2004 Fiscal Year Final Research Report Summary
Study on the prediction of thrombosis formation during exercise
| Project/Area Number |
15500455
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Nara Medical University |
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Principal Investigator |
ISHIZASHI Hiromichi Nara Medical University, Faculty of Medicine, Assistant Professor, 保健体育学, 講師 (50260807)
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| Co-Investigator(Kenkyū-buntansha) |
松本 雅則 奈良県立医科大学, 輸血部, 助手 (60316081)
藤村 吉博 奈良県立医科大学, 輸血部, 教授 (80118033)
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| Project Period (FY) |
2003 – 2004
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| Keywords | VWF / UL-VWFM / ADAMTS13 / hard exercise / mild exercise / sweating / dehydration / thrombi formation |
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| Research Abstract |
von Willebrand factor(VWF) is synthesized in vascular endothelial cells, and released into the plasma as "unusually large" VWF multimers(UL-VWFM), which is degraded by ADAMTS13. Deficiency of ADAMTS13 increases the plasma levels of UL-VWFM, which leads to platelet clumping and/or thrombi formation. VWF antigen(VWF:Ag) in plasma was reported to increase during exercise, but the dynamics on VWT and its degradation on exercise has not been clarified. In order to clarify their homeostatic modulation in vivo, we measured platelet count and plasma values of VWF:Ag, UL-VWFM, ristocetin cofactor(RCof) and ADAMTS13 before and after exercise for 12 hours in 20 healthy male volunteers. Two types of exercise was applied using bicycle ergometer as follows ; 65% VO2 max for 1 hour (hard exercise) and 40% VO2 max for 1 four (mild exercise). As a result, immediately after hard exercise, VWF:Ag and RCof significantly increased, and UL-VWFM was detected, and lasted for 6 hours and disappeared in 12 hour
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s. In contrast, after mild exercise, the increase in VWF was not observed. ADAMTS13 significantly decreased 2 hours after hard exercise and gradually recovered within 5 hours. These findings suggest that the increase in UL-VWFM after hard exercise is due to the compensatory phenomenon to prevent the excessive bleeding at trauma caused by exercise-induced hypertension, followed by the consumption of ADAMTS13 to degrade increased amounts of UL-VWFM. Furthermore, we evaluated the relationship of VWF:antigen to dehydration during exercise because of sweating. As a result, RCof after hard exercise tended to be positively correlated with the degree of sweating per body weight, supporting that the degree of dehydration is closely related to the ability of VWF-dependent platelet aggregation during exercise. These results suggest that enough amount of water intake will be necessary to obtain early physiological response to decrease the platelet aggregation and subsequent occurrence of thrombi formation in small vessels at the recovery phase after exercise. Less
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Research Products
(11 results)
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[Journal Article] Molecular characterization of ADAMTS13 gene mutations in Japanese patients with Upshaw-Schulman syndrome.2004
Author(s)
Matsumoto M, Kokame K, Soejima K, Miura M, Hayashi S, Fujii Y, Iwai A, Ito E, Tsuji Y, Takada-Shitaka M, Iwadate M, Umeyama H, Yagi H, Ishizashi H, Banno F, Nakagaki T, Miyata T, Fujimura Y.
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Journal Title
Blood 103
Pages: 1305-1310
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of novel mutations in ADAMTS13 in an adult patient with congenital thrombotic thrombocytopenic purpura.2004
Author(s)
Uchida T, Wda H, Mizutani M, Iwashita M, Ishihara H, Shibano T, Suzuki M, Matsubara Y, Soejima K, Matsumoto M, Fujimura Y, Ikeda Y, Murata M.
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Journal Title
Blood 104
Pages: 2081-2083
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Significance of measuring von Willebrand factor-cleaving protease activity in thrombotic microangiopathy after living donor liver transplantation.2003
Author(s)
Nakazawa Y, Hashikura Y, Ogino S, Urata K, Ikegami T, Terada M, Yagi H, Ishizashi H, Matsumoto M, Fujimura Y, Kawasaki S.
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Journal Title
Liver Transplant 9
Pages: 1328-1333
Description
「研究成果報告書概要(欧文)」より
