2004 Fiscal Year Final Research Report Summary
Preparation of microorganism library derived from soils of seacoast, river and lakes, and its utilization for the environmental clean-up from endocrine disrupting chemicals.
| Project/Area Number |
15406006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Nagasaki University |
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Principal Investigator |
UEDA Hiroshi Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00145674)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Makoto Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (60380987)
NAGASAKI Fujita Nagasaki University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (70380855)
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| Project Period (FY) |
2003 – 2004
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| Keywords | endocrine disrupting chemicals / microorganism / contamination / clean-up of waters / source of carbon / microorganism library / Tashiro's carbonless medium |
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| Research Abstract |
The purpose of this research is to discover the soil microorganism to clean-up waters from endocrine disrupting chemicals. In two years research term, we collected soil containing microorganism from several areas where had been polluted by endocrine disrupters. And we made several libraries of soil microorganism possessing ability to remove endocrine disrupting chemicals.
1.Collection of soils from several area where had been polluted by endocrine disrupting chemicals.
We collected soils from following four areas, "the river Aire" in United Kingdom where had been polluted by Nonylphenol, "lake Apopka" in United State by DDT and DDE, "the other side of coast Santa Barbara island" in United State by unknown endocrine disruptors, and "coast Singapore" in Singapore by tributyltin. We also took photos and movies in each place.
2.Making microorganism library.
Bacteria in collected soils were cultured in Tashiro's carbonless medium containing endocrine disrupting chemicals to get survived bacteria which can use these chemicals as the source of carbon. This screening culture was carried out three times, and survived microorganisms were then seeded in agar with the same screening medium. Single colonies, which had been obtained one week later after the seeding, were collected and suspended in 50% glycerol for storage at below -80℃.
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