2005 Fiscal Year Final Research Report Summary
Investigation of Unutilized Bioluminescent Systems and Fluorescent Proteins, and Development of New Molecular Proves.
| Project/Area Number |
15404009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Electro-Communications |
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Principal Investigator |
NIWA Haruki The University of Electro-Communications, Faculty of Electro-communications, Professor, 電気通信学部, 教授 (20135297)
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Takashi The University of Electro-Communications, Faculty of Electro-communications, Associate Professor, 電気通信学部, 助教授 (20238380)
MAKI Shojiro The University of Electro-Communications, Faculty of Electro-communications, Assistant Professor, 電気通信学部, 助手 (20266349)
OHMIYA Yoshihiro National Institute of Advanced Industrial Science and Technology, Research Institute for Cell Engineering, Group Reader, 人間系特別研究体, グループ長 (20223951)
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| Project Period (FY) |
2003 – 2005
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| Keywords | bioluminescence / luminous worm / Odotosyllis / luminous snail / Latia / liciferin / liciferase |
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| Research Abstract |
1)From the marine luminescent worm, Odontosyll is phosphorea collected off from Mission Bay, San Diego, U.S.A., the light producing substance, Odontosyll is luciferin was isolated under the guidance of luciferin-luciferase reaction. The bioluminescence spectrum (λmax 500nm) using the isolated luciferin and the crude luciferase was similar to that of live specimens. The UV spectrum was essentially identical with that reported previously by Shimomura. The molecular formula of the luciferin was determined from the high resolution ESI mass spectrum to be C_<15>H_8O_<12>S_3.
2)Exploration of luminescent marine organisms was performed at New Caledonia, some luminous worm and sea hare was found.
3)From the fresh-water limpet like snail, Latia neritoides collected at the Nugtunu stream near Mt. Pirongia, New Zealand, the luciferase was isolated under the guidance of luciferin-luciferase reaction. The detailed biochemical studies indicated that the active form of the luciferase was found to be a hexamer of N-linked glycoprotein monomer having the molecular mass 31kDa. The cloning of the luciferase gene was succeeded. However heterologous expression of the gene was not succeeded so far.
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Research Products
(34 results)
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[Journal Article] Bioluminescence Activity of Latia Luciferin Analogues : Replacement of the 2,6,6-Trimethylcyclohexene Ring into the Methyl-substituted Phenyl Groups.2005
Author(s)
Mitsuhiro Nakamura, Masashi Mamino, Mizuki Masaki, Shojiro Maki, Ryo Matsui, Satoshi Kojima, Takashi Hirano, Yoshihiro Ohmiya, Haruki Niwa.
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Journal Title
Tetrahedron Lett. 46, No.1
Pages: 53-56
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Development of the Chemistry of the Imidazopyrazinone-bioluminescence System : From the Bio-and Chemiluminescence Mechanism to a Design of Sensor Molecules.2005
Author(s)
Takashi Hirano, Takashi Sekiguchi, Shunichiro Nakai, Shunsuke Fujio, Shojiro Maki, Haruki Niwa.
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Journal Title
Bioluminescence and Chemiluminescence(ed by A.Tsuji, M.Matsumoto, M.Maeda, L.J.Kricka, P.E.Stanley)(World Scientific), Singapore
Pages: 117-120
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 6,8-Diarylimidazo[11,2a]pyrazin-3(7H)-ones as Potential Chemiluminescent pH/Superoxide Double Sensor.2005
Author(s)
R.Saito, N.Suga, A.Katoh, T.Hirano, H.Niwa.
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Journal Title
Bioluminescence and Chemiluminescence(ed by A.Tsuji, M.Matsumoto, M.Maeda, L.J.Kricka, P.E.Stanley)(World Scientific), Singapore
Pages: 335-338
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Regioselective phenyl-substitution effects on the solvatochromism of 2-phenylimidazo[1,2-a]pyrazin-3(7H)-one derivatives : Expansion of the color variation range of a visible-indicator for the proton donor ability of solvents.2004
Author(s)
Shunsuke Fujio, Daisuke Hashizume, Yoshiharu Takamuki, Masanori Yasui, Fujiko Iwasaki, Shojiro Maki, Haruki Niwa, Hiroshi Ikeda, Takashi Hirano.
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Journal Title
Tetrahedron Lett. 45, No.46
Pages: 8531-8534
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fundamental Studies on The Structure and Spectroscopic Properties of 3,7-Dihydroimidazo[1,2-a]pyrazin-3-one Derivatives.2003
Author(s)
ShunichiroNakai, Masanori Yasui, Masaki Nakazato, Fujiko Iwasaki, Shojiro Maki, Haruki Niwa, Mamoru Ohashi, Takashi Hirano.
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Journal Title
Bull.Chem.Soc.Jpn. 76, No.12
Pages: 2361-2387
Description
「研究成果報告書概要(欧文)」より
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