Yanagi K
    • Journal Title

      Archives of Virology 50

      Pages: 2139-2150

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Maintenance of serum immunoglobulin G antibodies to Epstein Barr Virus (EBV) nuclear antigen 2 in healthy individuals from different age groups in Japanese population with a high childhood incidence of asymptomatic primary EBV infection.2004

    • Author(s)
      S.Harada, H.Senpuku et al.
    • Journal Title

      Clinical Diagnostic Laboratory Immunology 11

      Pages: 123-130

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment of animal model for initial adhesion of oral streptococci using recombinant NOD.B10.D2 mice.2004

    • Author(s)
      Md A.Salam, N.Matsumoto, K.Matin, H.Senpuku et al.
    • Journal Title

      Clinical Diagnostic Laboratory Immunology 11

      Pages: 379-386

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role of gene E2f1 in susceptibility to bacterial adherence of oral streptococci to tooth surfaces in mice.2004

    • Author(s)
      N.Matsumoto, Md A.Salam, T.Amagasa, H.Senpuku
    • Journal Title

      Oral Microbiology and Immunology 19

      Pages: 270-276

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Relationship between volatile sulfur compounds concentration and oral bacteria species detection in the elderly.2004

    • Author(s)
      H.Senpuku, A.Tada, T.Yamaga, N.Hanada, H.Miyazaki
    • Journal Title

      International Dental Journal 54

      Pages: 149-153

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Systemic diseases in association with microbial species in oral biofilm from elderly requiring care.2004

    • Author(s)
      Y.Tsuha, N.Hanada, T.Asano, T.Aei, H.Senpuku, et al.
    • Journal Title

      Clinical Exp.erimental Immunology 137

      Pages: 393-401

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular interaction of surface protein peptides of Streptococcus gordonii with human salivary components.2004

    • Author(s)
      T.Hamada, M.Kawashima, J.Tagaini, H.Senpuku
    • Journal Title

      Infection and Immunity 72

      Pages: 4819-4826

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role of gene E2f1 in susceptibility to bacterial adherence of oral streptococci to tooth surfaces in mice.2004

    • Author(s)
      N.Matsumoto, Md., A.Salam, T.Amagasa, H.Senpuku
    • Journal Title

      Oral Microbiology and Immunology 19

      Pages: 270-276

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular interaction of surface protein peptides of Streptococcus gordonii with human salivary components.2004

    • Author(s)
      T.Hamada, M.Kawashima, J.Tagami, H.Senpuku
    • Journal Title

      Infection and Immunity 72

      Pages: 4819-4826

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bone regeneration by recombinant human bone morphogenetic protein-2 (rhBMP-2) around immediate implants : A pilot study in Rats.2003

    • Author(s)
      K.Matin, H.Senpuku, N.Hanada, H.Ozawa, S.Ejiri.
    • Journal Title

      International Journal Oral Maxillofacical Implants 18

      Pages: 211-217

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Assessment of oral transmission using cell-free HIV-1 in mice reconstituted with human peripheral blood leukocyte.2003

    • Author(s)
      R.Nakao, H.Senpuku et al.
    • Journal Title

      Immunology 109

      Pages: 271-282

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Real-time interaction of oral streptococci with human salivary components..2003

    • Author(s)
      M.Kawashima, N.Hanada, T.Hamada, J.Tagami, H.Senpuku
    • Journal Title

      Oral Microbiology and Immunology 18

      Pages: 220-225

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Systemic diseases in association with microbial species in oral biofilm from elderly requiring care.2003

    • Author(s)
      H.Senpuku, A.Sogame, E.Inoshita, Y.Tsuha, et al.
    • Journal Title

      Gerontology 49

      Pages: 301-309

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Bone regeneration by recombinant human bone morphogenetic protein-2 (rhBMP-2) around inunediate implants: A pilot study in Rats.2003

    • Author(s)
      K.Matin, H.Senpuku, N.Hanada, H.Ozawa, S.Ejiri.
    • Journal Title

      International Journal Oral Maxillofacical Implants 18

      Pages: 211-217

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Assessment of oral transmission using cell-free HIV -1 in mice reconstituted with human peripheral blood leukocyte.2003

    • Author(s)
      R.Nakao, H.Senpuku et al.
    • Journal Title

      Immunology 109

      Pages: 271-282

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Real-time interaction of oral streptococci with human salivary components.2003

    • Author(s)
      M.Kawasima, N.Hanada, T.Hamada, J.Tagami, H.Senpuku
    • Journal Title

      Oral Microbiology and Immunology 49

      Pages: 301-309

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Maintenance of serum immunoglobulin G antibodies to Epstein Barr Vines (EBV) nuclear antigen 2 in healthy individuals from different age groups in Japanese population with a high childhood incidence of asymptomatic primary EBV infection.2003

    • Author(s)
      S.Harada, H.Senpuku et al.
    • Journal Title

      Clinical Diagnostic Laboratory Immunology 11

      Pages: 123-130

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Association between commensal bacteria and opportunistic pathogens in the dental plaque of elderly individuals

    • Author(s)
      Tada A, Senpuku H, Motozawa Y, Yoshihara A, Hanada N, Tanzawa H
    • Journal Title

      Clinical Microbiology and Infection (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Association between commensal bacteria and opportunistic pathogens in the dental plaque of elderly individuals

    • Author(s)
      Tada A, Senpuku H, Motozawa Y, Yoshihara A, Hanada N, Tanzawa H
    • Journal Title

      Clinical Microbiology and Infection (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] う蝕リスク検査方法2004

    • Inventor(s)
      泉福英信, 鱒沢諭美子
    • Industrial Property Rights Holder
      泉福英信, 鱒沢諭美子
    • Industrial Property Number
      アメリカ 出願番号11/013-430
    • Filing Date
      2004-12-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] う蝕リスク検査方法2003

    • Inventor(s)
      泉福英信, 鱒沢諭美子
    • Industrial Property Rights Holder
      泉福英信, 鱒沢諭美子
    • Industrial Property Number
      EPC 出願番号03026551.6
    • Filing Date
      2003-11-18
    • Acquisition Date
      2006-03-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] う蝕リスク検査方法2003

    • Inventor(s)
      泉福英信, 鱒沢諭美子
    • Industrial Property Rights Holder
      泉福英信, 鱒沢諭美子
    • Industrial Property Number
      アメリカ 出願番号10/714-852
    • Filing Date
      2003-11-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] う蝕リスク検査方法2003

    • Inventor(s)
      泉福英信, 鱒沢諭美子
    • Industrial Property Rights Holder
      泉福英信, 鱒沢諭美子
    • Industrial Property Number
      中国 出願番号200310119573.8
    • Filing Date
      2003-10-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] う蝕リスク検査方法2003

    • Inventor(s)
      泉福英信, 鱒沢諭美子
    • Industrial Property Rights Holder
      泉福英信, 鱒沢諭美子
    • Industrial Property Number
      日本 出願番号2003-433412
    • Filing Date
      2003-12-16
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi