2005 Fiscal Year Final Research Report Summary
Molecular and viral research on dengue hemorrhagic fever
| Project/Area Number |
15406020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Virology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MORITA Kouichi Nagasaki University, Institute of Tropical Medicine, Professor (40182240)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Shingo Nagasaki University, Institute of Tropical Medicine, Research Associate (00346925)
KUMATORI Atsushi Nagasaki University, Institute of Tropical Medicine, Assistant Professor (60244092)
TADANO Masayuki Ryukyu University, School of Medicine, Associate Professor (80179712)
TSUDA Yoshio Nagasaki University, National Institute of Infectious Disease, Chief (20207393)
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| Project Period (FY) |
2003 – 2005
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| Keywords | dengue hemorrhagic fever / molecular epidemiology / mosquito borne tropical disease / dengue virus / flow cytometry analysis |
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| Research Abstract |
In order to elucidate various virus and host factors that are relevant to the development of dengue hemorrhagic fever, we conducted a three-year research project. We isolated a number of dengue virus strains during dengue epidemic in Bangladesh, the Philippines, and Vietnam and determined nucleotide sequences of the virus strains. The data was compared with each other as well as the sequences obtained in conventional data base. We witnessed the genotype shift of dengue virus 2 in the Philippines during the early 2000s from local strain to cosmopolitan strain. While in Bangladesh and Vietnam, dengue viruses evolved locally and caused periodic epidemics in the countries. In the comparisons made with viruses from dengue hemorrhagic fever and dengue fever, we could not detect any significant differences between the two groups. In the flow cytometry analysis, we clearly demonstrated that dengue virus can infect peripheral B cells and NK cells suggesting the target of dengue virus in humans is not always on a particular cell group but the virus can choose the target cells according to its affinity to each virus group.
This idea was further strengthened later by the fact that two distinct virus strains were isolated from one patient by using different cell lines for isolation. One virus stain was isolated by mosquito cells and grow well in the cells but not human B cell line. While the other virus was isolated by K562, a culture cell line form human monocyte, grow well in B cell lines. Those two viruses possessed an amino acid alternation in E protein, suggesting a determinant of cell tropism. It is very likely that dengue virus population gap may have some effect to determine dengue virus pathogenesis in human infection. Further clarification of the importance of this dengue virus population gap is necessary to understand the mechanism to determine the severity in dengue virus infections in humans.
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[Journal Article] Molecular Characterization and Clinical Evaluation of Dengue Outbreak in 2002 in Bangladesh2006
Author(s)
Islam M.A., Ahmed M.U., Begum N., C howdhury N.A., Khan A.F., Parquet M.C, Bipolo S., Inoue S., Hasebe F., Suzuki Y., Morita K.
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Journal Title
Japanese Journal of Infectious Diseases 59
Pages: 85-91
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Identification and Characterization of Carbohydrate Molecules in Mammalian Cells Recognized by Dengue Virus Type 2.2006
Author(s)
Aoki, C., HidariK.IPJ., Itonori, S., Yarnada, A., Takahashi, N.Kasama, T., Hasebe, F., Islam, MA., Hatano, K., Matsuoka, K., Taki, T., Guo, C-T., Takahashi, T., Sakano, Y., Suzuki, T., Miyamoto, D., Sugita, M., Terunuma, D., Morita, K., Suzuki, Y.
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Journal Title
Journal of Biochemistry Vol.139
Pages: 6007-614
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification and characterization of carbohydrate determinant that is recognized with domain III of dengue virus envelope glycoprotein.2006
Author(s)
Hidari K., Aoki C., Yamada A., Hasebe F., Islam MA., Hatano K., Matsuoka K., Guo CT., Takahashi T., Sakano Y., Suzuki T., Miyamoto D., Terumura D., Morita K., Suzuki Y.
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress.
Place of Presentation
Kyoto, Japan
Year and Date
20060618-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] TNF-α promoter polymorphism and DHF in Vietnam.2004
Author(s)
Hirayama K., Kikuchi M., Huong V. T. Q., Ngu V. T. T., Tham V. D., Dat T. V., Ha D. Q., Morita K.
Organizer
Nagasaki Symposium on Tropical Emerging Infectious Diseases and JSPS Workshop on Infectious Diseases in Vietnam.
Place of Presentation
Nagasaki, Japan
Year and Date
20041125-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections.2004
Author(s)
Oishi K., Saito M., Inoue S., Dimaano E. M., Alera M. T. P., Robles M. P., Estrella, Jr. B. D., Kumatori A., Moji K., Alonzo B. M., Buerano C. C., Matias R. R., Morita K., Natividad F. F., Nagatake T.
Organizer
Nagasaki Symposium on Tropical Emerging Infectious Diseases and JSPS Workshop on Infectious Diseases in Vietnam.
Place of Presentation
Nagasaki, Japan
Year and Date
20041125-27
Description
「研究成果報告書概要(欧文)」より
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