Molecular epidemiology for protozoan diseases in domestic animals in Xin

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 15405040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: Nihon University
• Principal Investigator: MARUYAMA Soichi Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (30181829)
• Co-Investigator(Kenkyū-buntansha): NOGAMI Sadao Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (90172767) ; KABEYA Hidenori Nihon University, College of Bioresource Sciences, Lecturer, 生物資源科学部, 講師 (10318389) ; XUENAN Xuan Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (10292096) ; INOKUMA Hisashi Obihiro University of Agriculture and Veterinary Medicine, Faculty of Veterinary Medicine, Professor, 畜産学部, 教授 (70263803) ; TSUCHIYA Kotaro Nippon Institute for Biological Science, Chief Scientist, Vice manager, 主任研究員 (70207405)
• Project Period (FY): 2003 – 2006
• Keywords: Xinjiang Uighur Autonomous Region of China / Domestic animals / Molecular epidemiology / Seroepidemiology / Rickettsia / Bacteria / Protozoa / Diagnosis

Research Abstract

We investigated the prevalence of rickettsial and protozoan diseases by serological and molecular techniques in domestic animals in Xinjiang Uygur Autonomous Region, China. Tick DNA samples from cattle were examined for Rickettsia infection by citrate synthase gene-based PCR and sequencing. Four positive samples were detected from Haemaphysalis danieli and high levels of similarity were found with recently detected 'Candidatus Rickettsia principis.' Anaplasma and Ehrlichia infection in domestic animals in Xinjiang area were examined by using an indirect immunofluorescence assay. The antibodies for Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia. The prevalence of Babesia equi and B. caballi infections in donkeys in western Xinjiang was investigated. In total, 93 serum samples were randomly taken from donkeys in the Kashgar and Ili areas, and examined for B. equi and B. caballi infections by ELISA using recombinant antigens. Of the 93 samples, 9 (9.6%) and 36 (38.7%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 2 (2.2%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine babesiosis might be extensively prevalent in donkeys in western Xinjiang.
We explored and evaluated several candidates of specific antigens for protozoan diseases in domestic animals such as Neospora caninum, B. caballi, B. equi, B. gibsoni and B. bovis infections. We also developed several serological diagnostic methods such as ELISA and Immunochromatographic test with those recombinant antigens.

Research Products

• [Journal Article] Detection of rickettsial DNA from ticks recovered from domestic cattle in Xinjiang Uygur autonomous region area, China. (2007)
  • Author(s): Chahan B., Jian Z., Jilintai, Tanabe, S., Miyahara, K., Xuan, X., Sato, Y., Montomo, T., Nogami, S., Mikami, T., Maruyama, S., Inokuma, H.
  • Journal Title: Vet. Parasitol. 144 Pages: 184-187
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular characterization of Rhipicephalus (Boophilus) microplus Bm86 homologue from Haemaphysalis longicornis ticks. (2007)
  • Author(s): Liao, M., Zhou, J., Hatta, T., Umemiya, R., Miyoshi, T., Tsuji, N., Xuan, X., Fujisaki, K.
  • Journal Title: Vet. Parasitol. 146 Pages: 148-157
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Complete nucleotide sequences of the mitochondrial genomes of two avian malaria protozoa, Plasmodium gallinaceum and Plasmodium juxtanucleare. (2007)
  • Author(s): Omori S, Sato Y, Isobe T, Yukawa M, Murata K
  • Journal Title: Parasitol. Res. 100 Pages: 661-664
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Babesia gibsoni ribosomal phosphoprotein PO induces cross-protective immunity against B. microti infection in mice. (2007)
  • Author(s): Terkawi, M.A., Jia, H., Zhou J., Lee, E.G., Igarashi, I., Fujisaki, K., Nishikawa, Y., Xuan, X.
  • Journal Title: Vaccine 25 Pages: 2027-2035
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs. (2007)
  • Author(s): Fukumoto, S., Tamaki, Y., Okamura, M., Bannai, H., Yokoyama, N., Suzuki, T., Igarashi, I., Suzuki, H., Xuan, X.
  • Journal Title: Vaccine 25 Pages: 1334-1341
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Detection of DNA closely related to 'Candidatus Rickettsia principis' in Haemaphysalis danieli recovered from cattle in Xinjiang Uygur Autonomous Region Area, China. (2007)
  • Author(s): Chahan, B., Jian, Z., Jilintai, Miyahara, K., Tanabe, S., Xuan, X., Sato, Y., Moritomo, T., Nogami, S., Mikami, T., Maruyama, S., Inokuma, H.
  • Journal Title: Vet. Parasitol. 144 Pages: 184-187
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] characterization of Rhipicephalus (Boophilus) microplus Bm86 homologue from Haemaphysalis longicornis ticks. (2007)
  • Author(s): Liao, M., Zhou, J., Hatta, T, Umemiya, R., Miyoshi, T, Tsuji, N., Xuan, X., Fujisaki, K.Molecular
  • Journal Title: Vet. Parasitol. 146 Pages: 148-157
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Complete nucleotide sequences of the mitochondrial genomes of two avian malaria protozoa, Plasmodium gallinaceum and Plasmodium juxtanucleare. (2007)
  • Author(s): OmoriS, Sato Y, Isobe T, Yukawa M, Murata K.
  • Journal Title: Parasitol. Res. 100 Pages: 661-664
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Babesia gibsoni ribosomal phosphoprotein PO induces cross-protective immunity against B. microti infection in mice. (2007)
  • Author(s): Terkawi, M.A., Jia, H., Zhou, J., Lee, E.G, Igarashi, I., Fujisaki, K., Nishikawa, Y., Xuan, X.
  • Journal Title: Vaccine 25 Pages: 2027-2035
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs. (2007)
  • Author(s): Fukumoto, S., Tamaki, Y., Okamura, M., Bannai, H., Yokoyama, N., Suzuki, T, Igarashi, I., Suzuki, H., Xuan, X.
  • Journal Title: Vaccine 25 Pages: 1334-1341
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects. (2006)
  • Author(s): Harnnoi, T., Watchabunsook, S., Sakaguchi, T., Xuan, X., Fujisaki, K.
  • Journal Title: J. Vet. Med. Sci. 68 Pages: 1289-1295
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 2006. Immunochromatographic test for simultaneous serodiagnosis of B. caballi and Babesia equi infections in horses. (2006)
  • Author(s): Huang, X., Xuan, X., Verdida, R., Zhang, S., Yokoyama, N., Xu, L., Igarashi, I.
  • Journal Title: Clin. Vaccine Immunol. 13 Pages: 553-555
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evaluation enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infection. (2006)
  • Author(s): Huang, X., Xuan, X., Yokoyama, N., Katayama, Y., Anzai, T., Igarashi, I.
  • Journal Title: Vet. Parasitol. 140 Pages: 158-161
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Seroepidemiological evidence for the possible presence of Babesia (Theileria) equi and Babesia caballi infections in donkeys in Western Xinjiang, China. (2006)
  • Author(s): Chahan, B., Zhang, S., Seo, J., Nakamura, C., Zhang, G., Bannai, H., Jian, Z., Inokuma, H., Tuchiya, K., Sato Y., Kabeya, H., Maruyama, S., Mikami, T., Xuan, X.
  • Journal Title: J. Vet. Med. Sci. 68 Pages: 753-755
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay. (2006)
  • Author(s): Boonchit, S., Alhassan, A., Chan, B., Xuan, X., Yokoyama, N., Ooshiro, M., Goff, W.L., Waghela, S.D., Wagner, G., Igarashi, I.
  • Journal Title: Vet. Parasitol. 137 Pages: 28-35
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Multi-spacer typing to study the genotypic distribution of Bartonella henselae populations. (2006)
  • Author(s): Li, W., Chomel, B., Maruyama, S., Guptil, L., Sander, A., Raoult, D., Fournier, P-E.
  • Journal Title: J. Clin. Microbiol. 44 Pages: 2499-2506
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50. (2006)
  • Author(s): Miyama, T., Inokuma, H., Itamoto, K., Okuda, M., Verdida, R.A., Xuan, X.
  • Journal Title: J. Vet. Med. Sci. 68 Pages: 1371-1373
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Experimental inoculation of beagle dogs with Ehrlichia species detected from Ixodes ovatus. (2006)
  • Author(s): Watanabe, M., Oikawa, T., Hiraoka, H., Kaneko, N., Itamoto, K., Mizuno, H., Okuda, M., Inokuma, H.
  • Journal Title: Vet. Parasitol. 136 Pages: 147-154
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects. (2006)
  • Author(s): Harnnoi, T, Watchabunsook, S., Sakaguchi, T., Xuan, X., Fujisaki, K.
  • Journal Title: J. Vet. Med. Sci. 68 Pages: 1289-1295
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunochromatographic test for simultaneous serodiagnosis of B. caballi and Babesia equi infections in horses (2006)
  • Author(s): Huang, X., Xuan, X., Verdida, R., Zhang, S., Yokoyama, N., Xu, L., Igarashi, I.
  • Journal Title: Clin. Vaccine Immunol 13 Pages: 553-555
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infection. (2006)
  • Author(s): Huang, X., Xuan, X., Yokoyama, N., Katayama, Anzai, T., Igarashi, I.
  • Journal Title: Vet. Parasitol. 140 Pages: 158-161
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Seroepidemiological evidence for the possible presence of Babesia (Theileria) equi and Babesia caballi infections in donkeys in Western Xinjiang, China. (2006)
  • Author(s): Chahan, B., Zhang, S., Seo, J., Nakamura, C., Zhang, G, Bannai, H., Jian, Z., Inokuma, H., Tuchiya, K., Sato, Y, Kabeya, H., Maruyama, S., Mikami, T., Xuan, X.
  • Journal Title: J. Vet. Med. Sci. 68 Pages: 753-755
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1and their potential use in enzyme-linked immunosorbent assay. (2006)
  • Author(s): Boonchit, S., Alhassan, A., Chan, B., Xuan, X., Yokoyama, N., Ooshiro, M., Goff, W.L., Waghela, S.D., Wagner, G, Igarashi, I.
  • Journal Title: Vet. Parasitol. 137 Pages: 28-35
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 (2006)
  • Author(s): Miyama, T, Inokuma, H., Itamoto, K., Okuda, M.Verdida, R.A., Xuan, X.
  • Journal Title: J. Vet. Med. Sci. 68 Pages: 1371-1373
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia. (2005)
  • Author(s): Boldbaatar, D., Xuan, X., Battsetseg, B., Igarashi, I., Battur, B., Batsukh, Z., Bayambaa, B., Fujisaki, K
  • Journal Title: Vet. Parasitol. 127 Pages: 35-38
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis. (2005)
  • Author(s): Hirata, H., Yokoyama, N., Xuan, X., Fujisaki, K., Suzuki, N., Igarashi, I.
  • Journal Title: Clin. Diagn. Lab. Immunol. 12 Pages: 334-338
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Influence of bacteriocin-like substance, generation times, and genetic profiles of Listeria innocua on the isolation of Listeria monocytogenes. (2005)
  • Author(s): Yokoyama, Y, Shibusawa, Y, Maruyama, S, Katsube, Y, Mikami, T
  • Journal Title: Comp. Immun. Microbiol. Infect. Dis. 28 Pages: 177-186
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The marked increase of Listena monocytogenes isolation from contents of swine cecum. (2005)
  • Author(s): Yokoyama, E., Saitoh, T., Maruyama, S., Katsube, Y.
  • Journal Title: Comp. Immunol. Microbiol. Infect. Dis. 28 Pages: 259-268
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Serological evidence of infection of Anaplasma and Ehrlichia in domestic animals in Xinjiang Uygur autonomous region area, China. (2005)
  • Author(s): Chahan B., Jian Z., Xuan, X., Sato, Y., Kabeya, H., Tuchiya, K., Itamoto, K., Okuda, M., Mikami, T., Maruyama, S., Inokuma, H.
  • Journal Title: Vet. Parasitol. 134 Pages: 273-278
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Isolation and functional analysis of chk2 homologue from Entamoeba histolytica. (2005)
  • Author(s): Iwashita, J., Sato, Y., Kobayashi, S., Takeuchi, T., Abe, T.
  • Journal Title: Parasitol. Int. 54 Pages: 21-27
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia. (2005)
  • Author(s): Boldbaatar, D., Xuan, X., Battsetseg, B., Igarashi, I., Battur, B., Batsukh, Z., Bayambaa, B., Fujisaki, K.
  • Journal Title: Vet. Parasitol. 127 Pages: 35-38
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Influence of bacteriocin-like substance, generation times, and genetic profiles of Listeria innocua on the isolation of Listeria monocytogenes. (2005)
  • Author(s): Yokoyama, Y, Shibusawa, Y, Maruyama, S., Katsube, Y, Mikami, T.
  • Journal Title: Comp. Immun. Microbiol. Infect. Dis. 28 Pages: 177-186
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The marked increase of Listeria monocytogenes isolation from contents of swine cecum. (2005)
  • Author(s): Yokoyama, E., Saitoh, T., Maruyama, S., Katsube, Y.
  • Journal Title: Comp. Immunol. Microbiol. Infect. Dis. 28 Pages: 259-268
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serological evidence of infection of Anaplasma and Ehrlichia in domestic animals in Xinjiang Uygur Autonomous Region area, China. (2005)
  • Author(s): Chahan, B., Jian, Z., Xuan, X., Sato, Y, Kabeya, H., Tuchiya, K., Itamoto, K., Okuda.M., Mikami, T., Maruyama, S., Inokuma, H.
  • Journal Title: Vet. Parasitol. 134 Pages: 273-278
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation and functional analysis of chk2 homologue from Entamoeba histolytica. (2005)
  • Author(s): Iwashita, J., Sato, Y, Kobayashi, S., Takeuchi, T., Abe, T.
  • Journal Title: Parasitol. Int 54 Pages: 21-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development and evaluation of an immunochromatographic test for the rapid detection of Babesia equi infection in horses. (2004)
  • Author(s): Huang, X., Xuan, X., Xu, L., Zhang, S., Yokoyama, N., Suzuki, N., Igarashi, I.
  • Journal Title: J. Clin. Microbiol 42 Pages: 359-361
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for the detection of specific antibodies. (2004)
  • Author(s): Boonchit, S., Xuan, X., Yokoyama, N., Goff, W.L., Waghela, S.D., Wagner, G., Igarashi, I.
  • Journal Title: J. Clin. Microbiol 42 Pages: 1601-1604
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. (2004)
  • Author(s): Tamaki, Y., Hirata, H., Takabatake, N., Bork, S., Yokoyama, N., Xuan, X., Fujisaki, K., Igarashi, I.
  • Journal Title: Clin. Diagn. Lab. Immunol. 11 Pages: 211-215
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Serodiagnosis of Babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated P50. (2004)
  • Author(s): Verdida, R.A., Hara, O.A., Xuan, X., Fukumoto, S., Igarashi, I., Zhang, S., Dong, J., Inokuma, H., Kabeya, H., Sato, Y., Moritomom T., Maruyama, S., Claveria F., Nagasawa, H.
  • Journal Title: J. Vet. Med. Sci. 66 Pages: 1517-1521
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genetic diversity of the dnaJ gene in the Mycobacterium avium complex. (2004)
  • Author(s): Morita, Y., Maruyama, S., Kabeya, H., Nagai, A., Kozawa, K., Kato, M., Nakajima, T., Mikami, T., Katsube, Y., Kimura, H.
  • Journal Title: J. Med. Microbiol. 53 Pages: 813-817
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development and evaluation of an immunochromatographic test for the rapid detection of Babesia equi infection in horses. (2004)
  • Author(s): Huang, X., Xuan, X., Xu, L., Zhang, S., Yokoyama, N., Suzuki, N., Igarashi, I.
  • Journal Title: J. Clin. Microbiol. 42 Pages: 359-361
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for specific antibodies. (2004)
  • Author(s): Boonchit, S., Xuan, X., Yokoyama, N., Goff, W.L., Waghela, S.D., Wagner, G, Igarashi, I.
  • Journal Title: J. Clin. Microbiol. 42 Pages: 1601-1604
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis. (2003)
  • Author(s): Hirata, H., Xuan, X., Yokoyama, N., Nishikawa, Y., Fujisaki, K., Suzuki, N., Igarashi, I.
  • Journal Title: J. Clin. Microbiol 41 Pages: 547-551
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High-level expression and purification of a truncated merozote antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis. (2003)
  • Author(s): Huang, X., Xuan, X., Yokoyama, N., Xu, L., Suzuki, H., Sugimoto, C., Naga sawa, H, Fujisaki, K., Igarashi, I
  • Journal Title: J. Clin. Microbiol. 41 Pages: 1147-1151
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAGl. (2003)
  • Author(s): Chahan, B., Gaturaga, I., Huang, X., Min, L., Fukumoto, S., Hirata, H., Nishikawa, Y., Suzuki, H., Sugimoto, C., Igarashi, I., Mikami, T., Xuan, X.
  • Journal Title: Vet. Parasitol. 118 Pages: 177-185
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin province of China. (2003)
  • Author(s): Xu, Y., Zhang, S., Huang, X., Bayin, C., Xuan, X., Igarashi, I., Fujisaki, K., Kabeya, H., Maruyama, S., Mikami, T.
  • Journal Title: J. Vet. Med. Sci. 65 Pages: 1015-1017
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses. (2003)
  • Author(s): Huang, X., Xuan, X., Xu, L., Zhang, S., Yokoyama, N., Suzuki, N., Igarashi, I.
  • Journal Title: J. Clin. Microbiol. 42 Pages: 359-361
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats. (2003)
  • Author(s): Huang, X., Xuan, X., Hirata, H., Yokoyama, N., Xu, L., Suzuki, N., Igarashi, I.
  • Journal Title: J. Clin. Microbiol. 42 Pages: 351-353
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular characterization of a gene encoding 29 kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality. (2003)
  • Author(s): Fukumoto, S., Xuan, X., Inoue, N., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H
  • Journal Title: Mol. Biochem. Parasitol. 131 Pages: 129-136
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High-level expression of truncated major surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity. (2003)
  • Author(s): Fukumoto, S., Xuan, X., Kadota, K.Igarashi I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki.H.
  • Journal Title: Clin. Diagn. Lab. Immunol. 10 Pages: 596-601
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] One-step polymerase chain reaction-based typing of Arcobacter species. (2003)
  • Author(s): Kabeya, H., Kobayashi, Y., Maruyama, S., Mikami, T.
  • Journal Title: Int. J. Food Microbiol. 81 Pages: 163-168
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis. (2003)
  • Author(s): Hirata, H., Xuan, X., Yokoyama, N., Nishikawa, Y., Fujisaki, K., Suzuki, N., Igarashi, I.
  • Journal Title: J. Cin. Microbiol. 41 Pages: 547-551
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-level expression and purification of a truncated merozote antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis. (2003)
  • Author(s): Huang, X., Xuan, X., Yokoyama, N., Xu, L., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Igarashi, I.
  • Journal Title: J. Clin. Microbiol. 41 Pages: 1147-1151
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1. (2003)
  • Author(s): Chahan, B., Gaturaga, I., Huang, X., Min, L., Fukumoto, S., Hirata, H., Nishikawa, Y, Suzuki, H., Sugimoto, C., Igarashi, I., Mikami, T., Xuan, X.
  • Journal Title: Vet. Parasitol. 118 Pages: 177-185
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin province of China. (2003)
  • Author(s): Xu, Y, Zhang, S., Huang, X., Bayin, C., Xuan, X., Igarashi, I., Fujisaki, K., Kabeya, H., Maruyama, S., Mikami, T.
  • Journal Title: J. Vet. Med. Sci. 65 Pages: 1015-1017
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular characterization of a gene encoding 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality. (2003)
  • Author(s): Fukumoto, S., Xuan, X., Inoue, N., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.
  • Journal Title: Mol. Biochem. Parasitol 131 Pages: 129-136
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity. (2003)
  • Author(s): Fukumoto, S., Xuan, X., Kadota, K., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.
  • Journal Title: Clin. Diagn. Lab. Immunol. 10 Pages: 596-601
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] One-step polymerase chain reaction-based typing of Arcobacter species. (2003)
  • Author(s): Kabeya, H., Kobayashi, Y, Maruyama, S., Mikami, T.
  • Journal Title: Int. J. Food Microbiol. 81 Pages: 163-168
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 吸血昆虫と新興感染症 (2007)
  • Author(s): 猪熊 壽(分担執筆)
  • Total Pages: 296
  • Publisher: (株)全国農村教育協会(東京)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 吸血昆虫と新興感染症 (2007)
  • Author(s): 丸山総一(分担執筆)
  • Total Pages: 296
  • Publisher: (株)全国農村教育協会(東京)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 図説獣医寄生虫学 (2006)
  • Author(s): 内田明彦, 野上貞雄, 黄 鴻堅
  • Total Pages: 207
  • Publisher: メディカグローブ
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 動物の感染症 <第二版> (2006)
  • Author(s): 丸山総一(分担執筆)
  • Total Pages: 342
  • Publisher: 近代出版(東京)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 化学療法の領域 (2006)
  • Author(s): 丸山総一(分担執筆)
  • Total Pages: 174
  • Publisher: 医薬ジャーナル社(東京)
  • Description: 「研究成果報告書概要(和文)」より