2005 Fiscal Year Final Research Report Summary
Study on the degradation and control of endocrine disruptors by cytochrome P450-producing bacteria.
| Project/Area Number |
15510074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | KIBI International University (2004-2005)
Okayama University (2003) |
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Principal Investigator |
IKATSU Hisayoshi KIBI International University, Department of Environmental Risk Management, School of Policy Management, Professor, 政策マネジメント学部, 教授 (10260663)
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| Project Period (FY) |
2003 – 2005
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| Keywords | cytochrome P450 / endocrine disruptors / biodegradation / 2-ethoxyphenol / phthalate esters |
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| Research Abstract |
Cytochrome P450s are widely distributed in the biosphere and catalyze a remarkably broad range of chemical reactions on an equally broad range of substrates. Microorganisms are decomposers in the ecosystem and act to alleviate environmental pollution by degrading pollutants. Therefore, the bacteria producing cytochrome P450 are good candidates for biodegradation of endocrine disruptors. In this research the degradation ability of endocrine disruptors by cytochrome P450-producing bacteria, Rhodococcus sp.strain EPI and Gordonia sp.strain EP4, was investigated.
Strains EP1 and EP4 cultivated in M9 medium containing 2-ethoxyphenol, a P450-inducer, as a sole carbon source degraded di-n-butyl phthalate, di-n-propyl phthalate and diallyl phthalate. The cell-free extract of strain EP1 had metabolizing activity to DDT in addition to phthalate esters. On the other hands, the cell-free extract of strain EP4 had not metabolizing activity to DDT. The purified P450s from strain EP1 and EP4 metabolized not only phthalate esters but also 1,1,1-trichloroethane, carbon tetrachloride, benzene and toluene.
In this research we demonstrated that P450-producing bacteria wide distributed in the environment and the P450 participated in decomposition of endocrine disrupters. A possibility exists that the bacterial P450s are potential tools in the control of endocrine disruptors in environment.
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