2004 Fiscal Year Final Research Report Summary
Severe Combined Immunodeficient(SCID) Mice As Animal Models for Human Echinococcosis
| Project/Area Number |
15500295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
NAKAYA Kazuhiro Asahikawa Medical College, Associate Professor, 医学部, 助教授 (70109388)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASAKI Hiroshi Asahikawa Medical College, Associate Professor, 医学部, 助教授 (00138207)
NAKAO Minoru Asahikawa Medical College, Associate Professor, 医学部, 助手 (70155670)
SAKO Yasuhito Asahikawa Medical College, Associate Professor, 医学部, 助手 (40312459)
ITO Akira Asahikawa Medical College, Professor, 医学部, 教授 (70054020)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Immunodeficient (SCID) mouse / NOD / Shi-scid / Echinococcus multilocularis / metacestode / intermediate host / Animal model / multivesiculation / protoscolex formation |
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| Research Abstract |
At first, two strains of sever combined immune-deficiency mice, C.B-17/Icr-scid and NOD/Shi-scid were compared with the sensitivity to Echinococcus multilocularis metacestode. At post infection 9 and 12weeks, the developed metacestode of two strains infra peritoneal cavities were weighed and compared. NOD/Shi-scid showed more high degree weight than C.B-17/Icr-scid at both points of weeks. It was suggested that scid-gene, declines of macrophage function and compliment activity were influenced together.
So, development and growth of metacestode in NOD/Shi-scid were observed in detail. At post infection 1,2,3 weeks, between NOD/Shi-scid and control NOD/Shi-+/+ mice, there were no defferences both weight and morphologies of metacestodes. NOD/Shi-+/+ mice were not revealed for rEm-18 antibody in these periods. It was considered that so-called prepatent period which antibody was not yet producted in host immune system. After post infection 6 weeks, in NOD/Shi-scid mice, multivesiculation and formation of protoscolex were rapidly proceeded. NOD/Shi-scid mice showed striking torelance for the parasite development and growth. It was suggested that NOD/Shi-scid mouse would be a model for human echinococcosis.
And moreover, it was attempted of quantification of multivesiculation and protoscolex formation of the parasite between NOD/Shi-scid and NOD/Shi-+/+ mice. After post infection 6 weeks, in NOD/Shi-scid mice, index of multivesiculation and protoscolex formation suddenly increased. While in NOD/Shi-+/+ mice, in spite of spread of parasite and inflamated fucus, index of multivesicelation was decreased for not changed number of vesicular. Index of protoscolex also increased more in NOD/Shi-scid mice than in NOD/Shi-+/+ mice. It was expected that index methods of these parasite development or growth was usefullness in evaluation of host sensitivity and drug efficacy, estimate of infectious point.
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Research Products
(36 results)
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[Journal Article] Echinococcus multilocularis confirmed on Kunashiri Island, 15kilometers from the eastern Part of Hokkaido, Japan.2005
Author(s)
Satoh M, Nakaya K, Nakao M, Xiao N, Yamasaki H, Sako Y, Naitoh Y, Kondo S, Kobayashi M, Ohtaishi N, Ito A.
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Journal Title
Am J Trop Med Hyg. 72・3
Pages: 284-288
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Echinococcus multilocularis confirmed from Kunashiri Island, 15km far from Hokkaido, Japan2005
Author(s)
Satoh M, Nakaya K, Nakao M, Xiao N, Yamasaki H, Naitoh Y, Kondo S, Kobayashi M, Ohtaishi N, Ito A.
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Journal Title
Am J Trop Med Hyg. 72(3)
Pages: 284-288
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular Cloning, Expression, and Serological Evaluation of an 8-Kilodalton Subunit of Antigen B from Echinococcus multilocularis.2004
Author(s)
Mamuti W, Yamasaki H, Sako Y, Nakao M, Xiao N, Nakaya K, Sato N, Vuitton DA, Piarroux R, Lightowlers MW, Craig PS, Ito A.
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Journal Title
J Clin Microbiol. 42・3
Pages: 1082-1088
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Molecular Cloning, Expression, and Serological Evaluation of an 8-Kilodalton Subunit of Antigen B from Echinococcus multilocularis.2004
Author(s)
Mamuti W, Yamasaki H, Sako Y, Nakao M, Xiao N, Nakaya K, Sato N, Vuitton DA, Piarroux R, Lightowlers MW, Craig PS, Ito A.
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Journal Title
J Clin Microbiol. 42(3)
Pages: 1082-1088
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections.2003
Author(s)
Xiao N, Mamuti W, Yamasaki H, Sako Y, Nakao M, Nakaya K, Gottstein B, Schatz PM, Lihtowlers MW, Craig PS, Ito A.
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Journal Title
Journal of Clinical Microbiology 41・7
Pages: 3351-3353
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Multiple genotypes of Taenia solium-ramifications for diagnosis, treatment and control.2003
Author(s)
Ito A, Yamasaki H, Nakao M, Sako Y, Okamoto M, Sato MO, Nakaya K, Margono SS, Ikejima T, Kassuku AA, Afonso SM, Ortiz WB, Plancarte A, Zoli A, Geerts S, Craig PS.
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Journal Title
Acta Trop. 87・1
Pages: 95-101
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Taenia solium taeniasis/cysticercosis in Papua, Indonesia in 2001 : detection of human worm carriers.2003
Author(s)
Margono SS, Ito A, Sato MO, Okamoto M, Subahar R, Yamasaki H, Hamid A, Wandra T, Purba WH, Nakaya K, Ito M, Craig PS, Suroso T.
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Journal Title
J Helminthol. 77・1
Pages: 39-42
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine : usefulness of ELISA using purified glycoproteins and recombinant antigen.2003
Author(s)
Sato MO, Yamasaki H, Sako Y, Nakao M, Nakaya K, Plancarte A, Kassuku AA, Dorny P, Geerts S, Benitez-Ortiz W, Hashiguchi Y, Ito A.
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Journal Title
Vet Parasitol. 111・4
Pages: 309-322
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections.2003
Author(s)
Xiao N, Mamuti W, Yamasaki H, Sako Y, Nakao M, Nakaya K, Gottstein B, Schatz PM, Lightowlers MW, Craig PS, Ito A.
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Journal Title
Journal of Clinical Microbiology 41(7)
Pages: 3351-3353
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Multiple genotypes of Taenia solium-ramifications for diagnosis, treatment and control.2003
Author(s)
Ito A, Yamasaki H, Nakao M, Sako Y, Okamoto M, Sato MO, Nakaya K, Margono SS, Ikejima T, Kassuku AA, Alonso SM, Ortiz WB, Plancarte A, Zoli A, Geerts S, Craig PS.
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Journal Title
Acta Trop. 87(1)
Pages: 95-101
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Taenia solium taeniasis/cysticercosis in Papua, Indonesia in 2001 : detection of human worm carriers.2003
Author(s)
Margono SS, Ito A, Sato MO, Okamoto M, Subahar R, Yamasaki H, Hamid A, Wandra T, Purba WH, Nakaya K, Ito M, Craig PS, Suroso T.
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Journal Title
J Helminthol. 77(1)
Pages: 39-42
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine : usefulness of ELISA using purified glycoproteins and recombinant antigen.2003
Author(s)
Sato MO, Yamasaki H, Sako Y, Nakao M, Nakaya K, Plancarte A, Kassuku AA, Dorny P, Geerts S, Benitez-Ortiz W, Hashiguchi Y, Ito A.
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Journal Title
Vet Parasitol. 111(4)
Pages: 309-322
Description
「研究成果報告書概要(欧文)」より
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