2004 Fiscal Year Final Research Report Summary
Regulation of channel activities by glycosylation during acquirement of neural plasticity
| Project/Area Number |
15500260
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Osaka Prefecture University (2004)
Nara Institute of Science and Technology (2003) |
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Principal Investigator |
KATO Keiko Osaka Prefecture University, Graduate School of Life and Environmental Sciences, Associate professor, 大学院・農学生命科学研究科, 助教授 (90252684)
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| Project Period (FY) |
2003 – 2004
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| Keywords | sialylation / neural plasticity / raft / glycosyltransferase / channel / epilepsy / knock-out |
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| Research Abstract |
I have proposed a hypothesis that diversity of neural plasticity is regulated by post-translational modification of proteins. I have focused on sialylated glycoproteins in brain and have investigated on sialylation in neural plasticity in vivo and in vitro. We have found that kindling stimulation of mice induced acquirement of structural neural plasticity and the acquirement induced increase of a α2,3-sialyltransferase expressions in the hippocampus and the anterior thalamic nuclei which receive projects from the hippocampus. As the α2,3-sialyltransferase is a key enzyme for sialylation of several channels, we planed to investigate on effects of the enzyme on how channel functions are correlated with changes of structural neural plasticity. First, we prepared conditional knock-out mice against the enzyme gene in this grand. During two years, we finished to make the model mice. At the same time, second, we planed to examine membrane dynamics between rafts and synapses for understanding effects of sialylation on channel functions. During this grand, we determined conditions to separate from raft to synapse membranes and we found different glycan forms on several channels between raft and synapse membranes. This grand gave a chance that I start to investigate membrane dynamics of channels in α2,3-sialyltransferase deficient mouse brain.
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[Journal Article] Role of loop structures of neuropsin in activity of serine protease and regulated secretion.2002
Author(s)
Oka T., Hakoshima, T., Itakura, M., Yamamori, S., Takahashi, M., Hashimoto, Y., Shiosaka S., Kato, K.
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Journal Title
J Biol Chem 277
Pages: 14724-14730
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Extracellular serine protease neuropsin (KLK8) modulates neurite outgrowth and fasciculation of mouse hippocampal neurons in culture.2002
Author(s)
Oka, T., Akisada, M., Okabe, A., Sakurai, K., Shiosaka, S., Kato, K.
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Journal Title
Neurosci.Lett 321
Pages: 141-144
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serine proteinase inhibitor 3 and murinoglobulin I are potent inhibitors of neuropsin in adult mouse brain.2001
Author(s)
Kato, K., Kishi, T., Kamachi T., Akisada, M., Oka, T., Midorikawa, R., Takio, K., Dohmae, N., Bird, P.I., Sun, J., Scott, F., Miyake, Y., Yamamoto, K., Machida, A., Tanaka, T., Matsumoto, K., Shibata, M., Shiosaka, S.
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Journal Title
J Biol Chem 276
Pages: 14562-14571
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Differential expression of mRNAs for sialyltransferase isoenzymes induced in the hippocampus of mouse following kindled-seizures.2001
Author(s)
Okabe, A., Tawara, Y., Masa, T., Oka, T., Machida, A., Tanaka, T., Matsuhashi, H., Shiosaka, S., Kato, K.
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Journal Title
J Neurochem 77
Pages: 1-14
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Dendritic aberrations in the hippocampal granular layer and the amygdalohippocampal area following kindled-seizures.2001
Author(s)
Kato, K., Masa, T., Tawara, Y., Kobayashi, K., Oka, T., Okabe, A., Shiosaka, S.
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Journal Title
Brain Res 901
Pages: 281-295
Description
「研究成果報告書概要(欧文)」より
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