2004 Fiscal Year Final Research Report Summary
Analysis of DNA vaccine delivery system using attenuated strains of Salmonella typhimurium.
| Project/Area Number |
15500301
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
HAGA Takeshi University of Miyazaki, Agriculture, Associate Professor, 農学部, 助教授 (20315360)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Yoshitaka University of Miyazaki, Agriculture, Professor, 農学部, 教授 (30142136)
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| Project Period (FY) |
2003 – 2004
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| Keywords | DNA vaccine / delivery / intracellular bacteria / immune response |
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| Research Abstract |
Abilities of live attenuated Salmonella typhimurium(S.typhimurium) as a carrier of DNA vaccine were evaluated using model plasmid encoding beta-galactosidase(b-gal) and BALB/c mice. Expression of beta-galactosidase was occasionally observed in macrophage-like cells from pCMVbeta delivered by two strains (CR11 derivered pho P and 2337-65 derivative SL7207) of S.typhimurium. During the experiments, pCMVbeta was found to be lost in S.typhimurium. To stabilize plasmid in S.typhimurium, pBRCMVbeta having a replication origin from pBR322 was constructed. Although pBRCMVbeta remained stable in S.typhimurium, no evidence for the expression of b-gal was obtained from the infection of S.typhimurium bearing pBRCMVbeta. No b-gal specific IgG was observed in mice orally administrated with S.typhimurium having pBRCMVbeta or pCMVbeta. These data suggest that the mechanism needs to be further elucidated to rationally improve the system.
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