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2004 Fiscal Year Final Research Report Summary

Hydration property of lipid bilayers composed of sphingomyelins having functional groups forming hydrogen bonds with water molecules.

Research Project

Project/Area Number 15550133
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional materials chemistry
Research InstitutionOkayama University of Science

Principal Investigator

KODAMA Michiko  Okayama University of Science, Professor, 理学部, 教授 (40101282)

Co-Investigator(Kenkyū-buntansha) OHIRA Susumu  Okayama Uninersity of Science, Professor, 理学部, 教授 (10194284)
AOKI Hyrouki  Okayama University of Science, Associate Professor, 理学部, 助教授 (80248202)
Project Period (FY) 2003 – 2004
KeywordsSphingomyelins / lipid bilayer / lipid microdomein / interdigitation packing / interlamellar water / differential scanning calorimetry / X-ray diffraction
Research Abstract

Recently, a new concept has been proposed for a lipid bilayer structure in biomembranes. In this connection, much attention of many investigators has been paid to lipid rafts and/or lipid caveolas. In this accord, we started our studies of bilayer packing of sphingomyelins, the most typical sphingophospholipid in biomembranes. Naturally occurring sphingomyelins are characterized by a widely distributed chain-length for the amide-linked fatty acid. In this study, the hydration property of bovine brain sphingomyelin was investigated by a deconvolution method of ice-melting differential scanning calorimetry (DSC) curves of varying water contents. For comparison, N-palmitoylsphingomyelin (C16:0-SM) having a homogeneous amide-linked fatty acid chain was partially synthesized by deacylation-reacylation of naturally occurring brain sphingomyelin and the final product was purified with sillcic acid callum chromatography. For the semisynthetic sphingomyelin, the ice-melting DSC curves were meas … More ured with the same way as that for the naturally occurring brain sphingomyelin. Results obtained in the present study are as follows : (1)The number of nonfreezable interlamellar water molecules is the same, 6 H2O/lipid, for the naturally occurring brain and the semisynthetic sphingomyelins ; (2)The number of freezable interlamellar water molecules is larger by two times for the naturally occurring brain than for the semisynthetic sphingomyelins ; (3)the naturally occurring brain sphingomyelins, which have a longer amide-linked fatty acid chain compared with their sphingosine chain (C-18), pack in an interdigitation form in an intrabilayer, so that the surface of bilayers is not flat, thus, resulting in a concavo-convex surface forming a water pool between bilayers ; (4)The semisynthetic sphingomyelin forms a flat surface between bilayers ; and (5)On the basis of the flat and/or concavo-convex surface, the amount of water molecules incorporated into regions between bilayer becomes different and is larger for the naturally occurring brain sphingomyelin than for the semisynthetic sphingomyelin. Less

  • Research Products

    (12 results)

All 2005 2004 2003 Other

All Journal Article (12 results)

  • [Journal Article] Unilamellar Vesicle-Forming Property of N-Nervonoylsphingomyelin (C24:1-SM) as Studied by Differential Scanning Calorimetry and Negative Stain Electron Microscopy2005

    • Author(s)
      Y.Kawasaki
    • Journal Title

      Thermochim.Acta 431

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Water Behavior in the Conversion process of Gel to Subgel Phase in Dimyristoylphosphatidylethanolamine-Water System as Studied by DSC2005

    • Author(s)
      H.Aoki
    • Journal Title

      Thermochim.Acta 431

      Pages: 6

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Powerful Property of N-nervonoylsphingomyelin (C24:1-SM) for the Formation of unilameliar vesicles as studied by Microcalorimetry and eElectron Microscopy2005

    • Author(s)
      Y.Kawasaki
    • Journal Title

      Biochim.Biophys.Acta (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Water Behavior in the Conversion process of Gel to Subgel Phase in Dimyristoylphosphatidylethanolamine-Water System as Studied by DSC.2005

    • Author(s)
      H.Aoki, M.Kodama
    • Journal Title

      Thermochim.Acta 431

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Unilamellar Vesicle-Forming Property of N-Nervonoylsphingomyelin (C24 : 1-SM) as Studied by Differential Scanning Calorimetry and Negative Stain Electron Microscopy.2005

    • Author(s)
      Y.Kawasaki, T.Tokura, A.Kuboki, S.Ohira, M.Kodama
    • Journal Title

      Thermochim.Acta 431

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Estimation of Interlamellar Water Molecules in Sphingomyelin Bilayer Systems Studied by DSC and X-ray diffraction.2004

    • Author(s)
      M.Kodama
    • Journal Title

      Thermochim.Acta 416

      Pages: 105-111

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Components and Fractions for Differently Bound Water Molecules of Dipalmitoylphosphatidylcholine -Water System as Studied by DSC and ^2H-NMR Spectroscopy2004

    • Author(s)
      M.Kodama
    • Journal Title

      Biochim.Biophys.Acta 1667

      Pages: 55-66

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Estimation of Interlamellar Water Molecules in Sphingomyelin Bilayer Systems Studied by DSC and X-ray diffraction.2004

    • Author(s)
      M.Kodama, M.Abe, Y.Kawasaki, K.Hayashi, S.Ohira, H.Nozaki, C.Katagiri, K.Inoue, H.Takahashi
    • Journal Title

      Thermochim.Acta 416

      Pages: 105-111

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Components and Fractions for Differently Bound Water Molecules of Dipalmitoylphosphatidylcholine-Water System as Studied by DSC and ^2H-NMR Spectroscopy.2004

    • Author(s)
      M.Kodama, Y.Kawasaki, H.Aoki, Y.Furukawa
    • Journal Title

      Biochim.Biophys.Acta 1667

      Pages: 55-66

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hydration Properties of Lipid Bilayer Systems Studied Using DSC And ^2H-NMR Spectroscopy2003

    • Author(s)
      M.Kodama
    • Journal Title

      Recent Res.Devel.Chem.Phys.Lipids 1

      Pages: 85-98

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hydration Properties of Lipid Bilayer Systems Studied Using DSC and ^2H-NMR Spectroscopy.2003

    • Author(s)
      M.Kodama, Y.Kawasaki, H.Aoki, E.Yamamoto, Y.Furukawa
    • Journal Title

      Recent Res.Devel.Chem.Phys.Lipids 1

      Pages: 85-98

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Powerful Property of Unsaturated N-Nervonoylsphingomyelin (C24 : 1-SM) for the Formation of Unilamellar Vesicles as Studied by Microcalorimetry and Electron Microscopy

    • Author(s)
      Yuko Kawasaki, Hiroko Nishikido, Atuhito Kuboki, Susumu Ohira, Michiko Kodama
    • Journal Title

      Biochim.Biophys.Acta (In Press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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