2005 Fiscal Year Final Research Report Summary
Molecular mechanisms regulating ethylene biosynthesis in plant
| Project/Area Number |
15570029
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
植物生理・分子
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
SATO Takahide Chiba Univ., Graduate School of Science and Technology, Professor, 大学院・自然科学研究科, 教授 (60125929)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Keywords | Ethylene / ACC synthase / AP2 / ERF family / Cucumis melo (melon) |
|---|
| Research Abstract |
Ethylene is a gaseous plant hormone that controls many aspects of plant growth and development. 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase are key enzymes in ethylene biosynthesis pathway in higher plants. A melon 1-aminocyclopropane-1-carboxylate synthase (CMe-ACS2) is a key enzyme in the ethylene biosynthesis pathway in melon, but mechanisms regulating CMe-ACS2 expression remain to be clarified. We characterized three melon AP2/ERF transcription factors (CMe-DREB1, CMe-ERF1 and CMe-ERF2), which were isolated by a yeast one-hybrid screening method using a GCCGAC sequence (DRE/CRT cis-acting element) in the CMe-ACS2 promoter. We demonstrated that CMe-DREB1 strongly bound to the GCCGAC sequence, but CMe-ERF1 and CMe-ERF2 did not. Transient expression analysis showed that only CMe-DREB1 could activate the transcription of the downstream reporter gene through the GCCGAC sequence in melon leaf cells. The expression levels of CMe-ACS2 and CMe-DREB1 in melon leaves were increased by methyl jasmonate (MeJA) treatment. These results suggest that CMe-DREB1 functions as a transcriptional activator of CMe-ACS2 in the presence of jasmonate.
|
