2004 Fiscal Year Final Research Report Summary
Identification of intracellular factors involved with apoptosis induction by TGF-β family
| Project/Area Number |
15390621
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Kyushu Dental College |
|---|
Principal Investigator |
FUKUDA Jinichi Kyushu Dental College, Faculty of Dentistry, Professor, 歯学部, 教授 (00106270)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Kazuhuro Kyushu Dental College, Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (40188793)
YOSHIOKA Izumi Kyushu Dental College, Faculty of Dentistry, Research Associate, 歯学部, 助手 (10305823)
NISHIHARA Tatsuji Kyushu Dental College, Faculty of Dentistry, Professor, 歯学部, 教授 (80192251)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Keywords | Activin / BMP-2 / GDF-5 / TGF-β family / cell cycle arrest / apoptosis / retinoic acid / malignant transformation |
|---|
| Research Abstract |
Members of TGF-β superfamily are implicated in regulation of various life phenomenons, including initial development, organ formations and tumor formation. We found that activin A and BMP-2 inhibited cell growth of B cell hybridoma, HS-72 and examined its intracellular transduction mechanism. In the present study, we used three kinds of cells (HS-72 cells, LS-1 cells, and LS-12 cells) and investigated difference of sensitivity for activin A to these cells by flow cytometric analysis.
We reported that activin A induced cytotoxic effects to HS-72, and induced G1 arrest in cell cycle and apoptosis at the concentration of 5-50 ng/ml. We clearly that retinoic acid induced a littel cytotoxity to HS-72 and G1 arrest in cell cycle, and that co-stimulation of retinoic acid and activin A potently induced G1 arrest and apoptosis. Activin A or retinoic acid have no effect to LS-1, however, co-stiluration of these factors induced G1 arrest and inclease of sub G1 rate of LS-1 cells. LS-12 showed intermediate type to Activin A and/or retinoic acid.
Taken together, the results obtained in this study suggest that cross-talks between activin A and retinoic acid play an important role of the inductin of apoptosis in B lineage cells.
|
