2005 Fiscal Year Final Research Report Summary
Bone and skin regeneration therapy with bone marrow mesenchymal cells
| Project/Area Number |
15500319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Nara Medical University |
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Principal Investigator |
YOSHIKAWA Takafumi Nara Medical University, Medicine, Lecturer, 医学部, 講師 (90275347)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Yoshinori Nara Medical University, 医学部, 教授 (40094578)
KAWATE Kenji Nara Medical University, 医学部, 講師 (70275329)
OHMURA Tetsuji Nara Medical University, 医学部, 助手 (20332968)
UEDA Yurito Nara Medical University, 医学部, 助手 (70203453)
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| Project Period (FY) |
2003 – 2005
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| Keywords | bone / skin / marrow cell / regeneration / Dexamethasone / estriol / therapy |
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| Research Abstract |
Autogenous bone graft is ideal from the point of view of rejection and osteogenic potential, and also produces the best results clinically. When pseudoarthrosis occurs due to poor union of a bone fracture, cancellous bone with high cellular activity is generally harvested from the ilium and then grafted to the affected area to facilitate bone union. However, autogenous bone cannot be used to reconstruct extensive bone defects, as there is naturally a limit to the amount that can be harvested. This method also has the drawback of causing damage to normal bone and soft tissue. Harvesting cancellous bone from the ilium is associated with severe postoperative pain, and patients experience more pain at the harvest site than at the graft site, thus resulting in poor patient satisfaction. Management of pain at the harvest site is often difficult for patients and medical professionals. There have also been reports of pelvic deformation and persistent pain lasting more than one year after surge
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ry. Autograft skin flap operation is also invasive and unsuitable for old patients. If a tissue engineering approach was used to produce autogenous bone or skin ex vivo or with culture techniques, extensive skin or bone tissue defects could be repaired without damaging normal tissues.
Bone marrow cells include hemopoietic cells and cells with an bone or skin tissue regeneration capacity. However, the ability of human marrow cells is unreliable. We have developed the culture techniques of human marrow mesenchymal cells to enhance its tissue regeneration ability. We have experienced more than 35 cases who were treated using our tissue engineering techniques. In all patients, autologous marrow mesenchymal cell transplantation was shown to be therapeutically effective, and there were no adverse reactions associated with transplantation. With present type of tissue-engineered bone or skin, an artificial material with a high tissue regeneration capacity can be prepared by aspiration, which is minimally invasive, and thus when compared to autografts, it is possible to radically reduce postoperative pain without damage of normal tissue. Less
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