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2005 Fiscal Year Final Research Report Summary

An evaluation of anti-VacA antibody and its target molecules in the cerebrospinal fluid of patients with demyelinating form of Guillain-Barre syndrome.

Research Project

Project/Area Number 15500242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionSapporo Medical University

Principal Investigator

CHIBA Susumu  Sapporo Medical University, Department of Neurology, School of Medicine, Associate Professor, 医学部, 助教授 (30167514)

Co-Investigator(Kenkyū-buntansha) SUGIYAMA Toshiroh  Toyama University, The 3rd Department of Internal Medicine, School of Medicine, Professor, 医学部, 教授 (00196768)
IMAI Tomihiro  Sapporo Medical University, Department of Neurology, School of Medicine, Assistant Professor, 医学部, 講師 (40231162)
NONAKA Michio  Sapporo Medical University, Department of Neurology, School of Medicine, Instructor, 医学部, 助手 (50363685)
Project Period (FY) 2003 – 2005
KeywordsHelicobacter pylori / anti-VacA antibody / cerebrospinal fluid / Guillain-Barre syndrome / molecular homology / 分子相同性
Research Abstract

Immunological study
In order to detect antibodies against recombinant vacuolating cytotoxin (r-VacA) of Helicobacter pylori (H.pylori) in the cerebrospinal fluid (CSF) from patients with Guillain-Barre syndrome (GBS). The CSF samples from 13 patients with GBS (electrophysiologically classified as 8 acute inflammatory demyelinating polyradiculoneuropathy (AIDP), 4 acute motor axonal neuropathy (AMAN), and 1 unexcitable) and 8 disease control patients were studied. The r-VacA protein was separated by SDS-PAGE and Western blot analysis was carried out. Six of the 13 GBS patients had a specific IgG antibody against VacA of H.pylori, which was confirmed by absorption experiments using r-VacA. Every patient with positive CSF anti-r-VacA antibody had AIDP. The sequence homology between VacA and human (Na^++K^+)-ATPase α subunit has been outlined. Our findings lead to the possible hypothesis that anti-VacA antibodies involve ion channels in abaxonal Schwann cell plasmalemma resulting in demyeli … More nation in some patients with GBS.
Histochemical evaluation
To identify the target molecules of anti-VacA-antibody (A-VacA-Ab) in the human ventral nerve roots, the immunohistochemical examination was carried out. A-VacA-Ab obtained from rabbits was prepared by immunization of the native VacA purified by the culture media of H.pylori. Cervical ventral nerve roots were obtained from patients with no known neurologic disorders within two hours after death. Both DAB and FITC procedures were employed. Disseminated spotty stainings were observed in cross sections. In longitudinal sections, string-like immunostainings were also seen both by DAB and FITC procedures. These were localized within the nerve fibers. Consequently, the target epitopes of A-VacA-Ab were thought to be present in the myelin components of the nerve roots. These results indicate that the target molecules of A-VacA-Ab in the cerebrospinal fluid obtained from patients with GBS are probably associated with some components of the peripheral nerve myelin. A-VacA-Ab may play an important role in the immune responses of patients with the demyelinating form of GBS. Less

  • Research Products

    (10 results)

All 2006 2005 2004 2003

All Journal Article (10 results)

  • [Journal Article] Autoantibodies against HSP7O family proteins in CSF of multiple sclerosis; :.2006

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Fujii N, Matsumoto H.
    • Journal Title

      J Neurol Sci 241

      Pages: 39-43

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Autoantibodies against HSP70 family proteins in CSF of multiple sclerosis.2006

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Fujii N, Matsumoto H.
    • Journal Title

      J Neurol Sci 241

      Pages: 39-43

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An evaluation of immunological cross-reactvity between Vac-A of Helicobacter pylori and myelin P0 protein using the cerebrospinal fluid from patients with Guillain-Barre syndrome.2005

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Furuyama H, Okabayashi T, Matsumoto H, Fujii N.
    • Journal Title

      Helicobacter 10

      Pages: 514

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An antibody to VacA of Helicobacter pylori in the CSF of patients with Miller-Fisher syndrome.2004

    • Author(s)
      Chiba S, Sugiyama T, Yonekura K, Tanaka S, Matsumoto H, Fujii N, Yokota S, Hirayama T.
    • Journal Title

      Neurology 63

      Pages: 2184-2187

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prevalence of anti-heat shock protein in cerebrospinal fluids of patients with Guillain-Barre syndrome.2004

    • Author(s)
      Yonekura K, Yokota S, Tanaka S, Kubota H, Fujii N, Matsumoto H, Chiba S.
    • Journal Title

      J Neuroimmunol 156

      Pages: 204-209

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Localization and function in ER stress tolelance of Erdj3, a new member of HSP4O family protein.2004

    • Author(s)
      Nakanishi K, Kamiguchi K, Torigoe T, Nabeta C, Hirohashi Y, Asanuma H, Tobioka H, Koge N, Harad IO, Ichimiya S, Noagno H, Yano S, Chiba S, Matsumoto H, Sato N.
    • Journal Title

      Cell stress and Chaperone 9

      Pages: 253-264

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 中毒性・薬剤性ニューロパチー2004

    • Author(s)
      千葉 進, 田中真悟, 古山裕康
    • Journal Title

      Medicina 41

      Pages: 1329-1331

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Helicobacter pylori 感染と神経疾患.2003

    • Author(s)
      千葉 進, 田中真悟
    • Journal Title

      Helicobacter Research 7

      Pages: 327-331

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structures, biological activities and antigenic properties of Helicobacter pylori lipopolysaccharide.2003

    • Author(s)
      Yokota S, Amano K, Chiba S, Fujii N.
    • Journal Title

      Recent Reserch Developments in Microbiology 7

      Pages: 251-267

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Antibodies against VacA of Helicobacter pylon can recognize the epitopes of the human peripheral nerve myelin protein.2003

    • Author(s)
      Chiba S, Sugiyama T, Matsumoto H, Yonekura K, Tanaka S et al.
    • Journal Title

      Helicobacter 8(4)

      Pages: 445

    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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