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2005 Fiscal Year Final Research Report Summary

Genetic oncological and molecular epidemiological research on development and progression of human bile duct cancer in Asia

Research Project

Project/Area Number 15406008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Pathological medical chemistry
Research InstitutionNagahama Institute of Bio-Science and Technology (2005)
University of Tsukuba (2003-2004)

Principal Investigator

MIWA Masanao  Nagahama Institute of Bio-Science and Technology, Faculty of Bioscience, Professor, バイオサイエンス学部, 教授 (20012750)

Co-Investigator(Kenkyū-buntansha) TODOROKI Takeshi  Univ.Tsukuba, Grad.Sch.Comp.Human Sci., Assoc.Prof., 大学院・人間総合科学研究科, 助教授 (70114105)
HIRANO Takashi  AIST, Inst.Biol.Resources Func., Principal Res.Scientist, 生物機能工学研究部門, 総括研究員 (90357864)
HANAI Shuji  AIST, Inst.Biol.Resources Func., Research Scientist, 特別研究員(研究職) (80333483)
SHODA Junichi  Univ.Tsukuba, Grad.Sch.Comp.Human Sci., Lecturer, 大学院・人間総合科学研究科, 講師 (90241827)
Project Period (FY) 2003 – 2005
KeywordsBiliary tract cancer / Thailand / India / Infection / liver fluke / Case control study / Polymorphism
Research Abstract

Taking advantage of collaboration with Dr.Petcharin Srivatanakul of National Cancer Institute of Thailand, we have successful cooperation with doctors and paramedical staffs in Nakorn Phanom Province, North East Thailand, and could perform a population-based case-control study of cholangiocarcinoma, in which sex, age and place of residence were matched individually. We could collect the peripheral blood and the information on the life-style related factors from the individuals who gave the informed consent.
We could confirm the liver fluke infection as the risk factor. In addition we found the drinking alcohol is another independent risk factor for cholangiocarcinoma. Past and current regular drinkers of alcohol had higher risk (odds ratio=5.39[95% CI:1.11-26.06] and 4.82[95% CI:1.29-18.06], respectively). Compared to subjects who had a normal antibody range and the wild type GSTM1 gene, those who had elevated antibodies had higher odds rations of 10.34(95% CI:1.31-81.63) for wild type GSTM1 and 18.00(95% CI:3.33-97.40) for the null variant thereof, respectively. We would like to collect more samples for the case-control study to confirm the above results in the near future.
On the biological characteristics of cholangiocarcinoma, we established a cholangiocarcinoma cell line, KKU 100, found that the excretory/secretory products from the liver fluke, Opisthorchis viverrini, could increase the cell proliferation of NIH-3T3 cells, and that MUC1 gene expression may be useful for predicting the poor outcome of the patients with intrahepatic cholangiocarcinoma.
In addition we also established a gall bladder cancer cell line and found that MUC1 gene expression as a prognostic factor in the patients with gall bladder cancer.

  • Research Products

    (18 results)

All 2005 2004 2003

All Journal Article (17 results) Book (1 results)

  • [Journal Article] Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line(KKU-100)2005

    • Author(s)
      Sropa, B., Miwa, M.et al.
    • Journal Title

      World J. Gastroenterol. 11(22)

      Pages: 3392-3397

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic and environmental determinants of risk for cholangiocarcinoma via Opisthorchis viverrini in a densely infested area in Nakhon Phanom, northeast Thailand2005

    • Author(s)
      Honjo, S., Miwa, M.et al.
    • Journal Title

      Int. J. Cancer 117(5)

      Pages: 854-860

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] MUC 1 core protein as a marker of gallbladder malignancy.2005

    • Author(s)
      Ghosh, M., Miwa, M.et ak.
    • Journal Title

      Eur. J. Surg. Oncol. 31(8)

      Pages: 891-896

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Musl and Muc5AC mucin expression in liver fluke-associated intrahepatic cholangiocarcinoma2005

    • Author(s)
      Boonla, C., Miwa, M.et al.
    • Journal Title

      World J. Gastroenterol. 11(32)

      Pages: 4939-4946

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (KKU-100).2005

    • Author(s)
      Sripa, B., Miwa, M.et al.
    • Journal Title

      World J.Gastroenterol. 11(22)

      Pages: 3392-3397

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetic and environmental determinants of risk for cholangiocarcinoma via Opisthorchis viverrini in a densely infested area in Nakhon Phanom, northeast Thailand.2005

    • Author(s)
      Honjo, S., Miwa, M.et al.
    • Journal Title

      Int.J.Cancer 117(5)

      Pages: 854-860

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] MUC 1 core protein as a marker of gallbladder malignancy.2005

    • Author(s)
      Ghosh, M., Miwa, M.et al.
    • Journal Title

      Eur.J.Surg.Oncol. 31(8)

      Pages: 891-896

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Muc1 and Muc5AC mucin expression in liver fluke-associated intrahepatic cholangiocarcinoma.2005

    • Author(s)
      Boonla, C., Miwa, M.et al.
    • Journal Title

      World J.Gastroenterol. 11(32)

      Pages: 4939-4946

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization and genetic analysis in the newly established human bile duct cancer cell lines.2005

    • Author(s)
      Ghosh, M., Miwa, M.et al.
    • Journal Title

      Int.J.Oncol. 26(2)

      Pages: 449-456

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection of Paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method.2005

    • Author(s)
      Intapan, P.M, Miwa, N.et al.
    • Journal Title

      Journal of Parasitology 91

      Pages: 195-198

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Evaluation of human IgG subclass antibodies in the serodiagnosis of paragonimiasis heterotremus.2005

    • Author(s)
      Wongkham, C., Miwa, M.et al.
    • Journal Title

      Pac.J.Allergy Immunol. 23

      Pages: 205-211

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Specific cancers in north Thailand : Liver cancer and lung cancer. (In Japanese)2005

    • Author(s)
      Miwa, N., Honjo, S, Nakachi, K.
    • Journal Title

      In : Forefront of Cancer Prevention (Tajima, K., Tokudome, S., Kono, S., Nakachi, K. eds.)(Showado, Kyoto, Japan)

      Pages: 151-157

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increased cell proliferation of mouse fibroblast NIH-3T3 in vitro induced by excertory/secretory product(s) from Opisthorchis viverrini2004

    • Author(s)
      Thuwajit, C., Miwa, M.et al.
    • Journal Title

      Parasitology 129(4)

      Pages: 455-464

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increased cell proliferation of mouse fibroblast NIH-3T3 in vitro induced by excretory/secretory product(s) from Opisthorohis viverrini.2004

    • Author(s)
      Thuwajit, C., Miwa, M.et al.
    • Journal Title

      Parasitology 129(4)

      Pages: 455-464

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Establishment and characterization of unique man gallbladder cancer cell lines.2004

    • Author(s)
      Ghosh, M., Miwa, M.et al.
    • Journal Title

      Int.J.Oncol. 24

      Pages: 1189-1196

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] infrequent microsatellite instability in liver fluke infection-associated intrahepatic cholangiocarcinomas from Thailand2003

    • Author(s)
      Liengswangwong, U., Miwa M. et al.
    • Journal Title

      Int. J. Cancer 107 (3)

      Pages: 375-380

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Infrequent microsatellite instability in liver fluke infection-associated intrahepatic cholangiocarcinomas from Thailand.2003

    • Author(s)
      Liengswangwong, U., Miwa, M. et al.
    • Journal Title

      Int.J.Cancer 107(3)

      Pages: 375-380

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] がん予防の最前線(下巻) (分担執筆) pp. 151-1572005

    • Author(s)
      三輪 正直, 本荘 哲, 中地 敬
    • Total Pages
      226
    • Publisher
      昭和堂
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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